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Glucosylation of glycoproteins in Crithidia fasciculata

Molecular and Biochemical Parasitology, 1991
High mannose-type, N-linked oligosaccharides devoid of glucose units may be glucosylated directly from UDP-Glc in mammalian, plant, fungal and protozoan cells. The glucosylated compounds thus formed (protein-linked Glc1Man5-9GlcNAc2, depending on the organisms) are immediately deglucosylated by glucosidase II, an enzyme located, the same as the ...
G, Gotz, S, Gañán, A J, Parodi
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Aerobic metabolism of Crithidia fasciculata

Experimental Parasitology, 1960
1. 1. Asparagine, aspartate, glucose, glutamate, glutamine, alanine, isocitrate, and oxalacetate (at pH 7.4) stimulated the oxygen consumption of intact cells of C. fasciculata. 2. 2. Krebs' tricarboxylic acid cycle intermediates (excepting isocitrate and oxalacetate) did not stimulate the oxygen consumption of the intact cells at the same pH.
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Culture and Genetic Modification of the Monoxenous Trypanosomatids Crithidia fasciculata and Crithidia bombi

Monoxenous trypanosomatids exclusively parasitize insects and are powerful systems for exploring trypanosomatid biology, the evolution of parasitism, and host-parasite interactions. In addition, they are tractable early-diverging eukaryotes that can serve as model organisms for a variety of conserved processes.
Blyssalyn V, Bieber   +5 more
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Pathogenicity of "Crithidia fasciculata" in the haemocoele of "Glossina"

Acta tropica, 1987
The mosquito flagellate Crithidia fasciculata produces intense haemocoelic infections following intrahaemocoelic inoculation into 5 species of Glossina--G. austeni, G. fuscipes fuscipes, G. morsitans morsitans, G. palpalis gambiensis and G. tachinoides. All Glossina inoculated with C. fasciculata died between days 4 and 9.
E A, Ibrahim, D H, Molyneux
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The sterols of Trypanosoma cruzi and Crithidia fasciculata

Comparative Biochemistry and Physiology, 1969
Abstract 1. 1. Six sterols have been detected in Trypanosoma cruzi cultured on serum-free medium. Four of these are ergosterol, 22,23-dihydroergosterol, 7-dehydroporiferasterol and 7-dehydroclionasterol (or their C24 isomers from which they cannot be distinguished with the small quantities available).
E D, Korn, T, Von Brand, E J, Tobie
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Effects of Hydroxyurea on Crithidia fasciculata

The Journal of Protozoology, 1979
SYNOPSIS Hydroxyurea (HU) inhibits increase in cell number in cultures of Crithidia fasciculata. Complete inhibition is produced by 8 mM and higher concentrations. If HU is not removed, population growth resumes in 45–50 h: if HU is removed, partially synchronous growth occurs through 2 cycles. During HU inhibition, the rate of DNA synthesis is reduced
W B, Cosgrove, M J, Skeen, S L, Hajduk
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The histones of the insect trypanosomatid, Crithidia fasciculata

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1990
The histone-like proteins of the monogenetic parasite Crithidia fasciculata were extracted with 0.2 M sulfuric acid either from purified nuclei, or from purified chromatin, in both cases in the presence of 1 mM tosyl lysylchloromethylketone and 2 mM phenyl methyl sulfonyl fluoride as proteinase inhibitors.
V G, Duschak, J J, Cazzulo
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Crithidia fasciculata: A Test For Genetic Exchange

Experimental Parasitology, 2001
Abstract Votýpka, J., Ray D. S., and Lukes J. 2001. Crithidia fasciculata: A test for genetic exchange. Experimental Parasitology99, 104–107.
J, Votýpka, D S, Ray, J, Lukes
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The origin of unconjugated pteridines in Crithidia fasciculata

Archiv f�r Mikrobiologie, 1967
By using folic acid-2-14C in the growth medium as the sole source of pteridine it was found that the kinetoplastid flagellate, Crithidia fasciculata, produced a total of 87 mμg of labeled biopterin and 23 μg of 2-amino-4-hydroxy-6-hydroxymethylpteridine from 2 mg of folic acid in 2 liters of medium.
G W, Kidder, V C, Dewey, H, Rembold
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Arylamidases of Crithidia fasciculata

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1974
Abstract 1. 1. Crithidia fasciulata was found to be arich source of soluble enzymes hydrolysing the β-naphthylamides of leucine, arginine, lysine and glutamic acid. The specific activities were similar to values observed in mammalian tissues except for higher arylamidase A (α-glu) activity. 2. 2. Arylamidase A (α-glu) was stimulated twofold
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