Results 111 to 120 of about 57,341 (305)

Structure of a putative NTP pyrophosphohydrolase: YP_001813558.1 from Exiguobacterium sibiricum 255-15. [PDF]

, 2010
The crystal structure of a putative NTPase, YP_001813558.1 from Exiguobacterium sibiricum 255-15 (PF09934, DUF2166) was determined to 1.78 Å resolution. YP_001813558.1 and its homologs (dimeric dUTPases, MazG proteins and HisE-encoded phosphoribosyl ATP ...
Abdubek, Polat, Astakhova, Tamara, Axelrod, Herbert L, Carlton, Dennis, Chen, Connie, Chiu, Hsiu Ju, Clayton, Thomas, Das, Debanu, Deacon, Ashley M, Deller, Marc C, Duan, Lian, Elsliger, Marc André, Ernst, Dustin, Feuerhelm, Julie, Godzik, Adam, Grant, Joanna C, Grzechnik, Anna, Han, Gye Won, Hodgson, Keith O, Jaroszewski, Lukasz, Jin, Kevin K, Johnson, Hope A, Klock, Heath E, Knuth, Mark W, Kozbial, Piotr, Krishna, S Sri, Kumar, Abhinav, Lam, Winnie W, Lesley, Scott A, Marciano, David, McMullan, Daniel, Miller, Mitchell D, Morse, Andrew T, Murzin, Alexey G, Nigoghossian, Edward, Okach, Linda, Reyes, Ron, Rife, Christopher L, Sefcovic, Natasha, Tien, Henry J, Trame, Christine B, van den Bedem, Henry, Weekes, Dana, Wilson, Ian A, Wooley, John, Xu, Qingping, Yeates, Todd O   +46 more
core   +2 more sources

Cryoprotectant‐free vitrification of spermatozoa: Fish as a model of human

Andrologia, 2018
Post‐thawing motility of spermatozoon, which is directly correlated with the integrity of mitochondrion, is the main parameter for evaluation of respective cryopreservation treatments.
V. Isachenko, R. Sánchez, G. Rahimi, P. Mallmann, E. Isachenko, Markus Merzenich   +5 more
semanticscholar   +1 more source

Stabilized Extracellular Vesicle Formulations for Inhalable Dry Powder Development

Small, EarlyView.
Extracellular vesicles (EVs) hold promise for chronic obstructive pulmonary disease (COPD) treatment but face challenges with storage and delivery. This study demonstrates the stabilization of lung fibroblast‐derived EVs using inulin, enabling freeze‐drying and spray‐drying.
Eva M. Jansen, Luke van der Koog, Robin A.B. Elferink, Karim Rafie, Anika Nagelkerke, Reinoud Gosens, Henderik W. Frijlink, Wouter L.J. Hinrichs   +7 more
wiley   +1 more source

Mechanosensitive Cell‐Laden Shape‐Memory Scaffolds Engineered via Cryogelation

Small Structures, EarlyView.
GelMA‐based shape‐memory scaffolds encapsulating human adipose‐derived stem cells are fabricated via cryogelation, showing rapid recovery and high cell viability. Under cyclic compression, they promoted mechanotransduction gene expression, and composite formulations (collagen/GelMA, dECM/GelMA) retained robust shape‐memory, highlighting their promise ...
JaeYoon Lee, ByungJoon Choi, Hanjun Hwangbo, GaEun Heo, GeunHyung Kim   +4 more
wiley   +1 more source

Effect of different cryoprotectants on the structural preservation of follicles in frozen zebu bovine (Bos indicus) ovarian tissue [PDF]

, 2004
Cryopreservation of ovarian tissue is a new and promising technique for germ-line storage. The objective of this study was to evaluate the effect of four cryoprotectants (at two concentrations each) on the preservation of zebu bovine preantral follicles ...
Báo, Sônia Nair, Kacinskis, Mirella Ávila, Lopes, Luiz Henrique R, Lucci, Carolina Madeira, Rumpf, Rodolfo   +4 more
core   +1 more source

Natural deep eutectic systems, an emerging class of cryoprotectant agents

Cryobiology, 2021
A. R. Jesus, Liane Meneses, A. Duarte, Alexandre Paiva   +3 more
semanticscholar   +1 more source

Complete protection against cryodamage of cryopreserved whole bovine and human ovaries using DMSO as a cryoprotectant

Journal of Assisted Reproduction and Genetics, 2017
PurposeThis study aims to determine the optimal cryopreservation protocol for whole ovaries intended for preservation of fertility in women.MethodsWe investigated the optimal cryopreservation procedure for whole ovaries in a bovine model.
J. R. Westphal, R. Gerritse, D. Braat, C. Beerendonk, R. Peek   +4 more
semanticscholar   +1 more source

Crystal structure of a short‐chain dehydrogenase from Brucella ovis with apo and coenzyme NAD+‐bound protomer chains

Acta Crystallographica Section F, EarlyView.
This article describes the structural characterization of a short‐chain dehydrogenase from the pathogenic bacterium Brucella ovis. In this manuscript, two structures are described: the structure of the apo protein and a structure containing the coenzyme NAD+.Short‐chain dehydrogenases (SDRs) are a family of NAD(P)‐dependent enzymes involved in redox ...
Sean P. Zupko, Amelia T. Konstanty, Steve J. Mayclin, Ryan Choi, Dmitry Serbzhinskiy, Emily Robles, Victoria Moses, Lynn K. Barrett, Wesley C. Van Voorhis, Tom E. Edwards, Peter J. Myler, Andrew T. Torelli, Jarrod B. French, Katherine A. Hicks   +13 more
wiley   +1 more source

Research Concerning Use of Long-Term Preservation Techniques for Microorganisms

Scientific Papers Animal Science and Biotechnologies, 2023
From the large number of methods used for storage of microorganisms, the more effective methods are long-term cryopreservation and lyophilization. The temperature change rate, controls transport of water around cell membranes and indirectly likelihood of
Adriana Criste, Mihaela Giuburuncă, Octavian Negrea, Sorin Dan, Marius Zăhan   +4 more
doaj  

Crystallization, data collection and data processing of maltose-binding protein (MalE) from the phytopathogen Xanthomonas axonopodis pv. citri [PDF]

, 2009
Maltose-binding protein is the periplasmic component of the ABC transporter responsible for the uptake of maltose/maltodextrins. The Xanthomonas axonopodis pv.
Balan, A., Barbosa, J. A. R. G., Ferreira, L. C. S., Souza, C. S., Thomas, L.   +4 more
core   +3 more sources