Results 11 to 20 of about 64,601 (279)

Phylogenetic Distribution of CRISPR-Cas Systems in Staphylococcus lugdunensis

Microbiology Spectrum, 2021
Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) genes (CRISPR-Cas) are present in many bacterial genomes with functions beyond adaptive immunity.
Cheng-Yen Kao, Jang-Jih Lu, Lee-Chung Lin, Hsiao-Chi Lin, Shih-Cheng Chang   +4 more
doaj   +1 more source

CRISPR-Cas systems feature and targeting phages diversity in Lacticaseibacillus rhamnosus strains

Frontiers in Microbiology, 2023
One of the most important adaptive immune systems in bacteria against phages is clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (CAS) genes.
Bahman Panahi, Behnaz Dehganzad, Yousef Nami   +2 more
doaj   +1 more source

Nonviral Delivery of CRISPR/Cas Systems in mRNA Format

Advanced NanoBiomed Research, 2022
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas) genome editing system provides a powerful toolbox for genetic engineering.
Zhen Liu, Zhenghua Li, Bin Li
doaj   +1 more source

An Endogenous Staphylococcus aureus CRISPR-Cas System Limits Phage Proliferation and Is Efficiently Excised from the Genome as Part of the SCCmec Cassette

Microbiology Spectrum, 2023
CRISPR-Cas is an adaptive immune system that allows bacteria to inactivate mobile genetic elements. Approximately 50% of bacteria harbor CRISPR-Cas; however, in the human pathogen Staphylococcus aureus, CRISPR-Cas loci are less common and often studied ...
Kasper Mikkelsen, Janine Zara Bowring, Yong Kai Ng, Frida Svanberg Frisinger, Julie Kjærsgaard Maglegaard, Qiuchun Li, Raphael N. Sieber, Andreas Petersen, Paal Skytt Andersen, Jakob T. Rostøl, Nina Molin Høyland-Kroghsbo, Hanne Ingmer   +11 more
doaj   +1 more source

Type I CRISPR-Cas-mediated microbial gene editing and regulation

AIMS Microbiology, 2023
There are six major types of CRISPR-Cas systems that provide adaptive immunity in bacteria and archaea against invasive genetic elements. The discovery of CRISPR-Cas systems has revolutionized the field of genetics in many organisms.
Zeling Xu , Shuzhen Chen, Weiyan Wu, Yongqi Wen, Huiluo Cao   +4 more
doaj   +1 more source

Application of different types of CRISPR/Cas-based systems in bacteria

Microbial Cell Factories, 2020
As important genome editing tools, CRISPR/Cas systems, especially those based on type II Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria.
Zhenquan Liu, Huina Dong, Yali Cui, Lina Cong, Dawei Zhang   +4 more
doaj   +1 more source

Optimization of CRISPR–Cas system for clinical cancer therapy

Bioengineering & Translational Medicine, 2023
Cancer is a genetic disease caused by alterations in genome and epigenome and is one of the leading causes for death worldwide. The exploration of disease development and therapeutic strategies at the genetic level have become the key to the treatment of
Xiang Meng, Tian‐gang Wu, Qiu‐yue Lou, Kai‐yuan Niu, Lei Jiang, Qing‐zhong Xiao, Tao Xu, Lei Zhang   +7 more
doaj   +1 more source

A global comprehensive study of the distribution of type I-E and type I-E* CRISPR-Cas systems in Klebsiella pneumoniae

Indian Journal of Community Medicine, 2023
Background: The CRISPR-Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems are the short DNA sequences and RNA-dependent nuclease involved in the adaptive immunity in bacteria and archaea. The type of CRISPR-
Anand Babu Kannadasan, Venil Naranan Sumantran, Rama Vaidyanathan   +2 more
doaj   +1 more source

Characterization of CRISPR-Cas Systems in Serratia marcescens Isolated from Rhynchophorus ferrugineus (Olivier, 1790) (Coleoptera: Curculionidae) [PDF]

, 2019
The CRISPR-Cas adaptive immune system has been attracting increasing scientific interest for biological functions and biotechnological applications. Data on the Serratia marcescens system are scarce.
Calia, Carla, Dionisi Anna, Maria, D’Addabbo, Pietro, Oliva, Marta, Pazzani, Carlo, Porcelli, Francesco, Roberto, Roberta, Scrascia, Maria   +7 more
core   +1 more source

Clades of huge phages from across Earth's ecosystems. [PDF]

, 2020
Bacteriophages typically have small genomes1 and depend on their bacterial hosts for replication2. Here we sequenced DNA from diverse ecosystems and found hundreds of phage genomes with lengths of more than 200 kilobases (kb), including a genome of ...
Al-Shayeb, Basem, Amano, Yuki, Amundson, Ronald, Anantharaman, Karthik, Banfield, Jillian F, Borton, Mikayla A, Bouma-Gregson, Keith, Brooks, Brandon, Castelle, Cindy J, Cate, Jamie HD, Chen, Lin-Xing, Devoto, Audra, Doudna, Jennifer A, Farag, Ibrahim F, Finstad, Kari, Goltsman, Daniela SA, Harrison, Sue, He, Christine, Jaffe, Alexander L, Kantor, Rose, Keren, Ray, Lane, Katherine R, Lavy, Adi, Lehours, Anne-Catherine, Lei, Shufei, Li, Wen-Jun, Matheus-Carnevali, Paula, Morowitz, Michael, Munk, Patrick, Méheust, Raphaël, Nelson, Tara C, Olm, Matthew R, Power, Mary E, Probst, Alexander J, Relman, David A, Sachdeva, Rohan, Santini, Joanne M, Sharrar, Allison, Sun, Christine, Thomas, Alex, Tringe, Susannah G, Ward, Fred, Warren, Lesley, Wrighton, Kelly, Zhou, Jinglie   +44 more
core   +2 more sources