Results 41 to 50 of about 166,403 (367)

A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. [PDF]

PLoS ONE, 2014
Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). Vitrification requires considerable skill and post-thaw recovery is low.
Keitaro Imaizumi, Naoki Nishishita, Marie Muramatsu, Takako Yamamoto, Chiemi Takenaka, Shin Kawamata, Kenichiro Kobayashi, Shin-Ichi Nishikawa, Teruo Akuta   +8 more
doaj   +1 more source

Effect of Cryopreservation on the Ex Vitro Establishment of Olive Plants Regenerated via Somatic Embryogenesis

Plants, 2021
Cryopreservation is considered the best technique for the safe, long-term conservation of embryogenic cultures. However, before integrating it into a somatic embryogenesis system, the influence of cryopreservation on the final production of plants should
Fatiha Bradaï, Carolina Sánchez-Romero
doaj   +1 more source

Stepped vitrification technique for human ovarian tissue cryopreservation [PDF]

, 2019
The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation.
Amorin, C.A., Camboni, A., Corral, A., Dolmans, M.M., Kilbride, P., Leonel, E.C.R., Morris, John G., Risco, Ramón, Taboga, S.R., Vázquez, Marina   +9 more
core   +1 more source

Freeze/thaw stress induces organelle remodeling and membrane recycling in cryopreserved human mature oocytes [PDF]

, 2016
Purpose: Our aim was to evaluate the ultrastructure of human metaphase II oocytes subjected to slow freezing and fixed after thawing at different intervals during post-thaw rehydration.
Albani, E., Borini, A., Coticchio, G., Levi Setti, P. E., Lorenzo, C., Macchiarelli, G., NOTTOLA, Stefania Annarita, Palmerini, M. G., Scaravelli, G.   +8 more
core   +1 more source

Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges

Animals, 2022
Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro, David F. Carrageta, Raquel L. Bernardino, Marco G. Alves, Pedro F. Oliveira   +4 more
doaj   +1 more source

Cryopreservation of equine oocytes: looking into the crystal ball [PDF]

, 2020
In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application.
Angel Velez, Daniel, De Coster, Tine, Smits, Katrien, Van Soom, Ann, Woelders, Henri   +4 more
core   +2 more sources

Role of Viral Infections in Testicular Cancer Etiology: Evidence From a Systematic Review and Meta-Analysis

Frontiers in Endocrinology, 2019
The most represented histotype of testicular cancer is the testicular germ-cell tumor (TGCT), both seminoma and non-seminoma. The pathogenesis of this cancer is poorly known.
Andrea Garolla, Amerigo Vitagliano, Francesco Muscianisi, Umberto Valente, Marco Ghezzi, Alessandra Andrisani, Guido Ambrosini, Carlo Foresta   +7 more
doaj   +1 more source

Perfusion, cryopreservation, and nanowarming of whole hearts using colloidally stable magnetic cryopreservation agent solutions

Science Advances, 2021
Colloidally stable nanoparticles and magnetic cryopreservation agents can be used for cryopreservation and nanowarming of hearts. Nanowarming of cryopreserved organs perfused with magnetic cryopreservation agents (mCPAs) could increase donor organ ...
Andreina Chiu-Lam, Edward M. Staples, C. Pepine, C. Rinaldi   +3 more
semanticscholar   +1 more source

Flow Cytometry of the Side Population: Tips & Tricks

Cellular Oncology, 2006
Background: The Side Population (SP) has become an important hallmark for the definition of the stem cell compartment, especially in the detection of these cells and in their physical isolation by fluorescence-activated cell sorting (FACS).
Irene Sales-Pardo, Ariadna Avendaño, Vanessa Martinez-Muñoz, Marta García-Escarp, Raquel Celis, Phil Whittle, Jordi Barquinero, Joan Carles Domingo, Pedro Marin, Jordi Petriz   +9 more
doaj   +1 more source

Vitrification of human immature oocytes before and after in vitro maturation: a review [PDF]

, 2017
The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation
Ashourzadeh, S, Khalili, Ma, Macchiarelli, G, Nottola, Sa, Palmerini, Mg, Shahedi, A   +5 more
core   +1 more source