Results 61 to 70 of about 277,742 (368)
CRISPR SWAPnDROP -- A multifunctional system for genome editing and large-scale interspecies gene transfer [PDF]
, 2021 The need for diverse chromosomal modifications in biotechnology, synthetic biology and basic research requires the development of new technologies. With CRISPR SWAPnDROP, we extend the limits of genome editing to large-scale in-vivo DNA transfer between bacterial species.
arxiv +1 more source
CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
Nucleic Acids Res., 2019 The CRISPR–Cas system is a powerful genome editing tool that functions in a diverse array of organisms and cell types. The technology was initially developed to induce targeted mutations in DNA, but CRISPR–Cas has now been adapted to target nucleic acids
Kornel Labun +5 more
semanticscholar +1 more source
CRISPR-C: circularization of genes and chromosome by CRISPR in human cells [PDF]
Nucleic Acids Research, 2018 Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-
Yonglun Luo +17 more
openaire +6 more sources
Today's Biothreats – Where the Past Predictions Meet the Future
Вестник войск РХБ защиты, 2020 The catastrophic spread of COVID-19 pandemic, uncontrolled by modern medical science, regardless of whether it is artificial or not, clearly shows the limits of human mind and knowledge to resist this and other similar challenges.
J. Lakota
doaj +1 more source
Twenty-first Century Dental Practice and the Treatment of Nonmicrobial Genetic-based Dental Diseases
Dental Hypotheses, 2021 After W.D. Miller proved a causal relationship between microbes and dental caries and periodontitis, the repair and replacement of damaged or lost teeth resulting from microbial activity dominated 20th century dental practice.
Edward F Rossomando
doaj +1 more source
Unraveling Mycobacterium tuberculosis acid resistance and pH homeostasis mechanisms
FEBS Letters, EarlyView.Mycobacterium tuberculosis exhibits a remarkable resilience to acid stress. In this Review, we discuss some of the molecular mechanisms and metabolic pathways used by the tubercle bacilli to adapt and resist host‐mediated acid stress. Mycobacterium tuberculosis (Mtb) is a successful pathogen that has developed a variety of strategies to survive and ...
Janïs Laudouze +3 more
wiley +1 more source
Molecular Oncology, EarlyView.Triacsin C inhibition of the acyl‐CoA synthetase long chain (ACSL) family decreases multiple myeloma cell survival, proliferation, mitochondrial respiration, and membrane potential. Made with Biorender.com. Multiple myeloma (MM) is an incurable cancer of plasma cells with a 5‐year survival rate of 59%.
Connor S. Murphy +12 more
wiley +1 more source
Optimal number of spacers in CRISPR arrays [PDF]
PLoS Comput Biol 13(12): e1005891 (2017), 2017 We estimate the number of spacers in a CRISPR array of a bacterium which maximizes its protection against a viral attack. The optimality follows from a competition between two trends: too few distinct spacers make the bacteria vulnerable to an attack by a virus with mutated corresponding protospacers, while an excessive variety of spacers dilutes the ...
arxiv +1 more source
Detection of CRISPR adaptation [PDF]
Biochemical Society Transactions, 2020 Prokaryotic adaptive immunity is built when short DNA fragments called spacers are acquired into CRISPR (clustered regularly interspaced short palindromic repeats) arrays. CRISPR adaptation is a multistep process which comprises selection, generation, and incorporation of prespacers into arrays.
Ivan Fedorov +7 more
openaire +2 more sources
Molecular Oncology, EarlyView.Transient receptor potential melastatin‐4 (TRPM4) is overexpressed in prostate cancer (PCa). Knockout of TRPM4 resulted in reduced PCa tumor spheroid size and decreased PCa tumor spheroid outgrowth. In addition, lack of TRPM4 increased cell death in PCa tumor spheroids.
Florian Bochen +6 more
wiley +1 more source