Results 21 to 30 of about 389,860 (253)

The CRISPR-associated Csx1 protein of Pyrococcus furiosus is an adenosine-specific endoribonuclease [PDF]

RNA, 2015
Prokaryotes are frequently exposed to potentially harmful invasive nucleic acids from phages, plasmids, and transposons. One method of defense is the CRISPR-Cas adaptive immune system. Diverse CRISPR-Cas systems form distinct ribonucleoprotein effector complexes that target and cleave invasive nucleic acids to provide immunity.
Nolan F. Sheppard, Claiborne V.C. Glover, Rebecca M. Terns, Michael P. Terns   +3 more
openaire   +2 more sources

CRISPR interference : a structural perspective [PDF]

, 2013
This article was made open access through BIS OA funding. The laboratory is funded by grants from the Biotechnology and Biological Sciences Research Council (BBSRC).CRISPR (cluster of regularly interspaced palindromic repeats) is a prokaryotic adaptive ...
Naismith, Jim, White, Malcolm F, Reeks, Judith Anne   +2 more
core   +1 more source

Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6 [PDF]

RNA, 2016
Prokaryotic CRISPR–Cas systems provide an RNA-guided mechanism for genome defense against mobile genetic elements such as viruses and plasmids. In type III-A CRISPR–Cas systems, the RNA-guided multisubunit Csm effector complex targets both single-stranded RNAs and double-stranded DNAs.
Niewoehner, Ole, Jinek, Martin
openaire   +4 more sources

The CRISPR-associated Cas4 protein from Leptospira interrogans demonstrate versatile nuclease activity

Current Research in Microbial Sciences, 2021
The Cas4 protein is one of the core CRISPR-associated (Cas) proteins implicated in the adaptation module in many variants of the CRISPR-Cas system in prokaryotes against the invading genetic elements. Cas4 is recognized as a DNA exonuclease that contains a RecB nuclease domain and a Fe-S cluster-binding module.
Bhuvan Dixit, Vineet Anand, Md. Saddam Hussain, Manish Kumar   +3 more
openaire   +3 more sources

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition [PDF]

, 2015
This work was supported by a grant from the Biotechnology and Biological Sciences Research Council (REF: BB/M000400/1 to MFW).The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements.
Bolt, Edward, White, Malcolm F., Rollie, C, White, Malcolm F, Anna Sophie Brinkmann, Malcolm F White, Clare Rollie, Edward L Bolt, Brinkmann, Anna Sophie, Rollie, Clare, Bolt, EL, Rollie, Clare Jane Catherine, Schneider, Stefanie, White, MF, Stefanie Schneider, Bolt, Edward L., Schneider, S, Brinkmann, AS   +17 more
core   +1 more source

Structure and RNA-binding properties of the Type III-A CRISPR-associated protein Csm3 [PDF]

RNA Biology, 2013
The prokaryotic adaptive immune system is based on the incorporation of genome fragments of invading viral genetic elements into clusters of regulatory interspaced short palindromic repeats (CRISPRs). The CRISPR loci are transcribed and processed into crRNAs, which are then used to target the invading nucleic acid for degradation.
Hrle, A., Su, A., Ebert, J., Benda, C., Randau, L., Conti, E.   +5 more
openaire   +4 more sources

Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease [PDF]

Biology Direct, 2015
Many proteins of viruses infecting hyperthermophilic Crenarchaeota have no detectable homologs in current databases, hampering our understanding of viral evolution. We used sensitive database search methods and structural modeling to show that a nucleocapsid protein (TP1) of Thermoproteus tenax virus 1 (TTV1) is a derivative of the Cas4 nuclease, a ...
Krupovic, Mart, Cvirkaite-Krupovic, Virginija, Prangishvili, David, Koonin, Eugene V.   +3 more
openaire   +3 more sources

Structural basis for CRISPR RNA-guided DNA recognition by Cascade [PDF]

, 2011
The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids.
Beijer, Marieke R., Mark J Dickman, Dickman, Mark J.,, Snijders, AP, Matthijs M Jore, Rolf Wagner, Sakharam P Waghmare, Beijer, Marieke, Wagner, Rolf, Brouns, Stan J. J., Barendregt, Arjan,, Snijders, Ambrosius, Dickman, Mark, Oost, J., van der, Arjan Barendregt, Boekema, Egbert, Dickman, Mark J., Bultema, J.B., Boekema, EJ, Waghmare, Sakharam P.,, Brouns, S.J.J., Wurm, Reinhild,, Westra, ER, Pul, U, Boekema, Egbert J., Doudna, Jennifer, Pul, Ümit,, Beijer, Marieke R.,, Egbert J Boekema, Jelle B Bultema, Westra, Edze, Snijders, Ambrosius P.L.,, Lundgren, Magnus, Jennifer A Doudna, Bultema, Jelle B.,, Wagner, R, John van der Oost, Westra, Edze R.,, van der Oost, J, Beijer, M.R., Wurm, R, Beijer, MR, Edze R Westra, Doudna, Jennifer A.,, Kaihong Zhou, Duijn, E., van, Jore, M.M., Heck, AJ, van der Oost, J., Waghmare, Sakharam, Jore, MM, Westra, E.R., Brouns, SJ, Doudna, Jennifer A., Wurm, Reinhild, Wagner, Rolf,, Ambrosius P L Snijders, Zhou, K, Barendregt, Arjan, Albert J R Heck, Brouns, Stan J.J.,, Magnus Lundgren, Pul, Uemit, Boekema, Egbert J.,, van Duijn, Esther, Snijders, Ambrosius P. L., Heck, Albert J. R., Lundgren, M, Pul, Ümit, Zhou, Kaihong,, Oost, John van der,, Blake Wiedenheft, Wiedenheft, Blake,, Wiedenheft, Blake, Waghmare, SP, van der Oost, John, Reinhild Wurm, Stan J J Brouns, Brouns, Stan, Jore, Matthijs M.,, Waghmare, Sakharam P., Bultema, Jelle, Zhou, Kaihong, Dickman, MJ, Lundgren, Magnus,, Barendregt, A, Bultema, JB, Jore, Matthijs, Duijn, Esther van,, Jore, Matthijs M., Lundgren, N.M.J., Ümit Pul, Marieke R Beijer, Wiedenheft, B, van Duijn, E., Heck, Albert J.R.,, Heck, Albert, Bultema, Jelle B., van Duijn, E, Westra, Edze R., Esther van Duijn, Doudna, JA   +101 more
core   +1 more source

Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9 [PDF]

, 2015
CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high
Rojas-Fernandez, Alejandro, Hay, Ronald T., Macartney, Thomas, Herhaus, Lina, Sapkota, Gopal P., Lachaud, Christophe   +5 more
core   +1 more source

The Structure of the CRISPR-Associated Protein Csa3 Provides Insight into the Regulation of the CRISPR/Cas System [PDF]

Journal of Molecular Biology, 2011
Adaptive immune systems have recently been recognized in prokaryotic organisms where, in response to viral infection, they incorporate short fragments of invader-derived DNA into loci called clustered regularly interspaced short palindromic repeats (CRISPRs).
Nathanael G, Lintner, Kenneth A, Frankel, Susan E, Tsutakawa, Donald L, Alsbury, Valérie, Copié, Mark J, Young, John A, Tainer, C Martin, Lawrence   +7 more
openaire   +2 more sources