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Improvement of the CRISPR-Cpf1 system with ribozyme-processed crRNA [PDF]
RNA Biology, 2018 The recently discovered clustered regularly interspaced short palindromic repeats (CRISPR)-Cpf1 system expands the genome editing toolbox. This system exhibits several distinct features compared to the widely used CRISPR-Cas9 system, but has reduced gene editing efficiency.
Elena Herrera-Carrillo +2 more
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A Single Multiplex crRNA Array for FnCpf1-Mediated Human Genome Editing [PDF]
Molecular Therapy, 2018 Cpf1 has been harnessed as a tool for genome manipulation in various species because of its simplicity and high efficiency. Our recent study demonstrated that FnCpf1 could be utilized for human genome editing with notable advantages for target sequence selection due to the flexibility of the protospacer adjacent motif (PAM) sequence.
Jia Qu, Zongming Song, Huihui Sun
exaly +3 more sources
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Computational Detection of CRISPR/crRNA Targets
2015The CRISPR-Cas systems in bacteria and archaea provide protection by targeting foreign nucleic acids. The sequence of the "spacers" within CRISPR arrays specifically determines the targets in invader genomes. These spacers provide the short specific RNA nucleotide sequences within the guide crRNAs.
Ambarish, Biswas +2 more
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Analysis of CRISPR Pre-crRNA Cleavage
2015We have examined the processing of precursor-clustered regularly interspaced short palindromic repeat (CRISPR) RNAs (pre-crRNAs) of the Type I CRISPR-Cas system by incubation of radiolabeled model RNAs with recombinant CRISPR-associated (Cas) endoribonucleases, followed by denaturing polyacrylamide gel electrophoresis (PAGE) of the products ...
Erin L, Garside, Andrew M, MacMillan
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Array Assembler Provides Greatly Simplified crRNA Array Design for CRISPR Cas12 and Cas13 Variants
ACS Synthetic BiologyAs newer CRISPR variants have emerged and corresponding toolkits have been developed, researchers can now readily target multiple genes simultaneously for knockout, activation, or repression alongside being able to bind or cleave mRNA. However, as larger
James W Bryson
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Engineered crRNA for CRISPR/Cas-assisted biosensing
Trends in BiotechnologyCRISPR/Cas-based diagnostics (CRISPR-Dx) face challenges, including difficulty in detecting ultrashort nucleotides, preamplification dependency, cross-contamination, insufficiency in on-pot detection paradigms, and inconvenience in detecting non-nucleic acid targets.
Long Ma +6 more
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cliCRISPR: crRNA-limited CRISPR/Cas12a system for multiplexed detection
Biosensors and BioelectronicsThe CRISPR/Cas12a system is extensively employed for nucleic acid detection, where crRNA is typically administered in excess to quantify target levels. However, its non-specific trans-cleavage activity poses challenges for achieving multiplexed detection within a single-pot CRISPR/Cas12a assay.
Bo Yan +4 more
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Harnessing crRNA Transformer for Facile and Specific Nucleic Acid Detection
Analytical ChemistryCRISPR/Cas systems have emerged as promising tools for nucleic acid detection. However, their practical applications have been limited by poor specificity and the need for additional preprocessing steps. Inspired by the concept of transformers, we found that changing the forms of crRNA with spatial arrangement may endow CRISPR/Cas with an enhanced ...
Mei Wen +14 more
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Split crRNAs Enhance Cas12a Diagnostic Performance
ChemBioChemCRISPR RNA (crRNA) is the key guide molecule in the CRISPR–Cas12a system, directing the Cas protein to recognize target sequences. It consists of a repeat‐derived stem loop that binds Cas12a and helps stabilize the ribonucleoprotein complex, and a spacer region that base‐pairs with the target and determines recognition specificity.
Xinyi Guo, Tian Tian, Xiaoming Zhou
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