Results 21 to 30 of about 38,793 (285)
Type I CRISPR-Cas-mediated microbial gene editing and regulation
AIMS Microbiology, 2023 There are six major types of CRISPR-Cas systems that provide adaptive immunity in bacteria and archaea against invasive genetic elements. The discovery of CRISPR-Cas systems has revolutionized the field of genetics in many organisms.
Zeling Xu +4 more
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CRISPR-Cas systems in multicellular cyanobacteria [PDF]
RNA Biology, 2018 Novel CRISPR-Cas systems possess substantial potential for genome editing and manipulation of gene expression. The types and numbers of CRISPR-Cas systems vary substantially between different organisms. Some filamentous cyanobacteria harbor > 40 different putative CRISPR repeat-spacer cassettes, while the number of cas gene instances is much lower ...
Shengwei Hou +6 more
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Microbiology Spectrum, 2023 CRISPR-Cas is an adaptive immune system that allows bacteria to inactivate mobile genetic elements. Approximately 50% of bacteria harbor CRISPR-Cas; however, in the human pathogen Staphylococcus aureus, CRISPR-Cas loci are less common and often studied ...
Kasper Mikkelsen +11 more
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Application of different types of CRISPR/Cas-based systems in bacteria
Microbial Cell Factories, 2020 As important genome editing tools, CRISPR/Cas systems, especially those based on type II Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria.
Zhenquan Liu +4 more
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Optimization of CRISPR–Cas system for clinical cancer therapy
Bioengineering & Translational Medicine, 2023 Cancer is a genetic disease caused by alterations in genome and epigenome and is one of the leading causes for death worldwide. The exploration of disease development and therapeutic strategies at the genetic level have become the key to the treatment of
Xiang Meng +7 more
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CRISPR-Cas systems in Proteus mirabilis
Infection, Genetics and Evolution, 2021 The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a bacterial defense mechanism against bacteriophages composed of two different parts: the CRISPR array and the Cas genes. The spacer acquisition is done by the adaptation module consisting of the hallmark Cas1 Cas2 proteins, which inserts new spacers into the CRISPR array.
Mahnaz Shafaei, Fallah +3 more
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Using CRISPR-Cas systems as antimicrobials [PDF]
Current Opinion in Microbiology, 2017 Although CRISPR-Cas systems naturally evolved to provide adaptive immunity in bacteria and archaea, Cas nucleases can be co-opted to target chromosomal sequences rather than invasive genetic elements. Although genome editing is the primary outcome of self-targeting using CRISPR-based technologies in eukaryotes, self-targeting by CRISPR is typically ...
Bikard, David, Barrangou, Rodolphe
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Indian Journal of Community Medicine, 2023 Background: The CRISPR-Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems are the short DNA sequences and RNA-dependent nuclease involved in the adaptive immunity in bacteria and archaea. The type of CRISPR-
Anand Babu Kannadasan +2 more
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Viral Diversity Threshold for Adaptive Immunity in Prokaryotes
mBio, 2012 Bacteria and archaea face continual onslaughts of rapidly diversifying viruses and plasmids. Many prokaryotes maintain adaptive immune systems known as clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (Cas ...
Ariel D. Weinberger +4 more
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Research advances on pathogenic nucleic acid detection technology based on CRIPSR/Cas system
浙江大学学报. 农业与生命科学版, 2023 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) (CRISPR/Cas) system, an ancient bacterial and archaeal immune system, has rapidly developed into a popular gene-editing tool, which largely promotes the ...
LI Hongzhao +4 more
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