Results 11 to 20 of about 321,595 (358)
Current Protocols in Stem Cell Biology, 2016 AbstractCRISPR/Cas9‐gene editing has emerged as a revolutionary technology to easily modify specific genomic loci by designing complementary sgRNA sequences and introducing these into cells along with Cas9. Self‐cloning CRISPR/Cas9 (scCRISPR) uses a self‐cleaving palindromic sgRNA plasmid (sgPal) that recombines with short PCR‐amplified site‐specific ...
Arbab, M., Sherwood, R. I.
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CRISPR-Cas Systems for Detection of Nucleic Acids: PCR Empire now has a Vigorous Rival! [PDF]
Translational Research in Urology, 2021 Rapid and accurate detection of nucleic acids is essential in many fields of modern biology and medical diagnosis of pathogens. PCR has some disadvantages for performing this task, and therefore demand for an alternative is highly appreciated.
Roham Deyhimfar
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RNA Biology, 2013 CRISPR loci consist of an array of short repeats separated by spacer sequences that match the genome of viruses and plasmids that infect prokaryotes. Transcription of the CRISPR array generates small antisense RNAs that mediate immunity against these invaders.
Inbal, Maniv +2 more
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CRISPR-Cas12–based detection of SARS-CoV-2
Nature Biotechnology, 2020 An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic.
James P. Broughton +19 more
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CRISPR-based diagnostics [PDF]
Nature Biomedical Engineering, 2021 The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic acids are among the most sensitive and specific, yet most such assays require costly equipment and trained personnel.
Michael M. Kaminski +4 more
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Improved vectors and genome-wide libraries for CRISPR screening
Nature Methods, 2014 Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)–associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and ...
Neville E. Sanjana +2 more
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CRISPR/Cas9 therapeutics: progress and prospects
Signal Transduction and Targeted Therapy, 2023 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases by permanently correcting deleterious base mutations or disrupting disease-
Tianxiang Li +9 more
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Book Review: “The New Microbiology: From Microbiomes to CRISPR”
Annals of Clinical Microbiology, 2022 At the time when owing to COVID-19 pandemic, the public is more interested in infectious diseases and pathogens than in any other period, a book titled “The New Microbiology: From Microbiomes to CRISPR” by the renowned microbiologist Pascale Cossart was ...
Jung Oak Kang
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Off-target effects in CRISPR/Cas9 gene editing
Frontiers in Bioengineering and Biotechnology, 2023 Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient ...
Congting Guo +3 more
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A Unique B-Family DNA Polymerase Facilitating Error-Prone DNA Damage Tolerance in Crenarchaeota
Frontiers in Microbiology, 2020 Sulfolobus islandicus codes for four DNA polymerases: three are of the B-family (Dpo1, Dpo2, and Dpo3), and one is of the Y-family (Dpo4). Western analysis revealed that among the four polymerases, only Dpo2 exhibited DNA damage-inducible expression.
Xu Feng +7 more
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