Results 11 to 20 of about 389,860 (253)

Phage-induced expression of CRISPR-associated proteins is revealed by shotgun proteomics in streptococcus thermophilus [PDF]

PLoS ONE, 2012
The CRISPR/Cas system, comprised of clustered regularly interspaced short palindromic repeats along with their associated (Cas) proteins, protects bacteria and archaea from viral predation and invading nucleic acids.
Banfield, JF, Horvath, P, VerBerkmoes, NC, Fremaux, C, Shah, M, Dill, BD, Barrangou, R, Hettich, RL, Young, JC, Pan, C   +9 more
core   +8 more sources

Taxonomic Distribution, Phylogenetic Relationship, and Domain Conservation of CRISPR-Associated Cas Proteins

Bioinformatics and Biology Insights
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a naturally occurring genetic defense system in bacteria and archaea. It is comprised of a series of DNA sequence repeats with spacers derived from previous exposures to plasmid or ...
Hyuk Cho, Dorcie Gillette, Alexis Ho, Weerakkody Ranasinghe, Madhusudan Choudhary   +4 more
core   +4 more sources

Functional roles of intrinsic disorder in CRISPR-associated protein Cas9

Molecular BioSystems, 2017
Protein intrinsic disorder is an important characteristic commonly detected in multifunctional or RNA- and DNA-binding proteins. We show here that the CRISPR-associated Cas9 proteins of different origin contain functionally important intrinsically ...
Uversky, Vladimir N., Zhihua Du, Vladimir N. Uversky, Du, Zhihua   +3 more
core   +4 more sources

Engineering plants using diverse CRISPR-associated proteins and deregulation of genome-edited crops

Trends in Biotechnology
The CRISPR/Cas system comprises RNA-guided nucleases, the target specificity of which is directed by Watson–Crick base pairing of target loci with single guide (sg)RNA to induce the desired edits. CRISPR-associated proteins and other engineered nucleases
Jones, M.G.K, Lozano-Juste, Jorge, Chao, L, Varshney, R.K, Wang, H.F, Zaman, Q.U, Raza, A   +6 more
core   +7 more sources

Part A: Application of fluorescent markers onto CRISPR-associated proteins/ Part B: Substitution of cysteine residues in CRISPR associated Protein Cas1 [PDF]

Part A: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems mediate prokaryotic adaptive immunity against mobile genetic elements (MGEs), where MGE sequences are captured and stored as spacers in CRISPR loci.
Parr, Lochlan
core   +3 more sources

RNA-activated protein cleavage with a CRISPR-associated endopeptidase

Science, 2022
In prokaryotes, CRISPR-Cas systems provide adaptive immune responses against foreign genetic elements through RNA-guided nuclease activity. Recently, additional genes with non-nuclease functions have been found in genetic association with CRISPR systems, suggesting that there may be other RNA-guided non-nucleolytic enzymes.
Jonathan Strecker, F. Esra Demircioglu, David Li, Guilhem Faure, Max E. Wilkinson, Jonathan S. Gootenberg, Omar O. Abudayyeh, Hiroshi Nishimasu, Rhiannon K. Macrae, Feng Zhang   +9 more
openaire   +2 more sources

Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins

Nature Microbiology, 2023
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9 is an effector protein that targets invading DNA and plays a major role in the prokaryotic adaptive immune system. Although Streptococcus pyogenes CRISPR-Cas9 has been widely studied and repurposed for applications including genome editing, its origin and evolution are ...
Borja Alonso-Lerma, Ylenia Jabalera, Sara Samperio, Matias Morin, Almudena Fernandez, Logan T. Hille, Rachel A. Silverstein, Ane Quesada-Ganuza, Antonio Reifs, Sergio Fernández-Peñalver, Yolanda Benitez, Lucia Soletto, Jose A. Gavira, Adrian Diaz, Wim Vranken, Avencia Sanchez-Mejias, Marc Güell, Francisco J. M. Mojica, Benjamin P. Kleinstiver, Miguel A. Moreno-Pelayo, Lluis Montoliu, Raul Perez-Jimenez   +21 more
openaire   +6 more sources

Programmable mammalian translational modulators by CRISPR-associated proteins [PDF]

, 2021
Abstract The complexity of synthetic genetic circuits relies on repertories of biological circuitry with high orthogonality. Although post-transcriptional circuitry relying on RNA-binding proteins (RBPs) qualifies as a repertory, the limited pool of regulatory devices hinders network modularity and scalability. Here we propose CaRTRIDGE
Shunsuke Kawasaki, Hiroki Ono, Moe Hirosawa, Takeru Kuwabara, Hirohide Saito   +4 more
openaire   +1 more source

Functionalizing Cell-Free Systems with CRISPR-Associated Proteins: Application to RNA-Based Circuit Engineering [PDF]

ACS Synthetic Biology, 2021
Abstract Cell-free systems have become a compelling choice for the prototyping of synthetic circuits. Many robust protocols for preparing cell-free systems are now available along with toolboxes designed for a variety of applications.
Lehr, François-Xavier, Kuzembayeva, Alina, Bailey, Megan E., Kleindienst, Werner, Kabisch, Johannes, Koeppl, Heinz   +5 more
openaire   +2 more sources

Structure of a CRISPR-associated protein Cas2 fromDesulfovibrio vulgaris [PDF]

Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2010
CRISPRs (clustered regularly interspaced short palindromic repeats) provide bacteria and archaea with RNA-guided acquired immunity to invasive DNAs. CRISPR-associated (Cas) proteins carry out the immune effector functions. Cas2 is a universal component of the CRISPR system.
Poulami, Samai, Paul, Smith, Stewart, Shuman   +2 more
openaire   +2 more sources