Results 161 to 170 of about 7,068 (190)

การพัฒนาเทคนิคการตรวจหาเชื้อไวรัสไข้หวัดใหญ่และเชื้อไวรัสโคโรนาสายพันธุ์ใหม่โดยใช้เทคโนโลยี CRISPR-Cas12a

2023
ในงานวิจัยนี้ ได้ประยุกต์ใช้เทคนิค CRISPR-Cas12a ซึ่งเป็นเทคนิคที่ง่าย รวดเร็ว และประหยัดค่าใช้จ่าย ในการตรวจหาเชื้อไวรัสไข้หวัดใหญ่ และเชื้อไวรัสโคโรนาสายพันธุ์ใหม่ จึงเหมาะสำหรับการตรวจคัดกรองผู้ป่วยโรคไข้หวัดใหญ่ และ โควิด-19 ในบริเวณที่มีอุปกรณ์ทางการแพทย์ที่จำกัด ผลการทดสอบพบว่า การใช้เทคนิค RT-RPA ร่วมกับ CRISPR-Cas12a ตรวจหาเชื้อไวรัสโคโรนาสายพ ...
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MXene Coupled with CRISPR-Cas12a for Analysis of Endotoxin and Bacteria

Analytical Chemistry, 2021
With hydrophilic surface and high density of functional groups, MXene can efficiently adsorb single-stranded DNA to enhance target-induced strand release and quench the fluorescence. Herein, MXene is coupled with CRISPR-Cas12a to sensitively detect LPS and bacteria.
Anzhi Sheng, Pei Wang, Jingyi Yang, Longfei Tang, Feng Chen, Juan Zhang   +5 more
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An ultrasensitive homogeneous electrochemical biosensor based on CRISPR-Cas12a

Analytical Methods, 2021
A simple and immobilization-free homogeneous electrochemical biosensor for the detection of human papillomavirus (HPV-16) has been fabricated by combining with methylene blue (MB)-labeled probes toward the negatively-charged indium tin oxide (ITO) electrode.
Jie Liu, Qing Wan, Ruijin Zeng, Dianping Tang   +3 more
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CRISPR‐Cas12a assisted specific detection of mpox virus

Journal of Medical Virology, 2023
AbstractMpox virus, a member of genus Orthopoxvirus, causes rash and flu‐like symptoms in humans. In the recent global outbreak, it was reported from several geographical areas that have not historically reported mpox. Point of care, sensitive and specific mpox diagnostic assays are critical in checking the spread of the disease.
Mandeep Singh, Chitra Seetharam Misra, Gargi Bindal, Shyam Sunder Rangu, Devashish Rath   +4 more
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Split technology in sensors based on CRISPR/Cas12a system

Biotechnology Advances
CRISPR/Cas12a system has become a popular tool for nucleic acid analysis in recent years due to its high specificity, sensitivity and programmability. Recently, split technology has been applied to the CRISPR/Cas12a system for activators, crRNA, reporter and Cas12a.
Songcheng Yu, Xueying Lei, Xianmin Ding
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Metagenome-Derived CRISPR-Cas12a Mining and Characterization

The CRISPR Journal
The advent of clustered regularly interspaced short palindromic repeats (CRISPR)-based technologies has revolutionized genome editing, with continued interest in expanding the CRISPR-associated proteins (Cas) toolbox with diverse, efficient, and specific effectors.
Kalani Gast, Sydney Baker, Adair L. Borges, Stephanie Ward, Jillian F. Banfield, Rodolphe Barrangou   +5 more
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CRISPR-Cas12a-Based Diagnostics of Wheat Fungal Diseases

Journal of Agricultural and Food Chemistry, 2022
Fusarium head blight (FHB) of wheat, mainly caused by Fusarium graminearum (F. graminearum) infection, reduces crop yield and contaminates grain with mycotoxins. We report a clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a-based nucleic acid assay for an early and rapid diagnosis of wheat FHB.
Keqing Mu, Xiaojun Ren, Hao Yang, Ting Zhang, Weiyi Yan, Fengping Yuan, Jianhui Wu, Zhensheng Kang, Dejun Han, Ruijie Deng, Qingdong Zeng   +10 more
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CRISPR-Cas12a/Cas13a in cancer molecular diagnosis

Clinica Chimica Acta
Cancer remains a leading cause of global mortality, with early diagnosis being pivotal for improving treatment outcomes. Traditional tissue biopsy is limited by its invasiveness, inability to capture tumor heterogeneity, and failure to support dynamic monitoring.
Juncheng, Lin, Yuyuan, Wang, Biyun, Zeng, Zhibing, Chen, Xiaocong, Lin, Tao, Zeng   +5 more
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An EXPAR-CRISPR/Cas12a Assay for Rapid Detection of Salmonella

Current Microbiology
Salmonella is considered as one of the primary pathogens associated with foodborne diseases globally. The effective treatment of these illnesses depends on the rapid and accurate identification of this organism. Traditional culture methods, however, necessitate extended testing periods, while many alternative techniques often lack precision.
Wensen Lin, Mintao Huang, Hongjian Fu, Luxin Yu, Ying Chen, Lingwei Chen, Yanzhen Lin, Ting Wen, Xiaomin Luo, Yanguang Cong   +9 more
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A CRISPR/Cas12a-based competitive aptasensor for ochratoxin A detection

Analytical Methods
A competitive aptasensor for ochratoxin A (OTA) using CRISPR/Cas12a as a signal amplifier with a streptavidin bridging aptamer and a DNA activator.
Fengxi Zhu, Qiang Zhao
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