Results 51 to 60 of about 389,860 (253)

CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation

, 2021
CRISPR-Cas pathways provide prokaryotes with acquired “immunity” against foreign genetic elements, including phages and plasmids. Although many of the proteins associated with CRISPR-Cas mechanisms are characterized, some requisite enzymes remain elusive.
Katerina Zabrady (5479505), Aidan Doherty (4461097), Matej Zabrady (5479511), Peter Kolesar (683818), Arthur Wing Hang Li (4466506)   +4 more
core  

Expanding the CRISPR toolbox for use within Bacillus subtilis [PDF]

, 2020
DNA editing is a vital tool in the development of biological systems for both research and commercial applications. Novel enabling tools accelerate strain engineering for the study of cellular mechanisms or production of small molecules and proteins ...
Price, Marcus Alexander
core   +1 more source

PreAcrs: a machine learning framework for identifying anti-CRISPR proteins [PDF]

, 2022
BACKGROUND: Anti-CRISPR proteins are potent modulators that inhibit the CRISPR-Cas immunity system and have huge potential in gene editing and gene therapy as a genome-editing tool. Extensive studies have shown that anti-CRISPR proteins are essential for
Wang, X, Li, Fuyi, Zhu, Lin, Zhu, L, Song, Jiangning, Zhu, L., Li, F., Song, J., Wang, X., Li, F, Wang, Xiaoyu, Song, J   +11 more
core   +1 more source

Proteasome inhibitor, ixazomib prevents topoisomerase‐I degradation and reverses irinotecan resistance in colorectal cancer

Molecular Oncology, EarlyView.
Ixazomib inhibits proteasome‐mediated degradation of topoisomerase I induced by irinotecan, thereby restoring drug sensitivity and promoting tumor cell death in colorectal cancer. Irinotecan, a topoisomerase I (topoI) inhibitor, is widely used for colorectal cancer, but resistance remains a major clinical challenge.
Yuho Ebata, Koji Ando, Hirofumi Hasuda, Koshi Mimori, Elizabeth C. Unan, Siddhartha Pulukuri, Aahana Tiku, Allison Berger, Eiji Oki, Ajit Bharti, Tomoharu Yoshizumi   +10 more
wiley   +1 more source

Prokaryotic homologs of Argonaute proteins are predicted to function as key components of a novel system of defense against mobile genetic elements

, 2009
Background In eukaryotes, RNA interference (RNAi) is a major mechanism of defense against viruses and transposable elements as well of regulating translation of endogenous mRNAs.
Makarova, K.S., Koonin Eugene V, Kira S Makarova, Makarova Kira S, Koonin, E.V., Oost, J., van der, John van der Oost, van der Oost, J., Yuri I Wolf, van der Oost John, Wolf, Y.I., Wolf Yuri I, Eugene V Koonin   +12 more
core   +1 more source

Detailed analysis of protein-DNA interactions driving type II-A CRISPR adaptation [PDF]

, 2020
CRISPR-Cas is an adaptive immune system that protects prokaryotes against foreign nucleic acids. Prokaryotes gain immunity by acquiring short pieces of the invading nucleic acid, termed prespacers, and inserting them into their CRISPR array using the ...
Van Orden, Mason
core  

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Highly Efficient CRISPR-Associated Protein 9 Ribonucleoprotein-Based Genome Editing in Euglena gracilis

STAR Protocols, 2020
Euglena gracilis, a unicellular phytoflagellate microalga, is a promising biomaterial for foods, feeds, and biofuels. However, targeted mutagenesis in this species has been a long-standing challenge. We recently developed a transgene-free, highly efficient, genome editing method for E. gracilis using CRISPR/Cas9 ribonucleoproteins (RNPs).
Toshihisa Nomura, Mizuki Yoshikawa, Kengo Suzuki, Keiichi Mochida   +3 more
openaire   +4 more sources

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

DECODING THE MOLECULAR WARFARE: ANTI-CRISPR PROTEINS AS GUARDIANS OF PRECISION IN CRISPR-CAS SYSTEMS [PDF]

The study explores the discovery, processes, evolution, and uses of anti-CRISPR (Acr) proteins, emphasizing the arms race between phage predation and bacterial defense mechanisms, particularly CRISPR-Cas systems.
Berryish Metha C., Dhanvarsha M.
core   +1 more source