Results 201 to 210 of about 14,674 (226)
Some of the next articles are maybe not open access.
CRISPR–Cas12a with split crRNA for the direct and sensitive detection of microRNA
The AnalystA split crRNA-activated Cas12a-based method enables amplification-free miRNA-21 detection. This method demonstrates enhanced sensitivity and specificity, achieving the accurate quantification of miRNA-21 in serum.
Sijia Yang +7 more
openaire +2 more sources
RNA-mediated CRISPR-Cas13 inhibition through crRNA structural mimicry
ScienceTo circumvent CRISPR-Cas immunity, phages express anti-CRISPR factors that inhibit the expression or activities of Cas proteins. Whereas most anti-CRISPRs described to date are proteins, recently described small RNAs called RNA anti-CRISPRs (rAcrs) have sequence homology to CRISPR RNAs (crRNAs) and displace them from cognate Cas nucleases.
Victoria M. Hayes +7 more
openaire +2 more sources
Nitroreductase-Responsive Oligomeric crRNAs for Enzyme-Triggered Regulation of CRISPR Activity
The Journal of Organic ChemistryHypoxic tumors overexpress nitroreductase (NTR), providing an endogenous trigger for selective biomolecular activation. Here, we describe the synthesis of NTR-responsive clustered regularly interspaced short palindromic repeats (CRISPR) guide RNAs via the site-specific incorporation of a p-nitrobenzyl (p-NB) phosphoramidite at the 5' terminus of crRNAs.
Wen-Da Chen, Li Liu, Liang Cheng
openaire +2 more sources
Allosteric ribozyme-driven crRNA switch for the amplification-free detection of biomolecules
Biosensors and BioelectronicsCurrently, CRISPR-mediated biosensors are concentrating on the design of the crRNA or the activator strand to regulate the trans-cleavage activity of Cas12a. Herein, we report an allosteric ribozyme-driven crRNA switch-regulated CRISPR/Cas12a sensor for amplification-free detection of biomolecules.
Mei Su +4 more
openaire +2 more sources
Cryo-EM structure of the RfxCas13d-crRNA-off-target-RNA complex
StructureThe CRISPR-Cas system is crucial for the adaptive immune response of prokaryotes and has been widely applied for genetic engineering. Cas13d, a type VI-D CRISPR-Cas effector, functions as RNA-guided ribonuclease and has been engineered for programmable RNA editing, which is a commonly used, active, and well-characterized small type VI editor.
Qianxi Yang +4 more
openaire +2 more sources
Rapid and sensitive Cas12a-based one-step nucleic acid detection with ssDNA-modified crRNA
Analytica Chimica Acta, 2023Zhuo Li, Qinlong Zeng, Zhiqing Hu
exaly
Optical Control of a CRISPR/Cas9 System for Gene Editing by Using Photolabile crRNA
Angewandte Chemie - International Edition, 2020Tao Liu, Xinyu Ling, Xinjing Tang
exaly
Improving the efficiency of the CRISPR-Cas12a system with tRNA-crRNA arrays
Crop Journal, 2020Xiangbing Meng, Li Jiayang, Hong Yu
exaly