Results 31 to 40 of about 389,860 (253)

Double-strand DNA end-binding and sliding of the toroidal CRISPR-associated protein Csn2 [PDF]

Nucleic Acids Research, 2013
The adaptive immunity of bacteria against foreign nucleic acids, mediated by CRISPR (clustered regularly interspaced short palindromic repeats), relies on the specific incorporation of short pieces of the invading foreign DNA into a special genomic locus, termed CRISPR array.
Arslan, Z., Wurm, R., Smits, S. H. J., Pul, U., Brener, O., Ellinger, P., Nagel-Steger, L., Oesterhelt, F., Schmitt, L., Willbold, D., Wagner, R., Gohlke, H.   +11 more
openaire   +3 more sources

Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia [PDF]

Nature Communications, 2019
AbstractThe delivery of biologic cargoes to airway epithelial cells is challenging due to the formidable barriers imposed by its specialized and differentiated cells. Among cargoes, recombinant proteins offer therapeutic promise but the lack of effective delivery methods limits their development.
Sateesh Krishnamurthy, Christine Wohlford-Lenane, Suhas Kandimalla, Gilles Sartre, David K. Meyerholz, Vanessa Théberge, Stéphanie Hallée, Anne-Marie Duperré, Thomas Del’Guidice, Jean-Pascal Lepetit-Stoffaes, Xavier Barbeau, David Guay, Paul B. McCray   +12 more
openaire   +3 more sources

Modified Cas (CRISPR-associated Protein) for Genome Editing and Beyond

Advanced Techniques in Biology & Medicine, 2015
As one of the most popular tools for genome editing, CRISPR/Cas9 system has been widely used in gene targeting, transcriptional regulation, epigenetic modification, even in gene therapy and drug delivery. Although CRISPR/Cas9 system provides a simple, specific and high efficient platform, it still has some limitations.
Tingfang Mei, Chu Jun Liu
openaire   +1 more source

Deactivated CRISPR Associated Protein 9 for Minor-Allele Enrichment in Cell-Free DNA [PDF]

Clinical Chemistry, 2018
Abstract BACKGROUND Cell-free DNA (cfDNA) diagnostics are emerging as a new paradigm of disease monitoring and therapy management. The clinical utility of these diagnostics is relatively limited by a low signal-to-noise ratio, such as with low allele frequency (AF) mutations in cancer. While enriching
Amin, Aalipour, Jonathan C, Dudley, Seung-Min, Park, Surya, Murty, Jacob J, Chabon, Evan A, Boyle, Maximilian, Diehn, Sanjiv S, Gambhir   +7 more
openaire   +2 more sources

Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity

, 2015
EC is supported by the Alexander von Humboldt Foundation, the German Federal Ministry for Education and Research, the Helmholtz Association, the Göran Gustafsson Foundation, the Swedish Research Council, the Kempe Foundation and Umeå University.
White, Malcolm F., Charpentier, Emmanuelle, White, Malcolm F, van der Oost, John, Richter, Hagen   +4 more
core   +1 more source

CRISPR-mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus

, 2014
European SulfoSYS-project [SysMo P–N-01-09-23] and grant [9P23000 and P25369] by the Austrian Research fund (to C.S.) and by grant [BB/K000314/1] from the Biotechnology and Biological Sciences Research Council (to M.F.W.). Funding for open access charge:
Manica, A., Zebec, Z., Schleper, C., Zhang, J., White, M.F.   +4 more
core   +1 more source

Nucleic acid binding surface and dimer interface revealed by CRISPR‐associated CasB protein structures [PDF]

FEBS Letters, 2012
TfuCasB1 and TfuCasB1 bind by molecular sieving (View Interaction: 1, 2)
Nam, Ki Hyun, Huang, Qingqiu, Ke, Ailong
openaire   +2 more sources

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Structure of the archaeal Cascade subunit Csa5 : Relating the small subunits of CRISPR effector complexes

, 2013
This work was funded by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC) (REF: BB/G011400/1) to M.F.W. and J.H.N. and a BBSRC-funded studentship to J.R.The Cascade complex for CRISPR-mediated antiviral immunity uses CRISPR ...
Naismith, Jim, White, Malcolm F, Anderson, Linzi, Liu, Huanting, Reeks, Judith Anne, Graham, Shirley   +5 more
core   +1 more source

Identification of proteins influencing CRISPR-associated transposases for enhanced genome editing

Science Advances
CRISPR-associated transposases (CASTs) hold tremendous potential for microbial genome editing because of their ability to integrate large DNA cargos in a programmable, site-specific manner. However, their widespread application has been hindered by poorly understood host factor requirements for transposition.
Leo C.T. Song, Amanda T.P. Alker, Agnès Oromí-Bosch, Sophia E. Swartz, Jonathan N.V. Martinson, Jigyasa Arora, Abby M. Wang, Rachel Rovinsky, Sara J. Smith, Emily C. Pierce, Adam M. Deutschbauer, Jennifer A. Doudna, Brady F. Cress, Benjamin E. Rubin   +13 more
openaire   +2 more sources