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Plants, 2021 Cryopreservation is considered the best technique for the safe, long-term conservation of embryogenic cultures. However, before integrating it into a somatic embryogenesis system, the influence of cryopreservation on the final production of plants should
Fatiha Bradaï, Carolina Sánchez-Romero
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Stepped vitrification technique for human ovarian tissue cryopreservation [PDF]
, 2019 The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation.
Amorin, C.A. +9 more
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Cryopreservation: A Review Article
Cureus, 2022 Organelles, cells, tissues, or any other biological construction can be preserved using a method called cryopreservation, in which samples are cooled to extremely low temperatures.
Anurag N Jaiswal, Anjali A Vagga
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Freeze/thaw stress induces organelle remodeling and membrane recycling in cryopreserved human mature oocytes [PDF]
, 2016 Purpose: Our aim was to evaluate the ultrastructure of human metaphase II oocytes subjected to slow freezing and fixed after thawing at different intervals during post-thaw rehydration.
Albani, E. +8 more
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Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges
Animals, 2022 Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro +4 more
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Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing [PDF]
, 2015 Byrsonima intermedia is a shrub from the Brazilian Cerrado with medicinal properties. The storage of biological material at ultra-low temperatures (-196°C) is termed cryopreservation and represents a promising technique for preserving plant diversity ...
Andre, E. +4 more
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Frontiers in Endocrinology, 2019 The most represented histotype of testicular cancer is the testicular germ-cell tumor (TGCT), both seminoma and non-seminoma. The pathogenesis of this cancer is poorly known.
Andrea Garolla +7 more
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Cryoprotectant-free vitrification of human spermatozoa in new artificial seminal fluid [PDF]
, 2016 Vitrification is a new method that has been recently introduced in Assisted Reproduction Technique programs. The aim of this study was to design a new medium similar to normal human seminal fluid (SF), formulation artificial seminal fluid (ASF), and to ...
Agha Rahimi, A. +4 more
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Step by step optimization of a sperm cryopreservation protocol for spotted wolffish (Anarhichas minor Olafsen, 1772) [PDF]
, 2020 Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, low sperm volume with relatively low cell concentration and the lack of gametes synchronization (simultaneous availability of mature eggs and ...
Beirão, J. +3 more
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Flow Cytometry of the Side Population: Tips & Tricks
Cellular Oncology, 2006 Background: The Side Population (SP) has become an important hallmark for the definition of the stem cell compartment, especially in the detection of these cells and in their physical isolation by fluorescence-activated cell sorting (FACS).
Irene Sales-Pardo +9 more
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