A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]
, 2015 Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G. +7 more
core +2 more sources
Characterization of CRISPR-Cas Systems in Serratia marcescens Isolated from Rhynchophorus ferrugineus (Olivier, 1790) (Coleoptera: Curculionidae) [PDF]
, 2019 The CRISPR-Cas adaptive immune system has been attracting increasing scientific interest for biological functions and biotechnological applications. Data on the Serratia marcescens system are scarce.
Calia, Carla +7 more
core +1 more source
Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]
, 2020 The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes +5 more
core +1 more source
Improved vectors and genome-wide libraries for CRISPR screening
Nature Methods, 2014 Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)–associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and ...
Neville E. Sanjana +2 more
semanticscholar +1 more source
A review on genome editing by CRISPR-Cas9 technique for cancer treatment [PDF]
World Cancer Research Journal, 2020 In this review, we summarize CRISPR-Cas9 system-based gene modification for the therapeutic treatment of cancer. Cancer is a group of diseases involving anomalous cell growth with the potential to invade or blow out to other parts of the body, which is ...
M. Niuz Morshed Khan +3 more
doaj +1 more source
Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections. [PDF]
, 2018 The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein⁻Barr virus, cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus.
Chen, Yuan-Chuan +3 more
core +2 more sources
RNA Biology, 2013 CRISPR loci consist of an array of short repeats separated by spacer sequences that match the genome of viruses and plasmids that infect prokaryotes. Transcription of the CRISPR array generates small antisense RNAs that mediate immunity against these invaders.
Asma Hatoum-Aslan +2 more
openaire +3 more sources
CRISPR: A Screener’s Guide [PDF]
SLAS Discovery, 2020 The discovery of CRISPR-Cas9 systems has fueled a rapid expansion of gene editing adoption and has impacted pharmaceutical and biotechnology research substantially. Here, gene editing is used at an industrial scale to identify and validate new biological targets for precision medicines, with functional genomic screening having an increasingly important
Carlos le Sage +2 more
openaire +3 more sources
Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
Nature Communications, 2023 CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past decade and holds great promise for the treatment of human genetic diseases.
Jianli Tao, D. E. Bauer, R. Chiarle
semanticscholar +1 more source
A Unique B-Family DNA Polymerase Facilitating Error-Prone DNA Damage Tolerance in Crenarchaeota
Frontiers in Microbiology, 2020 Sulfolobus islandicus codes for four DNA polymerases: three are of the B-family (Dpo1, Dpo2, and Dpo3), and one is of the Y-family (Dpo4). Western analysis revealed that among the four polymerases, only Dpo2 exhibited DNA damage-inducible expression.
Xu Feng +7 more
doaj +1 more source