Results 21 to 30 of about 277,742 (368)
Multiplex Genome Engineering Using CRISPR/Cas Systems
Science, 2013 Genome Editing Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound
Le Cong +10 more
semanticscholar +1 more source
CRISPR-Cas12–based detection of SARS-CoV-2
Nature Biotechnology, 2020 An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic.
James P. Broughton +19 more
semanticscholar +1 more source
Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
Nature Communications, 2023 CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past decade and holds great promise for the treatment of human genetic diseases.
Jianli Tao, D. E. Bauer, R. Chiarle
semanticscholar +1 more source
Book Review: “The New Microbiology: From Microbiomes to CRISPR”
Annals of Clinical Microbiology, 2022 At the time when owing to COVID-19 pandemic, the public is more interested in infectious diseases and pathogens than in any other period, a book titled “The New Microbiology: From Microbiomes to CRISPR” by the renowned microbiologist Pascale Cossart was ...
Jung Oak Kang
doaj +1 more source
Improved vectors and genome-wide libraries for CRISPR screening
Nature Methods, 2014 Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)–associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and ...
Neville E. Sanjana +2 more
semanticscholar +1 more source
CRISPR: A Screener’s Guide [PDF]
SLAS Discovery, 2020 The discovery of CRISPR-Cas9 systems has fueled a rapid expansion of gene editing adoption and has impacted pharmaceutical and biotechnology research substantially. Here, gene editing is used at an industrial scale to identify and validate new biological targets for precision medicines, with functional genomic screening having an increasingly important
Carlos le Sage +2 more
openaire +3 more sources
Funding CRISPR: Understanding the role of government and philanthropic institutions in supporting academic research within the CRISPR innovation system [PDF]
Quantitative Science Studies (2022) 3 (2): 443-456, 2020 CRISPR/Cas has the potential to revolutionize medicine, agriculture, and biology. Understanding the trajectory of CRISPR research, how it is influenced and who pays for it, is an essential research policy question. We use a combination of methods to map, via quantitative content analysis of CRISPR papers, the research funding profile of major ...
arxiv +1 more source
A Unique B-Family DNA Polymerase Facilitating Error-Prone DNA Damage Tolerance in Crenarchaeota
Frontiers in Microbiology, 2020 Sulfolobus islandicus codes for four DNA polymerases: three are of the B-family (Dpo1, Dpo2, and Dpo3), and one is of the Y-family (Dpo4). Western analysis revealed that among the four polymerases, only Dpo2 exhibited DNA damage-inducible expression.
Xu Feng +7 more
doaj +1 more source
Current trends and new methods of detection of SARS-CoV-2 infection [PDF]
Cellular, Molecular and Biomedical Reports, 2022 SARS-CoV- 2 is the causative agent of the global pandemic, also known as Covid-19. This virus belongs to a group of coronaviruses and has affected more than ten million people across the globe, causing nearly half a million deaths worldwide. The pandemic
Mohammad Mohammadi +2 more
doaj +1 more source
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Science, 2018 Taking CRISPR technology further CRISPR techniques are allowing the development of technologies for nucleic acid detection (see the Perspective by Chertow).
Janice S. Chen +6 more
semanticscholar +1 more source