Results 31 to 40 of about 3,438 (209)

Caffeic Acid Phenethyl Ester Alleviates Cryodamage To Lung Cancer Cells During Cryopreservation

open access: yesHittite Journal of Science and Engineering, 2021
Cryopreservation is widely used technique for long-term preservation of viable cells at low temperature. In this process, considering the effects of cryodamage on cells, the application of safe and efficient cryoprotective agents is very important ...
Ezgi Avşar Abdik
doaj   +1 more source

Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges

open access: yesAnimals, 2022
Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro   +4 more
doaj   +1 more source

Rapid joule heating improves vitrification based cryopreservation

open access: yesNature Communications, 2022
Lower concentrations of toxic cryoprotective agents require rapid and scalable rewarming techniques. Here, the authors report on a joule heating–based platform where samples are rapidly rewarmed by contact with a voltage pulsed electrical conductor and ...
Li Zhan   +5 more
doaj   +1 more source

Interactions of cryoprotective agents with phospholipid membranes - A Langmuir monolayer study [PDF]

open access: greenChemistry and Physics of Lipids, 2020
The influence of four common cryoprotectants (dimethyl sulfoxide, glycerol, ethylene glycol and dimethylformamide) on monolayers of four common phospholipids (DPPC, DOPC, POPC and POPE) have been studied using Langmuir isotherms and monolayer insertion experiments. The cryoprotectant concentrations were chosen to be directly relevant to cryoprotection.
Rekha Raju   +2 more
openalex   +5 more sources

Optimization of Adipose Tissue Cryopreservation Techniques in a Murine Model

open access: yesPlastic and Reconstructive Surgery, Global Open, 2021
Background:. The aim of this study was to develop an adipose tissue (AT) cryopreservation protocol that is effective, simple, and maintains the functionality and viability of AT after thawing and transplantation. Methods:.
María Eloísa Villaverde-Doménech, PhD   +5 more
doaj   +1 more source

A simple and effective protocol for cryopreservation of germplasm of the bull kelp, Nereocystis luetkeana (Phaeophyceae). [PDF]

open access: yesJ Phycol
Abstract Kelps are large brown seaweeds that can form three‐dimensional underwater forests that provide food and habitat for a wide diversity of marine organisms. They also provide a wealth of ecosystem services to humans and may be able to help combat climate change through blue carbon.
Coleman LJM   +3 more
europepmc   +2 more sources

Cryopreservation practices in clinical and preclinical iPSC-based cell therapies: Current challenges and future directions. [PDF]

open access: yesBiotechnol Prog
Abstract Induced pluripotent stem cells (iPSCs) offer significant therapeutic potential, but cryopreservation challenges, particularly the reliance on cytotoxic Dimethyl Sulfoxide (Me2SO), hinder their clinical application. This review examines current cryopreservation practices in clinical and preclinical iPSC‐based therapies, highlighting the ...
Dobruskin M, Toner G, Kander R.
europepmc   +2 more sources

Toxicity of cryoprotective agents at 30°

open access: yesJournal of Pharmacy and Pharmacology, 1968
Abstract The toxicity of four cryoprotective agents (glycerol, dimethylsulfoxide, dextran, magnesium ion) as a function of time was assessed at 30° on isolated rabbit atria. Glycerol, 2·0 M, in Ringer solution killed all atria in less than 30 min. Atria tolerated 2·1 M dimethylsulphoxide in Ringer for 1 hr, 0·7 M glycerol in Ringer for 4
Ben R. Clower   +2 more
openaire   +2 more sources

New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification

open access: yesAnimals, 2021
Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of the protocol is necessary to reach clinical efficiency.
Daniel Angel-Velez   +8 more
doaj   +1 more source

Fatty Acid Profiling of Moina sp. Preserved in Cryoprotective Agents at Low Temperature

open access: yesJurnal Ilmiah Perikanan dan Kelautan, 2021
Highlight Research • Saturated fatty acid (SFA), polyunsaturated fatty acid (PUFA), ∑ ω6 and ∑ ω3 of Moina sp. preserved with 5, 10 and 20% GLY decreased with prolong storage. • FA of Moina sp.
Dr. Nurul Ulfah Karim   +3 more
doaj   +1 more source

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