Results 161 to 170 of about 83,279 (184)
Some of the next articles are maybe not open access.
Electrochemical biosensor for CRISPR/Cas13a powered miRNA diagnostics
2019 IEEE SENSORS, 2019Non-coding small RNAs, such as microRNAs, are becoming the biomarkers of choice for multiple diseases in clinical diagnostics. A dysregulation of these microRNAs can be associated to many different diseases, such as cancer, dementia or cardiovascular conditions. The key issue for an effective treatment is an accurate initial diagnosis at an early stage,
Richard Bruch +5 more
openaire +2 more sources
CRISPR/Cas13a targeting of RNA virus in plants
Plant Cell Reports, 2018This approach is quite promising to control plant viral diseases and create synthetic networks to better understand the structure/function relationship in RNA and proteins. Plant viruses are obligate intracellular parasites which causes enormous losses in crop yield worldwide.
openaire +2 more sources
Locus-specific detection of pseudouridine with CRISPR-Cas13a
Chemical CommunicationsA streamlined and efficient method enables precise detection of pseudouridine (Ψ) at specific loci, by leveraging CRISPR-Cas13a and CMC chemical labeling for immediate fluorescence analysis.
Mei Sun +6 more
openaire +2 more sources
CRISPR/Cas13a Analysis Based on NASBA Amplification for Norovirus Detection
TalantaHuman norovirus (HuNoV) is a leading cause of foodborne diseases worldwide, making rapid and accurate detection crucial for prevention and control. In recent years, the CRISPR/Cas13a system, known for its single-base resolution in RNA recognition and unique collateral cleavage activity, is particularly suitable for sensitive and rapid RNA detection ...
Zefeng, Mao +5 more
openaire +2 more sources
Application of CRISPR/Cas13a-based biosensors in serum marker detection
Analytical MethodsThis paper focuses on the research progress of CRISPR/Cas13a-based biosensors in serum marker detection, their principles and applications, the differences between the different types of these biosensors, and their future development direction.
Wei He +5 more
openaire +2 more sources
Rapid and facile detection of largemouth bass ranavirus with CRISPR/Cas13a
Fish & Shellfish ImmunologyLargemouth bass ranavirus (LMBV) is an epidemic disease that seriously jeopardizes the culture of largemouth bass（Micropterus salmoides）, and it has a very high incidence in largemouth bass. Once an outbreak occurs, it may directly lead to the failure of the culture, resulting in substantial economic losses, but there is no effective vaccine or special
Min Guang +10 more
openaire +2 more sources
A review on the mechanism and potential diagnostic application of CRISPR/Cas13a system
Mammalian GenomeClustered regularly Interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins form a natural immune defense system in prokaryotic species, with approximately 90% of archaea and 40% of bacteria possessing these systems, highlighting their widespread role in microbial immunity. Among these, the CRISPR/Cas13a system, guided by a
Abdul, Basit +3 more
openaire +2 more sources
Detecting CYP2C19 genes through an integrated CRISPR/Cas13a-assisted system
Analytical MethodsA platform integrating PCR reaction and CRISPR/Cas13a detection of three CYP2C19 genotypes on a device based on magnetic beads of three different sizes, achieving rapid, sensitive, and specific detection.
Zhaokang Mai, Tao Zhou, Zhun Lin
openaire +2 more sources
Rapid on-site detection of crop RNA viruses using CRISPR/Cas13a
Journal of Experimental BotanyAbstract Plant viruses are destructive pathogens of various crop species. Rapid, sensitive, and specific detection is crucial for the effective containment of emerging and resistance-breaking viruses. CRISPR/Cas has been established as a new tool for plant virus identification.
Hagit Hak +6 more
openaire +2 more sources