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Cryopreservation by Directional Freezing and Vitrification Focusing on Large Tissues and Organs
The cryopreservation of cells has been in routine use for decades. However, despite the extensive research in the field, cryopreservation of large tissues and organs is still experimental.
A. Arav
semanticscholar +1 more source
Antifreeze proteins (AFPs), a class of antifreeze substances, were initially found to bind to ice crystals in vivo, thereby contributing to inhibition of the growth of ice crystals that enabled polar fish to survive at the temperatures below freezing ...
HENG Nuo+8 more
doaj +1 more source
A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. [PDF]
Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). Vitrification requires considerable skill and post-thaw recovery is low.
Keitaro Imaizumi+8 more
doaj +1 more source
Colloidally stable nanoparticles and magnetic cryopreservation agents can be used for cryopreservation and nanowarming of hearts. Nanowarming of cryopreserved organs perfused with magnetic cryopreservation agents (mCPAs) could increase donor organ ...
Andreina Chiu-Lam+3 more
semanticscholar +1 more source
An efficient, economical slow-freezing method for large-scale human embryonic stem cell banking [PDF]
Human embryonic stem cells (hESCs) are one of the most interesting cell types for tissue engineering, cell therapy, basic scientific research, and drug screening. Fast advancement in these areas requires the availability of large amounts of safe and well-
Cornelissen, Maria+3 more
core +2 more sources
Cryopreservation of equine oocytes: looking into the crystal ball [PDF]
In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application.
Angel Velez, Daniel+4 more
core +2 more sources
ROS-induced oxidative stress in plant cryopreservation: occurrence and alleviation
Reactive oxygen species (ROS)-induced oxidative stress results in low success or even total failure of cryopreservation. Better understanding of how the plant establishes resistance/tolerance to ROS-induced oxidative stress facilitates developments of ...
Li Ren, Min‐Rui Wang, Qiaochun Wang
semanticscholar +1 more source
Cryopreservation has been successfully used in the banking and maintenance of cultures of microorganisms, from bacteria to yeasts, since the onset of cryobiology. Biobanking of marine biological resources is crucial for development of scientific knowledge as researchers rely on guaranteed access to reliable, stable resources. Culture collections play a
Paredes Rosendo, Estefanía+4 more
openaire +4 more sources
Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges
Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro+4 more
doaj +1 more source
Cryopreservation of common carp sperm [PDF]
Experiments were carried out to investigate the effect of five extenders (sucrose, glucose, fructose, KCl and a saline carp sperm extender) and two cryoprotectants (dimethyl-sulfoxide (DMSO) and methanol) on the cryopreservation of common carp sperm ...
Horváth, Ákos+2 more
core +1 more source