Results 171 to 180 of about 45,956 (217)

A type III-associated Cas6 functions as a negative regulator of type I-B CRISPR-Cas system in Thermus thermophilus. [PDF]

Commun Biol
Wei J, Shao Y, Liang Y, Bu X, Zhou W, Liang Y, Li Y.   +6 more
europepmc   +1 more source

Minimizing Off‐Target Effects of CRISPR‐Cas9 With Optimized sgRNA: Evaluation of Efficiency and Specificity in the Tumor Protein 53 (TP53) Region

Biotechnology and Bioengineering, EarlyView.
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) – Cas9‐based genome editing has emerged as a widely used tool across various disciplines, ranging from molecular biology to gene therapy. This revolutionary technology, which enables precise gene editing, represents a significant advancement in biotechnology, opening new frontiers for ...
Ali Mertcan Köse, Monia Ranalli, Drenka Trivanovic, Irina Maslovaric   +3 more
wiley   +1 more source

Glycoengineered Recombinant Alpha1‐Antitrypsin Results in Comparable In Vitro and In Vivo Activities to Human Plasma‐Derived Protein

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Alpha‐1‐antitrypsin (A1AT) is a multifunctional, clinically important, high‐value therapeutic glycoprotein that can be used for the treatment of many diseases, such as A1AT deficiency, diabetes, graft‐versus‐host disease, cystic fibrosis, and various viral infections. Currently, the only U.S. food and drug administration‐approved treatment for
Frances Rocamora, Chen‐Lin Hsieh, Sanne Schoffelen, Johnny Arnsdorf, Eric A. Toth, Abdul S. Yunus, Thomas E. Cleveland IV, Sara Petersen Bjørn, Mina Ying Min Wu, Noel G. McElvaney, Bjørn Gunnar Rude Voldborg, Thomas R. Fuerst, Nathan E. Lewis   +12 more
wiley   +1 more source

Application of CRISPR-Cas System in Human Papillomavirus Detection Using Biosensor Devices and Point-of-Care Technologies. [PDF]

BME Front
He C, Li Y, Liu J, Li Z, Li X, Choi JW, Li H, Liu S, Li CZ.   +8 more
europepmc   +1 more source

Challenges and Future Directives of Synthetic Biology in Engineering Plant–Microbe Partnerships for Sustainable Agriculture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Synthetic biology has recently proven to be a valuable tool for enhancing agriculture even in the face of environmental and biological stresses. Understanding the challenges that militate synthetic biology will assist in ensuring safe, stable, and scalable crop production.
Ben Jesuorsemwen Enagbonma, Rebaona Reaobaka Molefe‐Madlaliso, Alaba Adewole Adebayo, Oghoye Priscilla Oyedoh, Godfrey Okainemen Oribhabor, Oluwaseun Emmanuel Shittu, Olubukola Oluranti Babalola   +6 more
wiley   +1 more source

Internal in-frame translation generates Cas11b, which is important for effective interference in an archaeal CRISPR-Cas system. [PDF]

Front Microbiol
Sailer AL, Brendel J, Chernev A, König S, Bischler T, Gräfenhan T, Urlaub H, Gophna U, Marchfelder A.   +8 more
europepmc   +1 more source

Enhancing the Secretion Systems: Genetic Engineering of Super Bioagents for Effective Plant Disease Control

Biotechnology and Bioengineering, EarlyView.
Genetic engineering (GE) overcomes BCA limits, enabling robust, scalable crop protection. “Super Bioagents” (SBs) use optimized SSs to enhance bioactive molecule delivery. Optimizing SSs enhance precise, sustainable effector deployment for stable disease suppression. SBs use microbiome‐informed design for scalable, next‐gen sustainable crop protection.
Michael Dare Asemoloye
wiley   +1 more source

CRISPR-Cas system positively regulates virulence of Salmonella enterica serovar Typhimurium. [PDF]

Gut Pathog
Sharma N, Das A, Nair AV, Sethi P, Negi VD, Chakravortty D, Marathe SA.   +6 more
europepmc   +1 more source

Pectinase‐Based Bioprocesses for Circular Bioeconomy and Sustainable Industrial Development

Biotechnology and Bioengineering, EarlyView.
This picture demonstrates a unified strategy for sustainable pectinase production, focusing on microbial bioproduction, innovative strategies in enzyme engineering (Protein engineering, rational enzyme design, directed evolution and heterologous expression) and various applications in industry, including food, textiles, pulp and paper, agriculture and ...
Nisha Sharma, Dimple Tanwar, Usha Grewal, Murugesan Mohana Keerthi, Yogesh K. Ahlawat, Nitin Pandey, Chirag G. Makvana   +6 more
wiley   +1 more source

Unlocking Fungal Potential: The CRISPR-Cas System as a Strategy for Secondary Metabolite Discovery. [PDF]

J Fungi (Basel)
Leal K, Rojas E, Madariaga D, Contreras MJ, Nuñez-Montero K, Barrientos L, Goméz-Espinoza O, Iturrieta-González I.   +7 more
europepmc   +1 more source