Caffeic Acid Phenethyl Ester Alleviates Cryodamage To Lung Cancer Cells During Cryopreservation
Cryopreservation is widely used technique for long-term preservation of viable cells at low temperature. In this process, considering the effects of cryodamage on cells, the application of safe and efficient cryoprotective agents is very important ...
Ezgi Avşar Abdik
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Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges
Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro+4 more
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Rapid joule heating improves vitrification based cryopreservation
Lower concentrations of toxic cryoprotective agents require rapid and scalable rewarming techniques. Here, the authors report on a joule heating–based platform where samples are rapidly rewarmed by contact with a voltage pulsed electrical conductor and ...
Li Zhan+5 more
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Interactions of cryoprotective agents with phospholipid membranes - A Langmuir monolayer study [PDF]
The influence of four common cryoprotectants (dimethyl sulfoxide, glycerol, ethylene glycol and dimethylformamide) on monolayers of four common phospholipids (DPPC, DOPC, POPC and POPE) have been studied using Langmuir isotherms and monolayer insertion experiments. The cryoprotectant concentrations were chosen to be directly relevant to cryoprotection.
Rekha Raju+2 more
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Optimization of Adipose Tissue Cryopreservation Techniques in a Murine Model
Background:. The aim of this study was to develop an adipose tissue (AT) cryopreservation protocol that is effective, simple, and maintains the functionality and viability of AT after thawing and transplantation. Methods:.
María Eloísa Villaverde-Doménech, PhD+5 more
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A simple and effective protocol for cryopreservation of germplasm of the bull kelp, Nereocystis luetkeana (Phaeophyceae). [PDF]
Abstract Kelps are large brown seaweeds that can form three‐dimensional underwater forests that provide food and habitat for a wide diversity of marine organisms. They also provide a wealth of ecosystem services to humans and may be able to help combat climate change through blue carbon.
Coleman LJM+3 more
europepmc +2 more sources
Cryopreservation practices in clinical and preclinical iPSC-based cell therapies: Current challenges and future directions. [PDF]
Abstract Induced pluripotent stem cells (iPSCs) offer significant therapeutic potential, but cryopreservation challenges, particularly the reliance on cytotoxic Dimethyl Sulfoxide (Me2SO), hinder their clinical application. This review examines current cryopreservation practices in clinical and preclinical iPSC‐based therapies, highlighting the ...
Dobruskin M, Toner G, Kander R.
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Toxicity of cryoprotective agents at 30°
Abstract The toxicity of four cryoprotective agents (glycerol, dimethylsulfoxide, dextran, magnesium ion) as a function of time was assessed at 30° on isolated rabbit atria. Glycerol, 2·0 M, in Ringer solution killed all atria in less than 30 min. Atria tolerated 2·1 M dimethylsulphoxide in Ringer for 1 hr, 0·7 M glycerol in Ringer for 4
Ben R. Clower+2 more
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New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification
Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of the protocol is necessary to reach clinical efficiency.
Daniel Angel-Velez+8 more
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Fatty Acid Profiling of Moina sp. Preserved in Cryoprotective Agents at Low Temperature
Highlight Research • Saturated fatty acid (SFA), polyunsaturated fatty acid (PUFA), ∑ ω6 and ∑ ω3 of Moina sp. preserved with 5, 10 and 20% GLY decreased with prolong storage. • FA of Moina sp.
Dr. Nurul Ulfah Karim+3 more
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