Results 111 to 120 of about 108,976 (266)
Biotechnology and Bioengineering, EarlyView.ABSTRACT Stem cell‐based therapies hold transformative potential for regenerative medicine, yet their progress is constrained by persistent variability in manufacturing and clinical performance. Conventional frameworks such as Quality by Design (QbD), though effective for drugs and biologics, struggle to accommodate the dynamic nature of living cell ...
Mee‐Hae Kim, Masahiro Kino‐oka
wiley +1 more source
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach
Biotechnology Progress, EarlyView.Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya +7 more
wiley +1 more source
Possibilities and limitations of vegetative propagation in breeding and mass propagation of Norway spruce [PDF]
, 2003 The use of vegetative mass propagation in practical forestry with Norway spruce (Picea abies (L.) Karst.) is limited at present, although its potential to deliver high genetic gains is obvious.
Högberg, Karl-Anders
core
Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning
Cytoskeleton, EarlyView.ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham +3 more
wiley +1 more source
Systematic alteration of ATAC-seq for profiling open chromatin in cryopreserved nuclei preparations from livestock tissues. [PDF]
, 2020 The use of Assay for Transposase-Accessible Chromatin (ATAC-seq) to profile chromatin accessibility has surged over the past years, but its applicability to tissues has been very limited.
Chanthavixay, G +7 more
core +1 more source
Micro Pattern‐Based 3D Cell Culture Platform: An Overview of Technologies and Applications
Exploration, EarlyView.Micro patterns are characterized as a coating of specific adhesion matrices on substrates to support cell growth. An overview of micropattern technology to regulate single cell morphology and fates, further focusing on the micro pattern‐based 3D cell culture platform used in the non‐tumor and tumor researches.
Xinglong Zhu +8 more
wiley +1 more source
Exploration, EarlyView.ADF is prepared as a cell‐free bio‐membrane from autologous adipose ECM using simple physical techniques inspired by “papermaking”. It boasts superior properties and facilitates tissue regeneration through Trem2+ macrophages‐mediated angiogenesis. ADF can be stored long‐term for personalized medicine, offering insights into autologous ECM preservation ...
Mengmeng Hou +8 more
wiley +1 more source
BMJ OpenObjectives The present study was designed to examine the attitudes towards oocyte cryopreservation among healthcare providers working in hospitals across specialties and potential influencing factors.Design A cross-sectional study.Setting The ...
Jingjing Lu, Xuezi Tian, Zhaochen Wang
doaj +1 more source
Five-dimensional imaging of freezing emulsions with solute effects
, 2018 The interaction of objects with a moving solidification front is a common feature of many industrial and natural processes such as metal processing, the growth of single-crystals, the cryopreservation of cells, or the formation of sea ice. Solidification
Dedovets, Dmytro +2 more
core +3 more sources