Somatic cell reprogramming for Parkinson's disease treatment
Ibrain, Volume 11, Issue 1, Page 59-73, Spring 2025.The fundamental purpose of cell reprogramming to treat Parkinson's disease is to generate dopaminergic neurons (DAN) and do transplantation. There are two ways to accomplish this. One method is to induce cells into induced DA neurons (iDAN) directly or to induce cells into induced pluripotent stem cells and ultimately into iDAN in vitro. Another option
Xiaozhuo Li, Kevin Fang, Fengping Wang
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Transitioning from a research protocol to a scalable applied pathway for Xenopus laevis sperm cryopreservation at a national stock center: The effect of cryoprotectants [PDF]
, 2023 Lucía Arregui +2 more
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Ribosomal RNA operon copy number: A trait‐informed framework to close the microbial cultivation gap
iMetaOmics, EarlyView.The vast majority of microbes remain uncultured, constraining functional validation and limiting microbiome‐based applications. We show that ribosomal RNA operon (rrn) copy number—a stable, phylogenetically conserved genomic trait—is strongly correlated with cultivation outcomes across ecosystems.
Yuxiao Huang +8 more
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ChemistryOpen, EarlyView.X‐ray crystallographic analysis of super‐folder green fluorescent protein (sfGFP), a common reporter protein, displaying p‐(phenylazo)‐L‐phenylalanine (Pap) at amino acid position 39 on its surface in complex with α‐cyclodextrin (α‐CD) reveals an unexpected conformation of the photoswitchable side chain and illustrates its light‐dependent ...
Andreas Eichinger +4 more
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Pengaruh Jenis Dan Lama Perendaman Krioprotektan Terhadap Viabilitas Benih Rosella (Hibiscus Sabdariffa L.) Secara Kriopreservasi [PDF]
, 2015 Cryopreservation is a technique for long-term storage nuftah plasma. In this technique cells and meristem or other parts of plants were frozen and stored in liquid nitrogen at a temperature -196°C.
Harahap, A. A. (Ahmad) +2 more
core
Preservation of bovine preantral follicle viability and ultra-structure after cooling and freezing of ovarian tissue [PDF]
, 2008 Bovine preantral follicles within ovarian fragments were exposed and cryopreserved in absence or presence of 1.5 M glycerol (GLY), ethylene glycol (EG), propanediol (PROH) or dimethyl sulfoxide (DMSO), undergoing a previous cooling at 20 °C for 1 h ...
Báo, Sônia Nair +9 more
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Structural Analysis and Inhibitor Modeling of Bacterioferritin From Brucella abortus
Proteins: Structure, Function, and Bioinformatics, EarlyView.ABSTRACT Iron homeostasis in various pathogenic bacteria is regulated by bacterioferritins (Bfr) which function to store Fe3+ and release Fe2+ as needed for metabolic processes. The Bfr structure consists of 18 kDa subunits in which dimer pairs bind a heme molecule and are assembled into a highly symmetrical 24‐meric spherical structure with an ...
Lijun Liu +9 more
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NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEON
Юг России: экология, развитие, 2016 The aim of the research is to increase the survivability of reproductive cells of sturgeon at cryopreservation and developing reliable technology suitable for use on an industrial scale.Methods.
E. N. Ponomareva +3 more
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Structure of 3,4-Dihydroxy-2-butanone 4-Phosphate Synthase from Methanococcus jannaschii in Complex with Divalent Metal Ions and the Substrate Ribulose 5-Phosphate: implications for the catalytic mechanism [PDF]
, 2003 Skeletal rearrangements of carbohydrates are crucial for many biosynthetic pathways. In riboflavin biosynthesis ribulose 5-phosphate is converted into 3,4-dihydroxy-2-butanone 4-phosphate while its C4 atom is released as formate in a sequence of metal ...
Bacher, Adelbert +5 more
core
Proteins: Structure, Function, and Bioinformatics, EarlyView.ABSTRACT We present the first x‐ray crystallographic structural evidence of an archaeal DNA ligase showing the AMP covalent adduct together with further cofactor hydrolysis, capturing a transient intermediary in the first step of the ligation reaction, triggered by the pyrophosphate hydrolysis.
A. X. Quintana‐Armas +3 more
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