Results 51 to 60 of about 160,431 (399)

Molecular mechanisms of cell cryopreservation with polyampholytes studied by solid-state NMR

open access: yesCommunications Materials, 2021
Polyampholytes are emerging macromolecular membrane non-penetrating cryoprotectants; however, the mechanism behind their cryopreservation remains unclear. Here, we investigated the mechanism using solid-state NMR spectroscopy.
K. Matsumura   +4 more
semanticscholar   +1 more source

Effect of Cryopreservation on the Ex Vitro Establishment of Olive Plants Regenerated via Somatic Embryogenesis

open access: yesPlants, 2021
Cryopreservation is considered the best technique for the safe, long-term conservation of embryogenic cultures. However, before integrating it into a somatic embryogenesis system, the influence of cryopreservation on the final production of plants should
Fatiha Bradaï, Carolina Sánchez-Romero
doaj   +1 more source

Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing [PDF]

open access: yes, 2015
Byrsonima intermedia is a shrub from the Brazilian Cerrado with medicinal properties. The storage of biological material at ultra-low temperatures (-196°C) is termed cryopreservation and represents a promising technique for preserving plant diversity ...
Andre, E.   +4 more
core   +1 more source

Flow Cytometry of the Side Population: Tips & Tricks

open access: yesCellular Oncology, 2006
Background: The Side Population (SP) has become an important hallmark for the definition of the stem cell compartment, especially in the detection of these cells and in their physical isolation by fluorescence-activated cell sorting (FACS).
Irene Sales-Pardo   +9 more
doaj   +1 more source

Role of Viral Infections in Testicular Cancer Etiology: Evidence From a Systematic Review and Meta-Analysis

open access: yesFrontiers in Endocrinology, 2019
The most represented histotype of testicular cancer is the testicular germ-cell tumor (TGCT), both seminoma and non-seminoma. The pathogenesis of this cancer is poorly known.
Andrea Garolla   +7 more
doaj   +1 more source

Technologies for Vitrification Based Cryopreservation

open access: yesBioengineering, 2023
Cryopreservation is a unique and practical method to facilitate extended access to biological materials. Because of this, cryopreservation of cells, tissues, and organs is essential to modern medical science, including cancer cell therapy, tissue ...
Mohammad Amini, James D. Benson
doaj   +1 more source

Stepped vitrification technique for human ovarian tissue cryopreservation [PDF]

open access: yes, 2019
The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation.
Amorin, C.A.   +9 more
core   +1 more source

Step by step optimization of a sperm cryopreservation protocol for spotted wolffish (Anarhichas minor Olafsen, 1772) [PDF]

open access: yes, 2020
Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, low sperm volume with relatively low cell concentration and the lack of gametes synchronization (simultaneous availability of mature eggs and ...
Beirão, J.   +3 more
core   +2 more sources

Cryopreservation of Gametes and Embryos and Their Molecular Changes

open access: yesInternational Journal of Molecular Sciences, 2021
The process of freezing cells or tissues and depositing them in liquid nitrogen at –196 °C is called cryopreservation. Sub-zero temperature is not a physiological condition for cells and water ice crystals represent the main problem since they induce ...
Enrique Estudillo   +5 more
semanticscholar   +1 more source

A companion to the preclinical common data elements for rodent genetic epilepsy models. A report of the TASK3‐WG1B: Paediatric and genetic models working group of the ILAE/AES joint translational TASK force

open access: yesEpilepsia Open, EarlyView., 2022
Abstract Rodent models of epilepsy remain the cornerstone of research into the mechanisms underlying genetic epilepsy. Reproducibility of experiments using these rodent models, occurring across a diversity of laboratories and commercial vendors, remains an issue impacting the cost‐effectiveness and scientific rigor of the studies performed.
Massimo Mantegazza   +7 more
wiley   +1 more source

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