Results 211 to 220 of about 6,328,527 (298)

Cryo-EM structures of apo, agonist- and antagonist-bound heteromeric kainate receptors. [PDF]

Sci Adv
Zhou C, Segura-Covarrubias G, M'Kay Hoffman A, Tajima N.   +3 more
europepmc   +1 more source

DNA Nanotechnology Meets Peptide and Protein Self‐Assembly

Chemistry – A European Journal, EarlyView.
Combining DNA nanotechnology with peptide and protein assembly provides complementary platforms for the rational engineering of functional biomaterials. This Perspective discusses the emerging field of self‐assembling DNA‐peptide and DNA‐protein hybrid systems that combine the structural precision and programmability of DNA nanotechnology with the ...
Marcel Hanke, Nanjundan Raghul, Adrian Keller, Veikko Linko, Verónica I. Dodero   +4 more
wiley   +1 more source

Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor. [PDF]

IUCrJ
Crawford J, Munuganti R, Leung C, Singh K, Gates E, Zhu X, Bally M, Santos ND, Sharifiaghdam M, Nosrati Z, Axerio-Cilies P, Berezuk AM, Cholak S, Merk A, Cameron DR, Subramaniam S.   +15 more
europepmc   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Affinity purification contaminants identified by cryo-EM and mass spectrometry. [PDF]

Biosci Rep
Belcher ER, Hardwick SW, Maia de Oliveira T, Hyvönen M.   +3 more
europepmc   +1 more source

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

Cytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham, Mihir Relan, Matthew T. Swulius, Andrea K. H. Stavoe   +3 more
wiley   +1 more source

CryoWriter: a robotic solution for improved Cryo-EM grid preparation. [PDF]

Nat Commun
Kv C, Ekundayo B, Di Fabrizio M, Mohammed I, Radecke J, Stahlberg H, Kube M.   +6 more
europepmc   +1 more source

Microtubule Destabilizing Kinesins: A Historical Perspective and Thoughts for the Future

Cytoskeleton, EarlyView.
ABSTRACT The microtubule cytoskeleton plays critical roles in multiple cellular processes such as mitotic spindle assembly and chromosome segregation. Cells contain a number of microtubule‐associated proteins that regulate microtubule dynamics both temporally and spatially in cells. The microtubule depolymerizing kinesins include members of the Kinesin‐
Claire E. Walczak
wiley   +1 more source

Cryo-EM structures of biopsy-derived TTR fibrils in hereditary transthyretin amyloidosis. [PDF]

Nat Commun
Zheng Y, Liang J, Li Z, Liu Y, Chu X, Zhang S, Wang W, Bao J, Liu B, Ma C, Yin G, Deng J, Chi W, Meng L, Shi Y.   +14 more
europepmc   +1 more source

Assembly and Maintenance of Myofibrils in the Heart

Cytoskeleton, EarlyView.
ABSTRACT The assembly of actin and myosin to the paracrystalline arrangement of myofibrils in striated muscle, that is, heart and skeletal, has puzzled many a cell biologist. Different models, based on cultured cardiomyocytes, were proposed for the last 30 years to explain the sequence of events.
Elisabeth Ehler
wiley   +1 more source