Results 51 to 60 of about 717,978 (310)

Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus

Frontiers in Microbiology, 2023
Streptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S.
Richard Bauer, Dorina Haider, Aline Grempels, Rebecca Roscher, Stefanie Mauerer, Barbara Spellerberg   +5 more
doaj   +1 more source

Annotation and Classification of CRISPR-Cas Systems [PDF]

, 2015
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas (CRISPR-associated proteins) is a prokaryotic adaptive immune system that is represented in most archaea and many bacteria. Among the currently known prokaryotic defense systems, the CRISPR-Cas genomic loci show unprecedented complexity and diversity.
Eugene V. Koonin, Kira S. Makarova
openaire   +2 more sources

Genetically Engineering the Nervous System with CRISPR-Cas [PDF]

eneuro, 2020
The multitude of neuronal subtypes and extensive interconnectivity of the mammalian brain presents a substantial challenge to those seeking to decipher its functions. While the molecular mechanisms of several neuronal functions remain poorly characterized, advances in next-generation sequencing (NGS) and gene-editing technology have begun to close this
Alfredo Sandoval, Hajira Elahi, Jonathan E. Ploski   +2 more
openaire   +3 more sources

The Application of the CRISPR-Cas System in Antibiotic Resistance

Infection and Drug Resistance, 2022
The emergence and global epidemic of antimicrobial resistance (AMR) poses a serious threat to global public health in recent years. AMR genes are shared between bacterial pathogens mainly via horizontal gene transfer (HGT) on mobile genetic elements (MGEs), thereby accelerating the spread of antimicrobial resistance (AMR) and increasing the burden of ...
Shuan Tao, Huimin Chen, Na Li, Wei Liang
openaire   +4 more sources

Phylogenetic Distribution of CRISPR-Cas Systems in Antibiotic-Resistant Pseudomonas aeruginosa

mBio, 2015
Pseudomonas aeruginosa is an antibiotic-refractory pathogen with a large genome and extensive genotypic diversity. Historically, P. aeruginosa has been a major model system for understanding the molecular mechanisms underlying type I clustered regularly ...
Alex van Belkum, Leah B. Soriaga, Matthew C. LaFave, Srividya Akella, Jean-Baptiste Veyrieras, E. Magda Barbu, Dee Shortridge, Bernadette Blanc, Gregory Hannum, Gilles Zambardi, Kristofer Miller, Mark C. Enright, Nathalie Mugnier, Daniel Brami, Stéphane Schicklin, Martina Felderman, Ariel S. Schwartz, Toby H. Richardson, Todd C. Peterson, Bolyn Hubby, Kyle C. Cady   +20 more
doaj   +1 more source

CRISPR-Cas-Based Engineering of Probiotics

BioDesign Research, 2023
Probiotics are the treasure of the microbiology fields. They have been widely used in the food industry, clinical treatment, and other fields. The equivocal health-promoting effects and the unknown action mechanism were the largest obstacles for further ...
Ling Liu, Shimaa Elsayed Helal, Nan Peng
doaj   +1 more source

RNA-Targeting CRISPR–Cas Systems and Their Applications [PDF]

International Journal of Molecular Sciences, 2020
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR–Cas systems have been used as a backbone for the development of potent research tools, with Cas9 being the most widespread.
Michal Burmistrz, Kamil Krakowski, Agata Krawczyk-Balska   +2 more
openaire   +3 more sources

Substrate Generation for Endonucleases of CRISPR/Cas Systems [PDF]

Journal of Visualized Experiments, 2012
The interaction of viruses and their prokaryotic hosts shaped the evolution of bacterial and archaeal life. Prokaryotes developed several strategies to evade viral attacks that include restriction modification, abortive infection and CRISPR/Cas systems. These adaptive immune systems found in many Bacteria and most Archaea consist of clustered regularly
Judith Zoephel, Hagen Richter, Srivatsa Dwarakanath, André Plagens, Lennart Randau   +4 more
openaire   +3 more sources

TRPM4 contributes to cell death in prostate cancer tumor spheroids, and to extravasation and metastasis in a zebrafish xenograft model system

Molecular Oncology, EarlyView.
Transient receptor potential melastatin‐4 (TRPM4) is overexpressed in prostate cancer (PCa). Knockout of TRPM4 resulted in reduced PCa tumor spheroid size and decreased PCa tumor spheroid outgrowth. In addition, lack of TRPM4 increased cell death in PCa tumor spheroids.
Florian Bochen, Saurav Subedi, Federico La Manna, Sofia Jarrin, Irida Papapostolou, Marianna Kruithof‐de Julio, Christine Peinelt   +6 more
wiley   +1 more source

PAM-repeat associations and spacer selection preferences in single and co-occurring CRISPR-Cas systems

Genome Biology, 2021
Background The adaptive CRISPR-Cas immune system stores sequences from past invaders as spacers in CRISPR arrays and thereby provides direct evidence that links invaders to hosts.
Jochem N. A. Vink, Jan H. L. Baijens, Stan J. J. Brouns   +2 more
doaj   +1 more source