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Methods, 2020
Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins (Cas) are essential genetic elements in many archaeal and bacterial genomes, playing a key role in a prokaryote adaptive immune system against invasive foreign elements.
Alkhnbashi, Omer S. +4 more
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Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins (Cas) are essential genetic elements in many archaeal and bacterial genomes, playing a key role in a prokaryote adaptive immune system against invasive foreign elements.
Alkhnbashi, Omer S. +4 more
openaire +3 more sources
CRISPR-Cas-mediated diagnostics
Trends in Biotechnology, 2022An ideal molecular diagnostic method should be sensitive, specific, low cost, rapid, portable, and easy to operate. Traditional nucleic acid detection methods based mainly on PCR technology have not only high sensitivity and specificity, but also some limitations, such as the need for expensive equipment and skilled technicians, being both time and ...
Luoxi, Li +5 more
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Chemie in unserer Zeit, 2017
ZusammenfassungCRISPR‐Cas ist ein adaptives und vererbbares Immunsystem, mit dem sich Prokaryoten gegen invasive genetische Elemente wie Viren wehren. Es eignet sich aber auch hervorragend zur Manipulation von Genomen. Die Nuklease Cas9 wird durch eine Leit‐RNA zu einem bestimmten DNA‐Abschnitt geführt und fügt dort einen Doppelstrangbruch ein.
Larissa Tetsch +2 more
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ZusammenfassungCRISPR‐Cas ist ein adaptives und vererbbares Immunsystem, mit dem sich Prokaryoten gegen invasive genetische Elemente wie Viren wehren. Es eignet sich aber auch hervorragend zur Manipulation von Genomen. Die Nuklease Cas9 wird durch eine Leit‐RNA zu einem bestimmten DNA‐Abschnitt geführt und fügt dort einen Doppelstrangbruch ein.
Larissa Tetsch +2 more
openaire +2 more sources
Biologie in unserer Zeit, 2013
AbstractEiner der ältesten Konflikte auf Erden ist vermutlich der Existenzkampf zwischen Prokaryoten und Viren. Prokaryoten haben verschiedene Abwehrstrategien entwickelt, um sich gegen die allgegenwärtigen und zahlenmäßig überlegenen Viren zu schützen. Letztere besitzen wiederum Gegenmechanismen, mit denen sie den prokaryotischen Schutz umgehen.
Anita Marchfelder +3 more
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AbstractEiner der ältesten Konflikte auf Erden ist vermutlich der Existenzkampf zwischen Prokaryoten und Viren. Prokaryoten haben verschiedene Abwehrstrategien entwickelt, um sich gegen die allgegenwärtigen und zahlenmäßig überlegenen Viren zu schützen. Letztere besitzen wiederum Gegenmechanismen, mit denen sie den prokaryotischen Schutz umgehen.
Anita Marchfelder +3 more
openaire +1 more source
MicroRNA Detection with CRISPR/Cas
2023Low-cost detection of miRNAs has caught broad attention in recent years due to the potential application of these small noncoding RNAs for diagnostics and therapeutic purposes. Their small size and low abundance, however, derive challenges in engineering robust detection tools.
Xinyuan, Qiu +3 more
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CRISPR–Cas in the laboratory classroom
Nature Microbiology, 2017CRISPR–Cas is an adaptive immune system found in Bacteria and Archaea that confers sequence-specific protection against invasion by foreign nucleic acids. CRISPR–Cas is also a powerful tool for microbiology education and has been an inspiring model for our students for the past three years.
Luc, Trudel +2 more
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CRISPR-Cas immunity in prokaryotes
Nature, 2015Prokaryotic organisms are threatened by a large array of viruses and have developed numerous defence strategies. Among these, only clustered, regularly interspaced short palindromic repeat (CRISPR)-Cas systems provide adaptive immunity against foreign elements. Upon viral injection, a small sequence of the viral genome, known as a spacer, is integrated
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Journal of Cellular Physiology, 2020
AbstractClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated enzyme (Cas) is a naturally occurring genome editing tool adopted from the prokaryotic adaptive immune defense system. Currently, CRISPR/Cas9‐based genome editing has been becoming one of the most promising tools for treating human genetic diseases, including ...
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AbstractClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated enzyme (Cas) is a naturally occurring genome editing tool adopted from the prokaryotic adaptive immune defense system. Currently, CRISPR/Cas9‐based genome editing has been becoming one of the most promising tools for treating human genetic diseases, including ...
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Staying on target with CRISPR-Cas
Nature Biotechnology, 2013Four independent studies shed light on the specificity of RNA-guided genome editing tools based on the Streptococcus pyogenes Cas9 protein.
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