Results 61 to 70 of about 11,237,275 (282)

The potential of microfluidic cell analysis in CAR T cell therapy manufacturing

Frontiers in Bioengineering and Biotechnology
Despite significant success in treating hematological cancers, Chimeric Antigen Receptor (CAR) T cell therapies must overcome several challenges to become accessible to a wide patient population.
Aleksandra Nikoniuk, Aleksandra Nikoniuk, Koki Lilova, Michael Thomas, Michael Thomas, Nicolas Szita, Nicolas Szita   +6 more
doaj   +1 more source

Influence of Incident Wavelength and Detector Material Selection on Fluorescence in the Application of Raman Spectroscopy to a Fungal Fermentation Process

Bioengineering, 2018
Raman spectroscopy is a novel tool used in the on-line monitoring and control of bioprocesses, offering both quantitative and qualitative determination of key process variables through spectroscopic analysis. However, the wide-spread application of Raman
Stephen Goldrick, David Lovett, Gary Montague, Barry Lennox   +3 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Applying Raman Spectroscopy for Real-Time Monitoring of Ultra- and Diafiltration Steps in Viral Vector Purification

Pharmaceutics
Background: Process analytical technology (PAT) enhances product quality by monitoring and controlling critical quality attributes (CQAs), offering better insights into process performance.
Cláudia S. Paiva, Hadi El Radi, Diogo Chagas, Kévin Grollier, Johan Cailletaud, Sébastien Delacroix, Paolo Gabaldi, Tiago Q. Faria, Cristina Peixoto   +8 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Quality by design driven development of a Monteverdia ilicifolia herbal medicine: from plant to granules

Frontiers in Natural Products
IntroductionMonteverdia ilicifolia (Mart. Ex Reiss.) Biral, popularly known as espinheira-santa, is a medicinal plant recommended in Brazilian primary healthcare due to its well-recognized antidyspeptic, antacid, and gastric protective properties ...
Alberto Araujo de Caux, Alberto Araujo de Caux, José Cláudio Klier Monteiro Filho, Ruan Barbosa da Silva e Medeiros, Marili Villa Nova Rodrigues, Marcos Nopper Alves, Marcus Bruno Soares Forte, Maria Isabel Rodrigues, Maria Isabel Rodrigues, Rodney Alexandre Ferreira Rodrigues, Rodney Alexandre Ferreira Rodrigues   +10 more
doaj   +1 more source

Quality by Design: Development of the Quality Target Product Profile (QTPP) for Semisolid Topical Products

Pharmaceutics, 2020
In recent years, the “quality by design” (QbD) approach has been used for developing pharmaceutical formulations. This is particularly important for complex dosage forms such as topical semisolid products.
Sarika Namjoshi, Maryam Dabbaghi, Michael S. Roberts, Jeffrey E. Grice, Yousuf Mohammed   +4 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source