Results 11 to 20 of about 45,860 (139)

N‐acetylcysteine attenuates oxidative stress‐mediated cell viability loss induced by dimethyl sulfoxide in cryopreservation of human nucleus pulposus cells: A potential solution for mass production

open access: yesJOR SPINE, Volume 5, Issue 4, December 2022., 2022
N‐acetylcysteine attenuates DMSO induced oxidative stress mediated viability loss in NP cells. Abstract Background Cell therapy is considered a promising strategy for intervertebral disc (IVD) regeneration. However, cell products often require long‐term cryopreservation, which compromises cell viability and potency, thus potentially hindering ...
Shota Tamagawa   +11 more
wiley   +1 more source

Colloidal Polyelectrolyte Complexes from Hyaluronic Acid: Preparation and Biomedical Applications

open access: yesSmall, Volume 18, Issue 51, December 22, 2022., 2022
Hyaluronic acid (HA) is a very promising polysaccharide for biomedical applications. This review provides a comprehensive overview of the development of nanoparticulate systems by HA‐based polyelectrolyte complexation. The main achievements and applications for the delivery of active substances or the construction of multifunctional platforms are ...
Huu Van Le, Didier Le Cerf
wiley   +1 more source

Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm

open access: yesReproduction in Domestic Animals, Volume 58, Issue 1, Page 184-188, January 2023., 2023
Abstract This study evaluated the bioenergetic map of mitochondria metabolism in cryopreserved bovine sperm. The detected oligomycin‐sensitive basal respiration supported ATP production; frozen–thawed spermatozoa were found to have a coupling efficiency higher than 0.80. Cell respiration, however, was not stimulated by the protonophoric action of FCCP,
Cristina Algieri   +7 more
wiley   +1 more source

Insights into Chemical Interactions and Related Toxicities of Deep Eutectic Solvents with Mammalian Cells Observed Using Synchrotron Macro–ATR–FTIR Microspectroscopy

open access: yesBiophysica, 2023
Deep eutectic solvents (DESs) and ionic liquids (ILs) are highly tailorable solvents that have shown a lot of promise for a variety of applications including cryopreservation, drug delivery, and protein stabilisation.
Saffron J. Bryant   +8 more
doaj   +1 more source

Carboxylated Poly-l-Lysine as a Macromolecular Cryoprotective Agent Enables the Development of Defined and Xeno-Free Human Sperm Cryopreservation Reagents

open access: yesCells, 2021
In human sperm cryopreservation, test yolk buffer and human serum albumin have been used as permeating macromolecular-weight cryoprotectants. In clinical reproductive medicine, human serum albumin is frequently used because of low risks of zoonoses and ...
Hiroki Takeuchi   +13 more
doaj   +1 more source

Caffeic Acid Phenethyl Ester Alleviates Cryodamage To Lung Cancer Cells During Cryopreservation

open access: yesHittite Journal of Science and Engineering, 2021
Cryopreservation is widely used technique for long-term preservation of viable cells at low temperature. In this process, considering the effects of cryodamage on cells, the application of safe and efficient cryoprotective agents is very important ...
Ezgi Avşar Abdik
doaj   +1 more source

Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges

open access: yesAnimals, 2022
Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro   +4 more
doaj   +1 more source

Interplay of vitrification and ice formation in a cryoprotectant aqueous solution at low temperature [PDF]

open access: yes, 2021
The proneness of water to crystallize is a major obstacle to understanding its putative exotic behavior in the supercooled state. It also represents a strong practical limitation to cryopreservation of biological systems. Adding some concentration of glycerol, which has a cryoprotective effect preventing to some degree water crystallization, has been ...
arxiv   +1 more source

Rapid joule heating improves vitrification based cryopreservation

open access: yesNature Communications, 2022
Lower concentrations of toxic cryoprotective agents require rapid and scalable rewarming techniques. Here, the authors report on a joule heating–based platform where samples are rapidly rewarmed by contact with a voltage pulsed electrical conductor and ...
Li Zhan   +5 more
doaj   +1 more source

Improving of Robotic Virtual Agent's errors that are accepted by reaction and human's preference [PDF]

open access: yesInternational Conference on Social Robotics, Dec 2023, 2023
One way to improve the relationship between humans and anthropomorphic agents is to have humans empathize with the agents. In this study, we focused on a task between an agent and a human in which the agent makes a mistake. To investigate significant factors for designing a robotic agent that can promote humans empathy, we experimentally examined the ...
arxiv   +1 more source

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