Results 201 to 210 of about 50,942 (257)

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Erratum: Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing [Corrigendum]. [PDF]

Biologics
europepmc   +1 more source

Illuminating the genome: emerging approaches in CRISPR-Cas live-cell imaging. [PDF]

Nucleic Acids Res
Xiao Z, Sun Y.
europepmc   +1 more source

Engineering delivery platforms for CRISPR-Cas and their applications in healthcare, agriculture and beyond. [PDF]

Nanoscale Adv
Bharti N, Modi U, Bhatia D, Solanki R.
europepmc   +1 more source

HapAsmbl: A reference‐aided pipeline for assembling haplotypes in Nanopore amplicon sequence data of polymorphic populations

Applications in Plant Sciences, EarlyView.
Abstract Premise Advances in long‐read sequencing offer new possibilities to investigate haplotype diversity across multiple genes in plants and other taxa through multi‐locus, long‐read amplicon sequencing (multi‐locus LRAS). Despite this progress, there is a notable absence of dedicated bioinformatics pipelines for assembling diploid haplotypes of ...
Ayodele Oluwaseyi Fakoya, Augustine Chen, Rowan Paul Herridge, Richard Colin Macknight, Lynette Ruth Brownfield   +4 more
wiley   +1 more source

The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding

Animal Research and One Health, EarlyView.
A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang, Weiran Wu, Qingqing Cai, Jiaxing Wang, Qi Gao, Muhammad Iftikhar, Yulin Bi, Hao Bai, Lihong Gu, Guobin Chang, Shihao Chen   +10 more
wiley   +1 more source

CRISPR-Cas systems against carbapenem resistance: from proof-of-concept to clinical translation. [PDF]

Front Microbiol
Tsolakidou PJ.
europepmc   +1 more source

Recent Advances in Virus–Host Interactions, Antiviral Bioactive Compounds, and Breeding for Disease Resistance of Porcine Epidemic Diarrhea Virus

Animal Research and One Health, EarlyView.
Basic research on the PEDV infection cycle and virus–host interactions advances the development of anti‐PEDV drugs and disease‐resistant breeding and helps strengthen disease prevention and control while reducing economic losses in the swine industry.
Heyong Wu, Aosi Qi, Shuyu Zhong, Haozhen Du, Shimeng Guo, Hongsheng Ouyang, Hongming Yuan, Daxin Pang, Zicong Xie   +8 more
wiley   +1 more source

CRISPR-Cas-associated SCCmec Variants in Methicillin-resistant Staphylococcus aureus Evade Rapid Diagnostic Detection. [PDF]

J Infect Dis
Podkowik M, Tillman A, Takats C, Carion H, Putzel G, McWilliams J, See B, Wang G, Munoz-Gomez S, Otto C, Drlica K, Marraffini L, Pironti A, Hochman S, Kerantzas C, Shopsin B.   +15 more
europepmc   +1 more source

Advances and challenges of CRISPR/Cas gene editing for corneal diseases. [PDF]

Adv Ophthalmol Pract Res
Han Y, Chen R, Shentu X.
europepmc   +1 more source