Results 111 to 120 of about 48,476 (320)

Collection and freezing of equine epididymal spermatozoa [PDF]

, 2014
The epididymis and vas deferens store an important number of fertile spermatozoa called the extragonadal sperm reserves. These stored spermatozoa can be collected in an ultimate attempt to preserve viable spermatozoa of a critically ill or dying stallion.
Govaere, Jan, Hoogewijs, Maarten, Leemans, Bart, Roels, Kim, Van Soom, Ann, Ververs, Cyrillus   +5 more
core   +1 more source

Freeze-drying of ampicillin solid lipid nanoparticles using mannitol as cryoprotectant

, 2021
Farzaneh Alihosseini, Solmaz Ghaffari, Ali Reza Dabirsiaghi, Setareh Haghighat   +3 more
openalex   +1 more source

TRP canonical 4 and/or 5 channel inhibition reduces aversion‐ and increases reward‐responding in chronically stressed mice

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose Pharmacological inhibition of TRPC4 and/or TRPC5 channels reduces Pavlovian aversion memory in stressed mice and reduces amygdala reactivity to aversion in humans with depression. The aims of this mouse study were to improve understanding of these anxiolytic processes, determine whether there are corrective effects on ...
Giulia Poggi, Deniz Queisser, Alena Senn, Hannes Sigrist, Diana Kúkeľová, Lorraine Madur, Nagiua Cuomo‐Haymour, Mélisse Robert, Sophie Schmid, Adrián Portalés, Sophie Simard, Naguib Mechawar, Bastian Hengerer, Stefan Just, Christopher R. Pryce   +14 more
wiley   +1 more source

Practical Guide to Using Cryoprotectants in Biological Sample Preparation at Cryogenic temperature for Electron Microscopic Studies

Journal of Analytical Science and Technology, 2011
Cryo-fixation enables the preservation of the fine structures of intracellular organelles in a condition that is as close to their native state as possible compared with chemical fixation and room temperature processing.
A-Reum Je, Kyoung Hwan Lee, Heesu Chae, Soo Jin Kim, Ji-Hyun Lee, Yun Jung Choi, Mi Jeong Kim, Hyun Suk Jung, Hee-Seok Kweon   +8 more
doaj  

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant

Marine Drugs, 2017
Antifreeze proteins (AFPs) are biological antifreezes with unique properties, including thermal hysteresis(TH),ice recrystallization inhibition(IRI),and interaction with membranes and/or membrane proteins.
Hak Jun Kim, Jun Hyuck Lee, Young Baek Hur, Chang Woo Lee, Sun-Ha Park, Bon-Won Koo   +5 more
doaj   +1 more source

The Effect of Cryoprotectants and Storage Conditions on the Transfection Efficiency, Stability, and Safety of Lipid‐Based Nanoparticles for mRNA and DNA Delivery [PDF]

, 2023
Konstantinos N. Kafetzis, Natalia Papalamprou, Elisha McNulty, Kai Xin Thong, Yusuke Sato, Mironov Aa, Atul Purohit, Philip J. Welsby, Hideyoshi Harashima, Cynthia Yu‐Wai‐Man, Aristides D. Tagalakis   +10 more
openalex   +1 more source

Enzymatic synthesis of bioactive quinolones and (thio)coumarins by fungal type III polyketide synthases

The FEBS Journal, EarlyView.
Quinolones are valuable scaffolds for drug discovery but are rare in nature. Here, we show that two fungal enzymes, AthePKS and FerePKS, can generate 2‐quinolones and two additional heteroaromatic scaffolds. Using AthePKS, we designed an artificial enzymatic cascade towards an antimicrobial quinolone from a simple precursor and implemented it in E ...
Nika Sokolova, Angelina Osipyan, Lili Zhang, Matthew R. Groves, Sandy Schmidt, Kristina Haslinger   +5 more
wiley   +1 more source

A Review of the Material Characteristics, Antifreeze Mechanisms, and Applications of Cryoprotectants (CPAs) [PDF]

, 2021
Xiangjian Liu, Yuxin Pan, Fenglin Liu, Yongju He, Qubo Zhu, Zhaolin Liu, Xuehui Zhan, Songwen Tan   +7 more
openalex   +1 more source

Optimisation of freeze substitution protocols for the examination of malaria parasite structure by volumetric electron microscopy

Journal of Microscopy, EarlyView.
Abstract Malaria is one of the deadliest infectious diseases in the world, annually responsible for over 400,000 deaths. It is caused by parasites of the genus Plasmodium, which undergo remarkable structural changes during their development within different cells across various hosts.
Rachel Rachid, Camila Wendt, Wanderley de Souza, Kildare Miranda   +3 more
wiley   +1 more source

Cytoarchitecture and myeloarchitecture of the sheep auditory cortex

Journal of Anatomy, EarlyView.
This article describes the cyto‐ and myeloarchitecture of the sheep auditory cortex. It outlines a six‐layered cortical organization, with variations in cellular types and densities. The potential location of the primary auditory cortex is indicated by parvalbumin immunostaining.
Camille Pluchot, Mélody Morisse, Maryse Meurisse, Jean‐Marie Graïc, Elodie Chaillou, Scott A. Love   +5 more
wiley   +1 more source