Results 71 to 80 of about 79,865 (300)

Cryopreservation as part of the conservation strategy of Fragaria germplasm in Germany

In Germany, genetic resources of the genus Fragaria are predominantly represented by the 267 strawberry cultivars of the German Fruit Genebank, which is a decentralized network of different stakeholders, and the comprehensive wild species collection of ...
Wöhner, T., Flachowsky, H., Höfer, M.
core   +1 more source

Long-term (35 years) cryopreservation of Echinococcus multilocularis metacestodes [PDF]

, 2020
The metacestode of Echinococcus multilocularis is the etiological agent of alveolar echinococcosis. The metacestode stage used for research is maintained in rodents by serial passages. In order to determine whether cryopreservation of E.
Kronenberg, Philipp A, Alvarez Rojas, Cristian A; https://orcid.org/, Alvarez Rojas, Cristian A, Laurimäe, Teivi, Deplazes, Peter, Laurimäe, Teivi; https://orcid.org/, Ramp, Theodor W, Eckert, Johannes, Kronenberg PA, Deplazes P, Ramp TW, Laurimäe T, Alvarez Rojas CA, Eckert J   +13 more
core   +1 more source

Spontaneous Cyclic Levitation of Glycerol‐Water Droplets at Low Pressures

Advanced Science, EarlyView.
Under low pressure, a minute glycerol addition transforms the chaotic dynamics of freezing water droplets into cyclic levitation. Freeze‐concentration preserves liquid pathways that relax expansion‐induced stress, while freezing point depression weakens asymmetric vaporization recoil.
Chen Xu, Renjie Hua, Wei Ma, Fuqiang Chu, Dong Liang, Xueshi Li, Qirui Zhang, Yuan Dong, Xuehu Ma, Xiao Yan, Shuhuai Yao   +10 more
wiley   +1 more source

Polyproline: A Synthetic Polypeptide With Unique Properties

Angewandte Chemie, EarlyView.
Poly‐L‐proline is a unique polypeptide distinguished by its well‐defined secondary structures and unusual thermal behavior. This mini review highlights recent advances in the synthesis of poly‐L‐proline by N‐carboxyanhydride (NCA) polymerization, the formation of structurally defined architectures and nanostructures, and emerging strategies that ...
Rosanna Le Scouarnec, Josué M. Galindo, Colin Bonduelle, Andreas Heise   +3 more
wiley   +2 more sources

Endowing Flexibility of Alveolar Type II Cells to Recapitulate Development From Lung Bud to Alveoli

Advanced Science, EarlyView.
Adult human alveolar type 2 cells are chemically reprogrammed into expandable alveolar progenitor‐like cells that acquire bud tip‐like features during expansion and form organoids that mature into alveolar‐like structures. These organoids model influenza A virus infection and innate immune responses in vitro, while transplanted cells engraft ...
Hongqian Ma, Yuxin Zhang, Lujia Sun, Hong Jiang, Fan Li, Wenming Liu, Shenao Zhou, Mengting Wang, Weijian Huang, Xu Zhou, Caiyang Chen, Qiuqiu Zhang, Xiaoyan Jin, Feihan Yan, Hongping Wu, Yonghong Yin, Xinling Wang, Hexin Yan, Weifeng Yu   +18 more
wiley   +1 more source

Smart Bioinks for 4D Bioprinting: Requirements, Design, and Applications

Advanced Science, EarlyView.
Smart bioinks empower 4D‐bioprinted constructs to dynamically adapt and remodel in response to stimuli, effectively biomimicking native tissues. Artificial Intelligence (AI) and Machine Learning (ML) play a guiding role in their rational design by optimizing relevant properties.
Shangsi Chen, Jiahui Lai, Qiongjiao Zeng, Liangbin Zhou, Boguang Yang, Bin Zhang, Min Wang, Jiajing Zhou, Kieran Lau, Khoon S. Lim, Zhong Alan Li, Rocky S. Tuan   +11 more
wiley   +1 more source

Developmentally Guided Differentiation of Ventral Midbrain Dopaminergic Cells Validated in Proteopathy‐Based Parkinson's Disease Models

Advanced Science, EarlyView.
A developmentally guided protocol reproducibly specifies EN1‐positive ventral midbrain dopaminergic (vmDA) progenitors from human pluripotent stem cells and yields functional vmDA neurons. Optogenetic induction of α‐synuclein aggregation elicits selective degeneration of TH‐positive neurons in 2D cultures and 3D neurospheroids, while transplanted ...
Heechang Moon, Heejeong Kim, Elliot H. Lee, Hanseul Kim, Ji Hun Kim, Huisu Jeong, Jiwon Cheon, Ga Ram Jeong, Byoung Dae Lee, Jae‐Won Cho, Soo‐Jin Oh, Yunjong Lee, Yohan Oh   +12 more
wiley   +1 more source

Pathways to motherhood: A single-center retrospective study on fertility preservation and reproductive outcomes in patients with breast cancer

Journal of the Formosan Medical Association
Background: Breast cancer treatments often have negative effects on fertility, which pose challenges among patients who want to be parents in the future.
Chao-Ying Chen, Yu-Chiao Yi, Hwa-Fen Guu, Ya-Fang Chen, Hsiao-Fan Kung, Jui-Chun Chang, Li-Yu Chen, Chih-Chiang Hung, Ming-Jer Chen   +8 more
doaj   +1 more source

An overview of shoot tip cryopreservation

, 2020
The purpose of this chapter is to present an overview of the steps involved in plant shoot tip cryopreservation and to highlight some of the fundamental concepts behind these ...
Chen, K, Bettoni, Jean Carlos, Volk, G
core  

Induced encystment improves resistance to preservation and storage of Acanthamoeba castellanii [PDF]

, 2008
Several conditions that allow the preservation, storage and rapid, efficient recovery of viable Acanthamoeba castellanii organisms were investigated. The viability of trophozoites (as determined by time to confluence) significantly declined over a period
Campbell, S. J., Roberts, C. W., Ingram, P.R., Campbell, S.J., Henriquez, F.L., Ingram, P. R., Henriquez, F. L., Roberts, C.W.   +7 more
core   +1 more source