Results 191 to 200 of about 259,520 (336)

Precision Editing of NLRS Improves Effector Recognition for Enhanced Disease Resistance

Advanced Science, EarlyView.
Precision engineering of plant NLR immune receptors enables rational design of enhanced pathogen resistance through mismatched pairing, domain swapping, and targeted mutagenesis. These approaches achieve multi‐fold expansion in recognition breadth while minimizing autoimmunity risks and fitness penalties.
Vinit Kumar, Vishwa I. P. W. A. Kumara, Pradeepa C. G. Bandaranayake, Dong Yawen, Yang‐Yang Gao, Junfeng Liu, Ge‐Fei Hao, Xiu‐Fang Xin   +7 more
wiley   +1 more source

Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits

Advanced Science, EarlyView.
Xu et al. discovers that switching off auxin transporters in soybean, PIN3a and PIN3b, disrupts auxin flow; this triggers ARF‐STF3/4 signal cascade to activate the nitrate importer NPF2.13 and soil nitrogen acquisition. Multi‐year field trials show edited soybeans maintain yield with higher oil content, offering a potential genetic route to improve ...
Huifang Xu, Shiyu Huang, Jie Wang, Tian Wang, Qingqing Han, Kexin Wu, Zhen Gao, Xiaolei Shi, Tianli Tu, Ming Wang, Laimei Huang, Jiaomei Chen, Yunqi Liu, Yumei Zhang, Guoqiang Lin, Zhichang Chen, Xu Chen   +16 more
wiley   +1 more source

Predicting the effect of CRISPR-Cas9-based epigenome editing. [PDF]

Elife
Batra SS, Cabrera A, Spence JP, Goell J, Anand SS, Hilton IB, Song YS.   +6 more
europepmc   +1 more source

Lipid Droplet‐Localized Spindle Apparatus Coiled‐Coil Protein 1 Regulates Lipid Droplet Distribution

Advanced Science, EarlyView.
SPDL1‐L, a long isoform of SPDL1, is identified as a bona fide lipid droplet‐associated protein that acts as a dynein adaptor to drive perinuclear clustering of lipid droplets. By linking lipid droplets to the microtubule network, SPDL1‐L extends the function of SPDL1 beyond mitosis and contributes to lipid droplet organization and nuclear remodeling ...
Honggang Su, Huimin Pan, Yaqiang Liu, Wei Wang, Yang Jiao, Yumo Hang, Xiahe Huang, Yong E. Zhang, Yuhang Chen, Mei Ding, Xun Huang   +10 more
wiley   +1 more source

Establishment of CRISPR-Cas9-Mediated Gene Editing in the Swimming Crab Portunus trituberculatus. [PDF]

Molecules
Wang X, Chen X, Zhou Y, Zhao Y, Shi C, Li R, Liu L, Mu C, Song W, Wang C.   +9 more
europepmc   +1 more source

Small Nucleolar RNA Snord17 Promotes Self‐Renewal of Intestinal Stem Cells through Yy2 mRNA Export and Tead4 Activation

Advanced Science, EarlyView.
Snord17, through interaction with Thoc3, promotes nuclear export and translation of Yy2 mRNA in Snord17+/+ ISCs. The Yy2 protein subsequently binds the Tead4 promoter to promote its transcription, activating Hippo signaling, which is essential for ISC maintenance.
Peikang Zhang, Yuwei Xu, Yufei Lan, Zhen Xiong, Zhibin Yi, Runyuan Wu, Cunzhen Li, Ying Du, Hui Guo, Yingchi Yang, Zusen Fan   +10 more
wiley   +1 more source

Optimized CRISPR-Cas9 system for efficient engineering of ecDNA in cancer cells. [PDF]

Nucleic Acids Res
Sugimoto Y, Kachi T, Watanabe Y, Kubokawa M, Ogami K, Kawamata M, Yoshino S, Suzuki HI.   +7 more
europepmc   +1 more source

Summary of the CRISPR-Cas9 mutation rates.

Yu-Sheng Tan (21474517), Bao-Tong Mo (13886990), Guo-Cheng Li (11140724), Yu-Ruo Guo (13384770), Jian-Zhen Zhang (21474520), Chen-Zhu Wang (286743)   +5 more
openalex   +1 more source

CRISPR-Cas9 Mediated Gene Editing: A Revolution in Genome Engineering [PDF]

, 2017
Shilpa Sampathi, P Horvath, R Barrangou, J Garneau, M Dupuis, M Villion, D Romero, R Barrangou, Le Cong, F, Ann Ran, David Cox, Shuailiang Lin, Robert Barretto, W Jiang, D Bikard, D Cox, F Zhang, L Marraffini, H Yang, H Wang, C Shivalila, A Cheng, L Shi, J Sander, J Joung, P Mali, K Esvelt, G Church, P Hsu, D Scott, J Weinstein, F Ran, S Konermann, F Ran, P Hsu, C Lin, J Gootenberg, S Konermann, J Kim, D Kim, S Kim, J Kim, I Lokody, Vikram Pattanayak, Steven Lin, John Guilinger, Enbo Ma, Jennifer Doudna, Abba Malina, John Mills, Regina Cencic, Yifei Yan, James Fraser, A Xiao, Z Wang, Y Hu, Y Wu, Z Luo, G Amitai, R Sorek, M Bolukbasi, A Gupta, S Wolfe, T Auer, K Duroure, A De Cian, J Concordet, Del Bene, F, R Deans, D Morgens, A kesli, S Pillay, M Horlbeck, D Du, L Qi, Y Guo, Q Xu, D Canzio, J Shou, J Li   +81 more
openalex   +1 more source

Ferroptosis‐Mediated Hippocampal Neuronal Loss Post‐mTBI: Chromatin Accessibility Profiling and Single‐Nucleus Transcriptomics

Advanced Science, EarlyView.
Hippocampal single ‐nucleus transcriptomes and chromatin accessibility after mild traumatic brain injury reveal dentate granule neuron vulnerability driven by ferroptosis. The c‐Jun–Tmsb4x–Slc2a2 axis modulates lipid peroxidation and iron dysregulation.
Manrui Li, Qiuyun Yang, Shengqiu Qu, Yang Chen, Yang Shen, Yang Xu, Xilong Lin, Yihan Sun, Ying Chen, Meili Lv, Lin Zhang, Zengqiang Yuan, Weibo Liang, Xiameng Chen   +13 more
wiley   +1 more source