Results 151 to 160 of about 7,068 (190)

Nuc domain electrostatics drive the trans cleavage activity of CRISPR-Cas12a. [PDF]

Nucleic Acids Res
Newman A, Starrs L, Burgio G.
europepmc   +1 more source

A CRISPR-Cas12a amplified RNase activity sensor powered by gold nanoparticle-barcode DNA multipliers.

Mater Adv
Munusamy S, Jahani R, Chen J, Zhou S, Kong J, Zheng H, Guan X.   +6 more
europepmc   +1 more source

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Mini crRNA-mediated CRISPR/Cas12a system (MCM-CRISPR/Cas12a) and its application in RNA detection

Talanta, 2023
Some non-coding RNAs are abnormally expressed during the occurrence and development of diseases, so it is necessary to develop analytical methods that can specifically and sensitively detect them. In typical CRISPR/Cas12a system, a complete crRNA that can recognize single-stranded or double-stranded DNA is necessary to activate its trans-cleavage ...
Xiaolong, Chen, Chaowang, Huang, Jing, Zhang, Qiao, Hu, Dan, Wang, Qianyi, You, Yawen, Guo, Huaping, Chen, Jing, Xu, Mingdong, Hu   +9 more
openaire   +2 more sources

CRISPR‐Cas12a System for Biosensing and Gene Regulation

Chemistry – An Asian Journal, 2021
AbstractClustered regularly interspaced short palindromic repeats (CRISPR) is a promising technology in the biological world. As one of the CRISPR‐associated (Cas) proteins, Cas12a is an RNA‐guided nuclease in the type V CRISPR‐Cas system, which has been a robust tool for gene editing.
Yuyan Shi, Xiaoyi Fu, Yao Yin, Fangqi Peng, Xia Yin, Guoliang Ke, Xiaobing Zhang   +6 more
openaire   +2 more sources

Application of CRISPR/Cas12a in the rapid detection of pathogens

Clinica Chimica Acta, 2023
The combination of clustered regularly interspaced short palindromic repeats (CRISPR) and its associated Cas protein is an effective gene-editing instrument. Among them, the CRISPR-Cas12a system forms a DNA-cleavage-capable complex with crRNA and exerts its trans-cleavage activity by recognising the PAM site on the target pathogen's gene.
Yiheng Wang, Tianmu Yang, Guifang Liu, Longfei Xie, Jianying Guo, Wenguang Xiong   +5 more
openaire   +2 more sources

Programmable Aptasensor for Regulating CRISPR/Cas12a Activity

ACS Sensors, 2023
CRISPR-mediated aptasensors have gained prevalence for detecting non-nucleic acid targets. However, there is an urgent need to develop an easily customizable design to improve the signal-to-noise ratio, enhance universality, and expand the detection range. In this article, we report a CRISPR-mediated programmable aptasensor (CPAS) platform.
Yao Yu, Qiaoyu Li, Wen Shi, Yuxin Yang, Hongpeng He, Junbiao Dai, Guobin Mao, Yingxin Ma   +7 more
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CRISPR-Cas12a: Functional overview and applications

Biomedical Journal, 2020
Prokaryotes have developed an adaptive immune system called Clustered regularly interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic elements (MGEs) such as plasmids and phages. In the past decade, the widely characterized CRISPR-Cas9 enzyme has been redesigned to trigger a genome editing revolution.
Guillermo Montoya, Bijoya Paul
exaly   +5 more sources

Rapid and sensitive exosome detection with CRISPR/Cas12a

Analytical and Bioanalytical Chemistry, 2020
Numerous studies have shown that exosomes are closely related to the pathogenesis of various diseases, especially cancers. Therefore, a rapid and sensitive method for exosome detection will be of great importance for the diagnosis and prognosis of diseases.
Xianxian, Zhao, Wenqing, Zhang, Xiaopei, Qiu, Qiang, Mei, Yang, Luo, Weiling, Fu   +5 more
openaire   +2 more sources