Results 261 to 270 of about 216,867 (346)

Application of CRISPR-Cas System in Human Papillomavirus Detection Using Biosensor Devices and Point-of-Care Technologies. [PDF]

BME Front
He C, Li Y, Liu J, Li Z, Li X, Choi JW, Li H, Liu S, Li CZ.   +8 more
europepmc   +1 more source

Integrating Human Intestinal Organoids into FDA'S New Approach Methodologies for Drug Discovery

Advanced Science, EarlyView.
Illustration summarizes how human intestinal organoids (HIOs) are becoming transformative in preclinical research. Preclinical drug discovery pipelines often rely on animal models for ADMET studies, even though interspecies ADME gaps, poor external validity, and high attrition rates are common.
Debarun Patra, Ibrahim M. Sayed, Souhrid Mukherjee, Honit Piplani, Aida Habtezion, Michael J. Rosen, Jospeh C. Wu   +6 more
wiley   +1 more source

CRISPR/Cas9‐Based Vanadium MXene‐Free Radical Spatiotemporally Controlled Nanoreactor for Photothermal‐Induced Multi‐Effect Synergistic Antitumor Therapy

Advanced Science, EarlyView.
In this study, a MXene‐based V4C3 nanoplatform, VARH, was constructed, which achieved a photothermal conversion efficiency of 44.21% in the NIR‐II window. This platform integrated photothermal therapy, chemodynamic therapy, and thermodynamic therapy with CRISPR/Cas9‐mediated heat shock protein 90 knock‐out technology.
Zi‐Jian Huang, Feng‐Ming Li, Yi‐Fan Tu, Ke‐Ke Feng, Cheng‐Lei Li, Shi‐Cheng Tian, Yong‐Shan Hu, Jing‐Wei Shao, Zhen‐Hua Liu   +8 more
wiley   +1 more source

Internal in-frame translation generates Cas11b, which is important for effective interference in an archaeal CRISPR-Cas system. [PDF]

Front Microbiol
Sailer AL, Brendel J, Chernev A, König S, Bischler T, Gräfenhan T, Urlaub H, Gophna U, Marchfelder A.   +8 more
europepmc   +1 more source

ALKBH3 m1A Demethylase Deficiency Reduces Alzheimer's Amyloid‐β Pathology

Advanced Science, EarlyView.
This study identifies that ALKBH3‐driven m1A demethylation orchestrates Alzheimer's disease progression by disrupting mitochondrial and synaptic homeostasis. This epitranscriptomic mechanism suppresses PINK1‐mediated mitophagy via m1A erasure, leading to mitochondrial dysfunction, oxidative stress, elevated Aβ production, and impaired microglial ...
Yueyang Li, Sifei Yu, Kaidong Lu, Yujie Zhang, Mingjie Dong, Yan Peng, Liang Xue, Waleed Alam, Yuxuan Shui, Yi Zhou, Wuyunhan Ma, Meng Bao, Peiming Li, Peiyi Luo, Tiezhan Lu, Jiajia Li, Kang Zhang, Yuying Wang, Shuchen Yang, Nuoya Yin, Francesco Faiola, Zilong Gao, Jingfeng Zhou, Fei Zhao, Yali He, Magdalena J. Koziol   +25 more
wiley   +1 more source

The CRISPR-Cas system in Lactiplantibacillus plantarum strains: identification and characterization using a genome mining approach. [PDF]

Front Microbiol
Rostampour M, Panahi B, Masoumi Jahandizi R.   +2 more
europepmc   +1 more source

Nanomedicine Meets Immunotherapy: Advancing Adoptive Cell Therapy with Nanoparticles in the Treatment of Cancer with Sustainability Perspectives

Advanced Science, EarlyView.
This review surveys nanoparticle‐based strategies to enhance adoptive cell therapy, particularly CAR‐T cell approaches, in solid tumor treatment. It describes how nanoparticles can improve tumor immunogenicity and T‐cell infiltration while reducing toxicity, and how they enable in vivo CAR‐T cell generation.
Erica Frostegård, Tatiana Bobrova, Amalie Leinebø, Thibault Leray, Shayamita Ghosh, Lorena García‐Hevia, Chiara Puccinelli, Lorenzo Riccio, Jacopo Sorani, Davide Bonifazi, Julien Caumartin, Emmanuel Donnadieu, Florence Gazeau, Roland Hischier, Else Marit Inderberg, Mónica L. Fanarraga, Maria Loustau, Bernd Nowack, Sébastien Wälchli, Cécilia Ménard‐Moyon   +19 more
wiley   +1 more source

CRISPR-Cas system positively regulates virulence of Salmonella enterica serovar Typhimurium. [PDF]

Gut Pathog
Sharma N, Das A, Nair AV, Sethi P, Negi VD, Chakravortty D, Marathe SA.   +6 more
europepmc   +1 more source

Mass‐Produced and High‐Performance Nanowell Biosensor Fabricated via Semiconductor Manufacturing for Rapid and Accurate COVID‐19 Diagnosis in the Clinical Field

Advanced Science, EarlyView.
A semiconductor‐fabricated nanowell biosensor enables rapid, scalable, and highly reproducible detection of SARS‐CoV‐2 antigens from nasal swabs within ∼10 minutes. Clinical validation in 249 retrospective and 243 prospective patient samples demonstrates high sensitivity and specificity, minimal cross‐reactivity, and robust batch‐to‐batch ...
Yoo Min Park, Zahra Rezaei, Nam Ho Bae, Donggee Rho, Da‐Seul Kim, EunYoung Go, YoungTae Seo, Seok Jae Lee, Won Chan Seo, Luke P. Lee, HeaYeon Lee, Su Ryon Shin   +11 more
wiley   +1 more source

Unlocking Fungal Potential: The CRISPR-Cas System as a Strategy for Secondary Metabolite Discovery. [PDF]

J Fungi (Basel)
Leal K, Rojas E, Madariaga D, Contreras MJ, Nuñez-Montero K, Barrientos L, Goméz-Espinoza O, Iturrieta-González I.   +7 more
europepmc   +1 more source