Plant‐type pentatricopeptide repeat proteins capable of C‐to‐U RNA editing perform faithfully when expressed in a new heterologous system, the yeast Saccharomyces cerevisiae. They were tested with constitutive and inducible expression and with a set of different solubility tags. PPR56, PPR65, and PPR78 from P.
Shyam Ramanathan +4 more
wiley +1 more source
Plasma virome of cattle from forest region revealed diverse small circular ssDNA viral genomes
Background Free-range cattle are common in the Northeast China area, which have close contact with farmers and may carry virus threatening to cattle and farmers.
Hao Wang +12 more
doaj +1 more source
Virus‐Induced Gene Silencing in Calendula officinalis (Pot Marigold)
ABSTRACT Virus‐induced gene silencing (VIGS) is a method that exploits plant antiviral defense mechanisms to downregulate endogenous genes. The technique is versatile, rapid, and widely used for functional genomics studies. Here, we report a method for VIGS in the medicinal plant, Calendula officinalis (pot marigold).
Daria Cuthbert +3 more
wiley +1 more source
Dynamics and Conformations of a Full-Length CRESS-DNA Replicase
Circular Rep-encoding single-stranded DNA (CRESS-DNA) viruses encode for a Replicase (Rep) that is essential for viral replication. Rep is a helicase with three domains: an endonuclease, an oligomeric, and an ATPase domain (ED, OD, and AD).
Reza Khayat, Elvira Tarasova
core +1 more source
Abstract Green tides caused by Ulva species have become one of the most serious marine ecological disasters, now impacting many coastal nations around the world. Although climatic and environmental drivers of these macroalgal blooms are well recognized, growing evidence identifies Ulva‐associated microbiota as potential pivotal regulators of bloom ...
Zhangyi Xia +6 more
wiley +1 more source
Influence of retinoblastoma-related gene silencing on the initiation of DNA replication by African cassava mosaic virus Rep in cells of mature leaves in Nicotiana benthamiana plants [PDF]
Background Geminiviruses mainly infect terminally differentiated tissues and cells in plants. They need to reprogramme host cellular machinery for DNA replication.
Yule Liu +14 more
core +1 more source
Single-Stranded DNA Viruses in Antarctic Cryoconite Holes
Antarctic cryoconite holes, or small melt-holes in the surfaces of glaciers, create habitable oases for isolated microbial communities with tightly linked microbial population structures. Viruses may influence the dynamics of polar microbial communities,
Pacifica Sommers +7 more
doaj +1 more source
Viral DNA genomes in sera of farrowing sows with or without stillbirths.
A study was conducted to investigate the serum virome of sows with and without stillbirths after farrowing. Sera from sows with at least one stillbirth or with normal litters were collected immediately after farrowing.
Caroline Tochetto +13 more
doaj +1 more source
A Democracia representativa: avanços e desafios - um estudo na comissão de políticas sociais do Conselho Regional de Serviço Social - 12 Região [PDF]
TCC (graduação) - Universidade Federal de Santa Catarina, Centro Sócio Econômico, Curso de Serviço SocialInvestiga os avanços e desafios da participação representativa junto a Comissão de Políticas Sociais do CRESS# 12." Região Realiza o debate sobre ...
Pereira, Maria Alice
core
Unveiling Crucivirus Diversity by Mining Metagenomic Data
The discovery of cruciviruses revealed the most explicit example of a common protein homologue between DNA and RNA viruses to date. Cruciviruses are a novel group of circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) viruses that encode capsid
Ignacio de la Higuera +26 more
doaj +1 more source

