Results 51 to 60 of about 160,431 (399)
Molecular mechanisms of cell cryopreservation with polyampholytes studied by solid-state NMR
Polyampholytes are emerging macromolecular membrane non-penetrating cryoprotectants; however, the mechanism behind their cryopreservation remains unclear. Here, we investigated the mechanism using solid-state NMR spectroscopy.
K. Matsumura+4 more
semanticscholar +1 more source
Cryopreservation is considered the best technique for the safe, long-term conservation of embryogenic cultures. However, before integrating it into a somatic embryogenesis system, the influence of cryopreservation on the final production of plants should
Fatiha Bradaï, Carolina Sánchez-Romero
doaj +1 more source
Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing [PDF]
Byrsonima intermedia is a shrub from the Brazilian Cerrado with medicinal properties. The storage of biological material at ultra-low temperatures (-196°C) is termed cryopreservation and represents a promising technique for preserving plant diversity ...
Andre, E.+4 more
core +1 more source
Flow Cytometry of the Side Population: Tips & Tricks
Background: The Side Population (SP) has become an important hallmark for the definition of the stem cell compartment, especially in the detection of these cells and in their physical isolation by fluorescence-activated cell sorting (FACS).
Irene Sales-Pardo+9 more
doaj +1 more source
The most represented histotype of testicular cancer is the testicular germ-cell tumor (TGCT), both seminoma and non-seminoma. The pathogenesis of this cancer is poorly known.
Andrea Garolla+7 more
doaj +1 more source
Technologies for Vitrification Based Cryopreservation
Cryopreservation is a unique and practical method to facilitate extended access to biological materials. Because of this, cryopreservation of cells, tissues, and organs is essential to modern medical science, including cancer cell therapy, tissue ...
Mohammad Amini, James D. Benson
doaj +1 more source
Stepped vitrification technique for human ovarian tissue cryopreservation [PDF]
The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation.
Amorin, C.A.+9 more
core +1 more source
Step by step optimization of a sperm cryopreservation protocol for spotted wolffish (Anarhichas minor Olafsen, 1772) [PDF]
Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, low sperm volume with relatively low cell concentration and the lack of gametes synchronization (simultaneous availability of mature eggs and ...
Beirão, J.+3 more
core +2 more sources
Cryopreservation of Gametes and Embryos and Their Molecular Changes
The process of freezing cells or tissues and depositing them in liquid nitrogen at –196 °C is called cryopreservation. Sub-zero temperature is not a physiological condition for cells and water ice crystals represent the main problem since they induce ...
Enrique Estudillo+5 more
semanticscholar +1 more source
Abstract Rodent models of epilepsy remain the cornerstone of research into the mechanisms underlying genetic epilepsy. Reproducibility of experiments using these rodent models, occurring across a diversity of laboratories and commercial vendors, remains an issue impacting the cost‐effectiveness and scientific rigor of the studies performed.
Massimo Mantegazza+7 more
wiley +1 more source