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One-Pot Assay Based on CRISPR/Cas13a Technology for HEV RNA Point-of-Care Testing. [PDF]
J Med VirolFan Z +13 more
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A New Tool for CRISPR-Cas13a-Based Cancer Gene Therapy [PDF]
Molecular Therapy - Oncolytics, 2020 Cas13a has already been successfully applied to virus detection. However, as a new gene interference tool, its potential in cancer treatment was not fully explored until now. This study constructed a new Cas13a expression vector, decoy minimal promoter-Cas13a-U6-guide RNA (DMP-Cas13a-U6-gRNA [DCUg]), by controlling the Cas13a and gRNA expression with a
Tao Luo, Jinke Wang, Jinliang Gao
exaly +5 more sources
The CRISPR-Cas13a system interferes with Purkinje cell dendritic development
Biochimica Et Biophysica Acta - Molecular Cell Research, 2020 Josef P Kapfhammer, Qin-Wei Wu
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Structure of AcrVIA2 and its binding mechanism to CRISPR-Cas13a
Biochemical and Biophysical Research Communications, 2022Phages and non-phage derived bacteria have evolved many anti-CRISPR proteins (Acrs) to escape the adaptive immune system of prokaryotes. Thus Acrs can be applied as a regulatory tool for gene edition by CRISPR system. Recently, a non-phage derived AcrVIA2 has been identified as an inhibitor that blocks the editing activity of Cas13a in vitro by binding
Guangyong Song +5 more
openaire +2 more sources
RNA-mediated Allosteric Activation in CRISPR-Cas13a
2023ABSTRACT Cas13a is a recent addition to the CRISPR-Cas toolkit that exclusively targets RNA, which makes it a promising tool for RNA detection. The protein uses a CRISPR RNA (crRNA) to target RNA sequences, which are cleaved by a composite active site formed by two ‘Higher Eukaryotes and Prokaryotes Nucleotide’ (HEPN) catalytic domains.
Souvik Sinha +5 more
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Sensitive detection of foodborne pathogens based on CRISPR‐Cas13a
Journal of Food Science, 2021Abstract Salmonella , being one of the most widespread foodborne pathogens, is a compulsory test item required by national food safety standard of China and many other countries.
Song Gao +4 more
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Rapid detection of Nipah virus using the one-pot RPA-CRISPR/Cas13a assay
Virus Research, 2023 International audienceNipah virus (NiV) is a zoonotic pathogen with airborne transmission and high case fatality rates in humans. There is currently no treatment or vaccine against NiV infection approved for humans or animals, therefore early diagnosis ...
Gary Wong, Dongmei He
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Rapid and facile detection of HBV with CRISPR/Cas13a
New Journal of Chemistry, 2022A rapid point-of-care assay based on CRISPR-Cas13a for HBV was built with a LOD of 1 copy per μL was achieved in 50 min. The lateral flow test strip method can achieve naked-eye results, and the fluorescence readout can achieve real-time detection.
Ronghua Ding +5 more
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CRISPR–Cas13a: Prospects for Plant Virus Resistance
Trends in Biotechnology, 2018CRISPR-Cas13a is an efficient RNA targeting and editing tool characterized recently in prokaryotes. This system can be recruited to engineer resistance against plant RNA viruses and regulate gene expression. We discuss some important achievements in using the CRISPR-Cas13a system to confer resistance against plant RNA viruses.
Muhammad Zuhaib, Khan +3 more
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CRISPR/Cas13a-Based MicroRNA Detection in Tumor-Derived Extracellular Vesicles
Advanced Science, 2023 AbstractMicroRNAs (miRNAs) in extracellular vesicles (EVs) play essential roles in cancer initiation and progression. Quantitative measurements of EV miRNAs are critical for cancer diagnosis and longitudinal monitoring. Traditional PCR‐based methods, however, require multi‐step procedures and remain as bulk analysis.
Hyungsoon Im +2 more
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