Results 41 to 50 of about 38,793 (285)

Adaptation by Type III CRISPR-Cas Systems: Breakthrough Findings and Open Questions

Frontiers in Microbiology, 2022
CRISPR-Cas systems acquire heritable defense memory against invading nucleic acids through adaptation. Type III CRISPR-Cas systems have unique and intriguing features of defense and are important in method development for Genetics research. We started to
Xinfu Zhang, Xinfu Zhang, Xinmin An
doaj   +1 more source

Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems

Biology Direct, 2011
Background The CRISPR-Cas adaptive immunity systems that are present in most Archaea and many Bacteria function by incorporating fragments of alien genomes into specific genomic loci, transcribing the inserts and using the transcripts as guide RNAs to ...
Wolf Yuri I, Aravind L, Makarova Kira S, Koonin Eugene V   +3 more
doaj   +1 more source

Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus

Frontiers in Microbiology, 2023
Streptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S.
Richard Bauer, Dorina Haider, Aline Grempels, Rebecca Roscher, Stefanie Mauerer, Barbara Spellerberg   +5 more
doaj   +1 more source

SnapShot: Class 1 CRISPR-Cas Systems

Cell, 2017
Class 1 CRISPR-Cas systems are characterized by effector modules consisting of multiple subunits. Class 1 systems comprise about 90% of all CRISPR-Cas loci identified in bacteria and archaea and can target both DNA and RNA.
Kira S, Makarova, Feng, Zhang, Eugene V, Koonin   +2 more
openaire   +2 more sources

Role of CRISPR-Cas systems and anti-CRISPR proteins in bacterial antibiotic resistance. [PDF]

Heliyon
Kadkhoda H, Gholizadeh P, Samadi Kafil H, Ghotaslou R, Pirzadeh T, Ahangarzadeh Rezaee M, Nabizadeh E, Feizi H, Aghazadeh M.   +8 more
europepmc   +2 more sources

The structural biology of CRISPR-Cas systems [PDF]

Current Opinion in Structural Biology, 2015
Prokaryotic CRISPR-Cas genomic loci encode RNA-mediated adaptive immune systems that bear some functional similarities with eukaryotic RNA interference. Acquired and heritable immunity against bacteriophage and plasmids begins with integration of ∼30 base pair foreign DNA sequences into the host genome.
Jiang, Fuguo, Doudna, Jennifer A
openaire   +5 more sources

CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges

Heliyon
CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various CRISPR/Cas systems such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 and CRISPR/Cas3 utilize different mechanisms to detect or ...
Jian Zhou, Zhuo Li, Joshua Seun Olajide, Gang Wang   +3 more
doaj   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Phylogenetic Distribution of CRISPR-Cas Systems in Antibiotic-Resistant Pseudomonas aeruginosa

mBio, 2015
Pseudomonas aeruginosa is an antibiotic-refractory pathogen with a large genome and extensive genotypic diversity. Historically, P. aeruginosa has been a major model system for understanding the molecular mechanisms underlying type I clustered regularly ...
Alex van Belkum, Leah B. Soriaga, Matthew C. LaFave, Srividya Akella, Jean-Baptiste Veyrieras, E. Magda Barbu, Dee Shortridge, Bernadette Blanc, Gregory Hannum, Gilles Zambardi, Kristofer Miller, Mark C. Enright, Nathalie Mugnier, Daniel Brami, Stéphane Schicklin, Martina Felderman, Ariel S. Schwartz, Toby H. Richardson, Todd C. Peterson, Bolyn Hubby, Kyle C. Cady   +20 more
doaj   +1 more source

CRISPR-Cas-Based Engineering of Probiotics

BioDesign Research, 2023
Probiotics are the treasure of the microbiology fields. They have been widely used in the food industry, clinical treatment, and other fields. The equivocal health-promoting effects and the unknown action mechanism were the largest obstacles for further ...
Ling Liu, Shimaa Elsayed Helal, Nan Peng
doaj   +1 more source