PhoX – an IMAC-enrichable Crosslinking Reagent [PDF]
ACS Central Science, 2019 AbstractChemical crosslinking mass spectrometry is rapidly emerging as a prominent technique to study protein structures. Structural information is obtained by covalently connecting peptides in close proximity by small reagents and identifying the resulting peptide pairs by mass spectrometry.
Steigenberger, Barbara A. +3 more
openaire +7 more sources
Application of proanthocyanidin in the treatment and repair of deep caries
口腔疾病防治, 2023 Proanthocyanidin (PA), as a kind of natural plant polyphenol, have a variety of biological functions, such as promoting remineralization, inducing collagen cross-linking, inhibiting protease activity and inhibiting bacteria.
GAO Ying +7 more
doaj +1 more source
ECL 3.0: a sensitive peptide identification tool for cross-linking mass spectrometry data analysis
BMC Bioinformatics, 2023 Background Cross-linking mass spectrometry (XL-MS) is a powerful technique for detecting protein–protein interactions (PPIs) and modeling protein structures in a high-throughput manner.
Chen Zhou +6 more
doaj +1 more source
Molecular dynamics simulations with grand-canonical reweighting suggest cooperativity effects in RNA structure probing experiments [PDF]
J. Chem. Theory Comput, 19, 3672 (2023), 2022 Chemical probing experiments such as SHAPE are routinely used to probe RNA molecules. In this work, we use atomistic molecular dynamics simulations to test the hypothesis that binding of RNA with SHAPE reagents is affected by cooperative effects leading to an observed reactivity that is dependent on the reagent concentration.
arxiv +1 more source
Structure of the ovarian lactogen receptors. Analysis with bifunctional cross-linking reagents.
Journal of Biological Chemistry, 1984 J. Bonifacino, M. Dufau
semanticscholar +2 more sources
Cleavage and cross-linking of proteins with osmium (VII) reagents. [PDF]
Journal of Histochemistry & Cytochemistry, 1982 The specific cleavage of proteins following reaction with osmium tetroxide is slowed down by tertiary amines which are known to be good ligands for osmium. Bidentate ligands are more effective than monodentate ligands. These same ligands also promote an intermolecular cross-linking reaction.
E. J. Behrman, Michael Emerman
openaire +3 more sources
Platelet protein organization: analysis by treatment with membrane-permeable cross-linking reagents.
Blood, 1982 We have examined platelet protein organization by treatment of intact resting or thrombin-activated platelets with two cross-linking reagents, diamide or dithiobis(succinimidyl propionate) (DTSP).
G. E. Davies, J. Palek
semanticscholar +1 more source
Mass Spectrometry Evidence for Cisplatin As a Protein Cross-Linking Reagent [PDF]
Analytical Chemistry, 2011 Cisplatin is a potent anticancer drug, which functions by cross-linking adjacent DNA guanine residues. However within 1 day of injection, 65-98% of the platinum in the blood plasma is protein-bound. It is generally accepted that cisplatin binds to methionine and histidine residues, but what is often underappreciated is that platinum from cisplatin has ...
Huilin Li +6 more
openaire +4 more sources
The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents [PDF]
, 1990 Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 34-kD integral membrane glycoprotein (Wiedmann, M., T. V. Kurzchalia, E. Hartmarm, and T. A. Rapoport. 1987. Nature
Dobberstein, Bernhard +9 more
core +1 more source
Enzyme-catalysed polymer cross-linking:Biocatalytic tools for chemical biology, materials science and beyond [PDF]
, 2020 Intermolecular cross-linking is one of the most important techniques that can be used to fundamentally alter the material properties of a polymer. The introduction of covalent bonds between individual polymer chains creates 3D macromolecular assemblies ...
Maddock, Rosie M A +4 more
core +3 more sources