Results 21 to 30 of about 58,663 (324)

CRISPR-Cas systems in multicellular cyanobacteria [PDF]

open access: bronzeRNA Biology, 2018
Novel CRISPR-Cas systems possess substantial potential for genome editing and manipulation of gene expression. The types and numbers of CRISPR-Cas systems vary substantially between different organisms. Some filamentous cyanobacteria harbor > 40 different putative CRISPR repeat-spacer cassettes, while the number of cas gene instances is much lower ...
Shengwei Hou   +6 more
openalex   +6 more sources

CRISPR-Cas Systems in Streptococci [PDF]

open access: yesCurrent Issues in Molecular Biology, 2019
Streptococci are one of the most important and common constituents of the host's microbiota and can colonize and live in the upper respiratory and urogenital tract of humans and animals. The CRISPR-Cas systems (i.e., clustered regularly interspaced short palindromic repeat, with CRISPR-associated proteins) found in bacteria and archaea provide sequence-
Boyu Tang   +7 more
openaire   +3 more sources

The CRISPR-Cas system in Enterobacteriaceae [PDF]

open access: yesPathogens and Disease, 2018
In nature, microorganisms are constantly exposed to multiple viral infections and thus have developed many strategies to survive phage attack and invasion by foreign DNA. One of such strategies is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) bacterial immunological system.
Sonia Dávila   +4 more
openaire   +3 more sources

Using CRISPR-Cas systems as antimicrobials [PDF]

open access: yesCurrent Opinion in Microbiology, 2017
Although CRISPR-Cas systems naturally evolved to provide adaptive immunity in bacteria and archaea, Cas nucleases can be co-opted to target chromosomal sequences rather than invasive genetic elements. Although genome editing is the primary outcome of self-targeting using CRISPR-based technologies in eukaryotes, self-targeting by CRISPR is typically ...
Bikard, David, Barrangou, Rodolphe
openaire   +4 more sources

Evolution and classification of the CRISPR–Cas systems [PDF]

open access: yesNature Reviews Microbiology, 2011
The CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) modules are adaptive immunity systems that are present in many archaea and bacteria. These defence systems are encoded by operons that have an extraordinarily diverse architecture and a high rate of evolution for both the cas genes and the unique ...
Yuri I. Wolf   +11 more
openaire   +4 more sources

Therapeutic Applications of the CRISPR-Cas System

open access: yesBioengineering, 2022
The clustered regularly interspaced palindromic repeat (CRISPR)-Cas system has revolutionized genetic engineering due to its simplicity, stability, and precision since its discovery. This technology is utilized in a variety of fields, from basic research in medicine and biology to medical diagnosis and treatment, and its potential is unbounded as new ...
Kyungmin Kang   +3 more
openaire   +3 more sources

Nonviral Delivery of CRISPR/Cas Systems in mRNA Format

open access: yesAdvanced NanoBiomed Research, 2022
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas) genome editing system provides a powerful toolbox for genetic engineering.
Zhen Liu, Zhenghua Li, Bin Li
doaj   +1 more source

Type I CRISPR-Cas-mediated microbial gene editing and regulation

open access: yesAIMS Microbiology, 2023
There are six major types of CRISPR-Cas systems that provide adaptive immunity in bacteria and archaea against invasive genetic elements. The discovery of CRISPR-Cas systems has revolutionized the field of genetics in many organisms.
Zeling Xu   +4 more
doaj   +1 more source

The structural biology of CRISPR-Cas systems [PDF]

open access: yesCurrent Opinion in Structural Biology, 2015
Prokaryotic CRISPR-Cas genomic loci encode RNA-mediated adaptive immune systems that bear some functional similarities with eukaryotic RNA interference. Acquired and heritable immunity against bacteriophage and plasmids begins with integration of ∼30 base pair foreign DNA sequences into the host genome.
Jiang, Fuguo, Doudna, Jennifer A
openaire   +5 more sources

An Endogenous Staphylococcus aureus CRISPR-Cas System Limits Phage Proliferation and Is Efficiently Excised from the Genome as Part of the SCCmec Cassette

open access: yesMicrobiology Spectrum, 2023
CRISPR-Cas is an adaptive immune system that allows bacteria to inactivate mobile genetic elements. Approximately 50% of bacteria harbor CRISPR-Cas; however, in the human pathogen Staphylococcus aureus, CRISPR-Cas loci are less common and often studied ...
Kasper Mikkelsen   +11 more
doaj   +1 more source

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