Results 151 to 160 of about 4,544 (206)
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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1987
Abstract 1. 1. Components of the cell surface of Crithidia guilhermei, Crithidia deanei and Crithidia oncopelti were radioiodinated by the iodogen technique. The distribution of proteins in the detergent-poor (DPP) and detergent-enriched phase (DRP) were studied using a phase separation technique in Triton X-114 and one- and two-dimensional ...
S.Giovanni De Simone +3 more
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Abstract 1. 1. Components of the cell surface of Crithidia guilhermei, Crithidia deanei and Crithidia oncopelti were radioiodinated by the iodogen technique. The distribution of proteins in the detergent-poor (DPP) and detergent-enriched phase (DRP) were studied using a phase separation technique in Triton X-114 and one- and two-dimensional ...
S.Giovanni De Simone +3 more
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Nucleoside transport in Crithidia luciliae
International Journal for Parasitology, 1993Nucleoside transport was evaluated in the trypanosomatid Crithidia luciliae by a rapid sampling technique. C. luciliae was shown to possess two independent nucleoside transporters, one which transported adenosine, deoxyadenosine, tubercidin, sangivamycin and the pyrimidine nucleoside thymidine, while the second was specific for guanosine, inosine and ...
S T, Hall, G A, Odgers, A M, Gero
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Crithidia factors in human urine
Biochemical Medicine, 1974Abstract 7,8-Dihydrobiopterin is the major component of urinary activity for Crithidia fasciculata together with trace amounts of probably 5,6,7,8-tetrahydrobiopterin. Epileptics controlled by anticonvulsants and patients with rheumatoid arthritis have significantly lower levels of urinary Crithidia factor.
R J, Leeming, J A, Blair
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Auxotrophic Mutants of Crithidia fasciculata
The Journal of Parasitology, 1990In pursuance of genetic studies, after exposure to ethylmethanesulfonate, 11 auxotrophic mutants of Crithidia fasciculata were cloned. Three proved uracil dependent; 3 serine dependent; and the remainder have not had their auxotrophy defined.
L A, Garcia, I, Roitman
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Glucose transport in Crithidia luciliae
Molecular and Biochemical Parasitology, 1992The glucose analogue, 2-deoxy-D-glucose, was used to characterise the glucose transport system in Crithidia luciliae choanomastigotes. Uptake was temperature dependent with a Q10 of 2, and saturable with a Km of 0.22 mM and Vmax of 5.5 nmol min-1 (mg protein)-1 at 23 degrees C.
L A, Knodler +2 more
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Crithidia fasciculata: Characterization of polysaccharide
Experimental Parasitology, 1972Abstract A carbohydrate contaminant was discovered in phenol extracts of the insect hemoflagellate, Crithidia fasciculata . Ion-exchange chromatography was used to separate the polysaccharide from the cellular RNA. Chromatographic, electrophoretic, and colorimetric analyses of the hydrolyzed polysaccharide indicate that it is composed of mannose.
M, Gottlieb, P A, Lanzetta, J, Berech
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Serum Crithidia levels in disease
Biochemical Medicine, 1980Abstract Previous papers recorded levels of crithidia active compounds in sera of normal and diseased subject (1,2). Elucidation of the primary metabolic defect in malignant hyperphenyl-alaniaemia as greatly reduced cell levels of tetrahydrobiopterin, arising from either inadequate biosynthesis or its diminished regeneration from quinonoid ...
R J, Leeming, J A, Blair
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Oxidative phosphorylation in Crithidia fasciculata
Biochemical and Biophysical Research Communications, 1967Abstract Respiratory chain-linked phosphorylation has been demonstrated in cell-free extracts from the protozoan Crithidia fasciculata . The preparations were capable of coupling phosphate esterification to respiration, but were not “tightly coupled”.
J J, Toner, M M, Weber
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The Purine Phosphoribosyltransferases of Crithidia fasciculata
The Journal of Parasitology, 1979The purine phosphoribosyltransferases of Crithidia fasciculata were identified and some of their properties described. The organism possesses three separate enzymes for the production of AMP, IMP, and GMP. The evidence for this comes from the observed differences in elution patterns from gel filtration columns, differences in heat sensitivity, and ...
G W, Kidder, L L, Nolan, V C, Dewey
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Biosynthesis of phosphatidylinositol in Crithidia fasciculata
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980Microsomal preparations from the protozoan (Crithidia fasciculata were shown to incorporate myo-[2-3H]inositol into phosphatidylinositol by both the CDPdiacylglycerol:myo-inositol phosphatidyltransferase reaction and by a myo-inositol exchange reaction. Non-ionic detergent and Mg2+ were necessary for the measurement of transferase activity.
C J, Daniels, F B, Palmer
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