Results 231 to 240 of about 6,313,615 (343)

Digital twin of biomacromolecular thermodynamics in cryo-EM data. [PDF]

Commun Chem
Yamazaki K, Wada M, Wada Y, Tokuhisa A, Ohga K, Nakagawa A, Akinaga Y, Sasakura Y, Katoh T, Matsumoto S, Okuno Y.   +10 more
europepmc   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Confidence-guided cryo-EM map optimisation with LocScale-2.0. [PDF]

Nat Commun
Bharadwaj A, de Bruin R, Jakobi AJ.
europepmc   +1 more source

MICA 2026 cryo-EM benchmark: dataset and benchmark outputs

Aravinda Venkatesan
openalex   +1 more source

Cryo-EM Reveals VP1/VP2 N-Terminal Conformations in Adeno-Associated Virus 2 Capsids [PDF]

Innocent O. Osuya, S. Shirin Kamalaldinezabadi, Ananya Sengupta, Gwimoon Seo, Michael Stewart Chapman, Scott M. Stagg   +5 more
openalex   +1 more source

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

Cytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham, Mihir Relan, Matthew T. Swulius, Andrea K. H. Stavoe   +3 more
wiley   +1 more source

Validation reports, models and cryo-EM maps

Patrick Scheerer
openalex   +1 more source

Microtubule Destabilizing Kinesins: A Historical Perspective and Thoughts for the Future

Cytoskeleton, EarlyView.
ABSTRACT The microtubule cytoskeleton plays critical roles in multiple cellular processes such as mitotic spindle assembly and chromosome segregation. Cells contain a number of microtubule‐associated proteins that regulate microtubule dynamics both temporally and spatially in cells. The microtubule depolymerizing kinesins include members of the Kinesin‐
Claire E. Walczak
wiley   +1 more source

Structural physiology of distinctive water channels elucidated by cryo-EM. [PDF]

IUCrJ
Kozai D, Suzuki S, Kamegawa A, Fujiyoshi Y.   +3 more
europepmc   +1 more source

Assembly and Maintenance of Myofibrils in the Heart

Cytoskeleton, EarlyView.
ABSTRACT The assembly of actin and myosin to the paracrystalline arrangement of myofibrils in striated muscle, that is, heart and skeletal, has puzzled many a cell biologist. Different models, based on cultured cardiomyocytes, were proposed for the last 30 years to explain the sequence of events.
Elisabeth Ehler
wiley   +1 more source