Results 61 to 70 of about 108,480 (310)

Long-term (35 years) cryopreservation of Echinococcus multilocularis metacestodes

, 2020
The metacestode of Echinococcus multilocularis is the etiological agent of alveolar echinococcosis. The metacestode stage used for research is maintained in rodents by serial passages. In order to determine whether cryopreservation of E.
Kronenberg, Philipp A, Alvarez Rojas, Cristian A; https://orcid.org/, Alvarez Rojas, Cristian A, Laurimäe, Teivi, Deplazes, Peter, Laurimäe, Teivi; https://orcid.org/, Ramp, Theodor W, Eckert, Johannes   +7 more
core   +1 more source

Cryopreservation and its clinical applications

Integrative Medicine Research, 2017
Cryopreservation is a process that preserves organelles, cells, tissues, or any other biological constructs by cooling the samples to very low temperatures.
Tae Hoon Jang, Sung Choel Park, Ji Hyun Yang, Jung Yoon Kim, J. Seok, U. Park, C. Choi, Sung Ryul Lee, Jin Han   +8 more
semanticscholar   +1 more source

CDK4/6 Inhibition Induces CD8+ T Cell Antitumor Immunity via MIF‐Induced Functional Orchestration of Tumor‐Associated Macrophages

Advanced Science, EarlyView.
CDK4/6 inhibition promotes CD8+ T cell expansion through tumor‐macrophage crosstalk by activating HIF‐1α and enhancing MIF‐CD44/CD74 signaling. This reprograms TAMs to boost MHC‐I antigen presentation, and CDK4/6 inhibitor‐trained M1 TAM supernatant therapy synergizes with low‐dose PD‐1 blockade to restore antitumor immunity.
Lin He, Yuzhong Peng, Lat‐lun Leong, Jingbo Zhou, Dongyang Tang, Weilu Wang, Xiaoran Wu, Josh haipeng Lei, Yongqin Ye, Yangyang Feng, Yunfeng Qiao, Xiangpeng Chu, Di Mu, Qi Zhao, Tzuming Liu, Yan Chen, Paul Kwonghang Tam, Chu‐Xia Deng   +17 more
wiley   +1 more source

Oocyte Cryopreservation - Current Scenario and Future Perspectives: A Narrative Review

Journal of Human Reproductive Sciences, 2021
Oocyte cryopreservation is a boon for women undergoing assisted reproductive technology. With the evolution in the technique of cryopreservation over the last three decades, there has been an exponential rise in the number of oocyte cryopreservation ...
H. Pai, R. Baid, N. Palshetkar, Arnav Pai, R. Pai, Rohan Palshetkar   +5 more
semanticscholar   +1 more source

rWTC‐MBTA Vaccine, Alone and Enhanced with Anti‐PD1, Elicits Immune Responses against CNS and Peripheral B‐Cell Lymphoma

Advanced Science, EarlyView.
An autologous whole‐tumor‐cell vaccine (rWTC‐MBTA) is evaluated in murine CNS lymphoma. Subcutaneous vaccination activates dendritic cells, broadens T‐cell priming, and drives lymphocyte trafficking to brain tumors, producing durable tumor control. Longitudinal bioluminescence and adoptive‐transfer assays verify CNS engagement. Combination with anti‐PD‐
Yaping Zhang, Juan Ye, Mitchell Sun, Samik Chakraborty, Alex Valenzuela, Shuran Chen, Qingfeng Xue, Wenyu Shi, Karel Pacak, Herui Wang, Zhengping Zhuang   +10 more
wiley   +1 more source

Cryopreservation method for Drosophila melanogaster embryos

Nature Communications, 2021
The development of a widely adopted cryopreservation method remains a major challenge in Drosophila research. Here we report a robust and easily implemented cryopreservation protocol of Drosophila melanogaster embryos.
Li Zhan, Mingang Li, T. Hays, J. Bischof
semanticscholar   +1 more source

Time‐Controlled Refrigerated Stem Cell Therapy Mitigates Scleroderma Fibrosis via Modulation of Mitochondrial Autophagy and Gut Metabolism

Advanced Science, EarlyView.
This study established an RT‐MSCs‐based therapeutic approach for scleroderma in mice. RT‐MSCs attenuated fibrosis by regulating mitochondrial autophagy and restored gut microbiota homeostasis. Metabolomic analyses confirmed recovery of key metabolites, and RT‐MSCs demonstrated favorable lesion targeting and safety profiles.
Xue Xia, Chenfei Kong, Xiaoming Zhao, Naixu Shi, Jinlan Jiang, Ping Li   +5 more
wiley   +1 more source

Induced encystment improves resistance to preservation and storage of Acanthamoeba castellanii

, 2008
Several conditions that allow the preservation, storage and rapid, efficient recovery of viable Acanthamoeba castellanii organisms were investigated. The viability of trophozoites (as determined by time to confluence) significantly declined over a period
Campbell, S. J., Roberts, C. W., Ingram, P.R., Campbell, S.J., Henriquez, F.L., Ingram, P. R., Henriquez, F. L., Roberts, C.W.   +7 more
core   +1 more source

The roles of reactive oxygen species and antioxidants in cryopreservation

Bioscience Reports, 2019
Cryopreservation has facilitated advancement of biological research by allowing the storage of cells over prolonged periods of time. While cryopreservation at extremely low temperatures would render cells metabolically inactive, cells suffer insults ...
J. Len, Wen Shuo Darius Koh, Shi-Xiong Tan   +2 more
semanticscholar   +1 more source

Adipocyte‐Derived Leptolin Enhances Energy Expenditure and Prevents Obesity

Advanced Science, EarlyView.
We identified a novel adipokine, which we named leptolin. In humans, leptolin levels in white adipose tissue were positively correlated with exercise and negatively associated with body mass index. We observed elevated leptolin in serum from athletes and lower leptolin in serum from obese individuals.
Jiarui Liu, Bingwei Wang, Zhijie Su, Xiaoxv Han, Miao He, Yun Zhao, Yujia Hou, Daotong Li, Weiguang Zhang, Lihua Qin, Ke Wang, Yanchun Li, Yi Yan, Siwang Yu, Xiaoshuai Huang, Tairan Yuwen, Jianyuan Luo, Ruimao Zheng   +17 more
wiley   +1 more source