Advancing urine‐derived stem cells: Cryopreservation validation and sex‐specific metabolism
Urine‐derived stem cells (UDSC) are a non‐invasive, easily accessible source of stem cells with robust differentiation potential. We developed a cryopreservation protocol for UDSC isolated from healthy donors, assessing viability, apoptosis/necrosis, metabolic profile and differentiation capacity.
Gonçalo José Martins Afonso +7 more
wiley +1 more source
High-Efficiency Cryopreservation of Silver Pomfret Sperm: Protocol Development and Cryodamage Assessment. [PDF]
Zhang M +10 more
europepmc +1 more source
Development of a highly active engineered PETase enzyme for polyester degradation
Polyethylene terephthalate (PET) accounts for approximately 6% of global plastic production and is a major contributor to plastic pollution. Enzymatic recycling offers a promising solution, but current PET‐degrading enzymes often lack sufficient thermostability and catalytic efficiency. Through in silico design, we engineered a more robust variant, LCC‐
Shapla Bhattacharya +6 more
wiley +1 more source
Medium-term storage of platelet-derived orthobiologics: a feasible alternative for equine practice. [PDF]
Seidel SRT +5 more
europepmc +1 more source
Nucleotide sugar short‐chain dehydrogenases/reductases (NS‐SDRs) are involved in pseudomurein and capsular polysaccharide formation in methanogenic Archaea. Methanothermobacter thermautotrophicus possesses several NS‐SDRs labelled Mth375, Mth380, Mth373, Mth631 and Mth1789.
Vincenzo Carbone +4 more
wiley +1 more source
Protocol for fixation and staining of postmortem human brain tissue optimized for focused ion beam-scanning electron microscopy. [PDF]
Maier MJ +3 more
europepmc +1 more source
ABSTRACT Aim This study aimed to evaluate whether human dental pulp stem cells (DPSCs), after long‐term biobanking (7–8 years), retain their pro‐angiogenic properties and can be used to engineer vascularised tissues, addressing their potential for clinical translation in regenerative dentistry.
Shuntaro Yamada +10 more
wiley +1 more source
Design of Ready-to-Use "Ball-in-Ball" <i>Staphylococcus aureus</i> Microsphere Based on Novel Cryoprotectant and Drop Freeze-Drying Technology: Effective Preservation and Application. [PDF]
Wang Z +8 more
europepmc +1 more source
Introduction Bee pollen, a natural product rich in polyphenols, exhibits remarkable antioxidant, anti‐inflammatory, and hepatoprotective properties. Aim This study was aimed at evaluating the hepatoprotective effects of solid lipid nanoparticles (SLNs) loaded with bee pollen.
Khashayar Sanemar +4 more
wiley +1 more source
Ultrasonic rewarming of cryopreserved alginate encapsulated liver spheroids. [PDF]
Xu R +4 more
europepmc +1 more source

