Results 51 to 60 of about 58,663 (324)

CRISPR-Cas-Docker: web-based in silico docking and machine learning-based classification of crRNAs with Cas proteins

BMC Bioinformatics, 2023
Background CRISPR-Cas-Docker is a web server for in silico docking experiments with CRISPR RNAs (crRNAs) and Cas proteins. This web server aims at providing experimentalists with the optimal crRNA-Cas pair predicted computationally when prokaryotic ...
Ho-min Park, Jongbum Won, Yunseol Park, Esla Timothy Anzaku, Joris Vankerschaver, Arnout Van Messem, Wesley De Neve, Hyunjin Shim   +7 more
doaj   +1 more source

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR-Cas effector complexes [PDF]

, 2017
Prokaryotes encode various host defense systems that provide protection against mobile genetic elements. Restriction-modification (R-M) and CRISPR-Cas systems mediate host defense by sequence specific targeting of invasive DNA.
Anders, C., Brouns, S.J.J., Dickman, M.J., Houkes, J., Jinek, M., Kunne, T., Lochs, S.J.A., Mohanraju, P., Swarts, D.C., van der Oost, J., Vlot, M., Zheng, P.   +11 more
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Adaptation by Type III CRISPR-Cas Systems: Breakthrough Findings and Open Questions

Frontiers in Microbiology, 2022
CRISPR-Cas systems acquire heritable defense memory against invading nucleic acids through adaptation. Type III CRISPR-Cas systems have unique and intriguing features of defense and are important in method development for Genetics research. We started to
Xinfu Zhang, Xinfu Zhang, Xinmin An
doaj   +1 more source

Drug Inducible CRISPR/Cas Systems

Computational and Structural Biotechnology Journal, 2019
Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have been employed as a powerful versatile technology for programmable gene editing, transcriptional modulation, epigenetic modulation, and genome labeling, etc.
Yu Wang, Li Chen, Ju Zhang, Jingfang Zhang   +3 more
openaire   +4 more sources

An updated evolutionary classification of CRISPR–Cas systems [PDF]

Nature Reviews Microbiology, 2015
The evolution of CRISPR-cas loci, which encode adaptive immune systems in archaea and bacteria, involves rapid changes, in particular numerous rearrangements of the locus architecture and horizontal transfer of complete loci or individual modules. These dynamics complicate straightforward phylogenetic classification, but here we present an approach ...
Kira S. Makarova, Rebecca M. Terns, Malcolm F. White, Stan J. J. Brouns, Rodolphe Barrangou, Philippe Horvath, Alexander F. Yakunin, Francisco J. M. Mojica, Daniel H. Haft, Roger A. Garrett, Shiraz A. Shah, Eugene V. Koonin, Fabrizio Costa, Emmanuelle Charpentier, Sylvain Moineau, Omer S. Alkhnbashi, Michael P. Terns, Yuri I. Wolf, John van der Oost, Sita J. Saunders, Rolf Backofen   +20 more
openaire   +4 more sources

Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells. [PDF]

, 2019
Repurposed CRISPR-Cas molecules provide a useful tool set for broad applications of genomic editing and regulation of gene expression in prokaryotes and eukaryotes. Recent discovery of phage-derived proteins, anti-CRISPRs, which serve to abrogate natural
Abbott, Timothy R, Bondy-Denomy, Joseph, Carter, Matthew A, Chavez, Michael, Dominguez, Antonia A, Gao, Yuchen, Kipniss, Nathan H, La Russa, Marie, Lau, Matthew B, Nakamura, Muneaki, Qi, Lei S, Smolke, Christina D, Srinivasan, Prashanth, Xu, Xiaoshu, Zhao, Dehua   +14 more
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CRISPR-Cas in Acinetobacter baumannii Contributes to Antibiotic Susceptibility by Targeting Endogenous AbaI

Microbiology Spectrum, 2022
Acinetobacter baumannii is a well-known human opportunistic pathogen in nosocomial infections, and the emergence of multidrug-resistant Acinetobacter baumannii has become a complex problem for clinical anti-infective treatments.
Yuhang Wang, Jie Yang, Xiaoli Sun, Mengying Li, Pengyu Zhang, Zhongtian Zhu, Hongmei Jiao, Tingting Guo, Guocai Li   +8 more
doaj   +1 more source

Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS [PDF]

, 2016
Background: CRISPR-Cas systems provide adaptive immunity to mobile genetic elements in prokaryotes. In many bacteria, including E. coli, a specialized ribonucleoprotein complex called Cascade enacts immunity by “an interference reaction" between CRISPR ...
A Shinkai, B Gong, B Sonden, B Wiedenheft, B Wiedenheft, C Almendros, C Xue, CD Yang, D Bhaya, DC Swarts, DG Sashital, E Semenova, Edward L. Bolt, ER Westra, ER Westra, ER Westra, ER Westra, FJ Mojica, G Nonaka, H Deveau, H Zhao, I Ivančić-Baće, I Ivančić-Baće, I Yosef, Ivana Ivančić-Baće, J van der Oost, JH Miller, JT Wade, K Pougach, KA Datsenko, KA Datsenko, Kristina Majsec, M Rutkauskas, MD Szczelkun, ML Hochstrasser, MM Jore, N Beloglazova, PC Fineran, R Sorek, RB Vercoe, RN Jackson, S Mulepati, S Mulepati, S Redding, SJ Brouns, T Sinkunas, T Sinkunas, TR Blosser, Y Huo, Ü Pul   +49 more
core   +3 more sources

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems [PDF]

, 2017
Microbial CRISPR-Cas systems are divided into Class 1, with multisubunit effector complexes, and Class 2, with single protein effectors. Currently, only two Class 2 effectors, Cas9 and Cpf1, are known.
Abudayyeh, Omar Osama, Gootenberg, Jonathan S, Joung, Julia, Konermann, Silvana M, Koonin, Eugene V., Makarova, Kira S., Minakhin, Leonid, Semenova, Ekaterina, Severinov, Konstantin, Shmakov, Sergey, Wolf, Yuri I., Zhang, Feng   +11 more
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Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus

Frontiers in Microbiology, 2023
Streptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S.
Richard Bauer, Dorina Haider, Aline Grempels, Rebecca Roscher, Stefanie Mauerer, Barbara Spellerberg   +5 more
doaj   +1 more source