Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges
Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro +4 more
doaj +1 more source
Rapid joule heating improves vitrification based cryopreservation
Lower concentrations of toxic cryoprotective agents require rapid and scalable rewarming techniques. Here, the authors report on a joule heating–based platform where samples are rapidly rewarmed by contact with a voltage pulsed electrical conductor and ...
Li Zhan +5 more
doaj +1 more source
Yeast Exoglycoproteins Produced Under NaCl-Stress Conditions as Efficient Cryoprotective Agents
Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. Glycoproteins secreted by the strain Dipodascus australiensis into growth medium
Breierova Emilia
doaj +1 more source
Cryopreservation: Methods, equipment and critical concerns [PDF]
The technique of cryopreservation aims to maintain the biological samples in a metabolic suspension state, by which all chemical, physical and biological changes are diminished.
Herrmann, J +3 more
core +1 more source
Optimization of Adipose Tissue Cryopreservation Techniques in a Murine Model
Background:. The aim of this study was to develop an adipose tissue (AT) cryopreservation protocol that is effective, simple, and maintains the functionality and viability of AT after thawing and transplantation. Methods:.
María Eloísa Villaverde-Doménech, PhD +5 more
doaj +1 more source
New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification
Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of the protocol is necessary to reach clinical efficiency.
Daniel Angel-Velez +8 more
doaj +1 more source
Pressure in isochoric systems containing aqueous solutions at subzero Centigrade temperatures. [PDF]
ObjectivePreservation of biological materials at subzero Centigrade temperatures, cryopreservation, is important for the field of tissue engineering and organ transplantation.
Năstase, Gabriel +3 more
core +3 more sources
The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue
Abstract Background Long-term preservation of adipose tissue is crucial for clinical applications. Researchers should consider both efficiency and biosafety when choosing a cryoprotective agent (CPA) for adipose tissue preservation.
Pei-Qi Zhang +7 more
openaire +3 more sources
Aquaglyceroporins are known as channel proteins, and are able to transport water and small neutral solutes. In this study, we evaluate the effect of exposure of in vitro matured bovine oocytes to hyperosmotic solutions containing ethylene glycol (EG ...
Tania García-Martínez +8 more
doaj +1 more source
Preservation of bovine preantral follicle viability and ultra-structure after cooling and freezing of ovarian tissue [PDF]
Bovine preantral follicles within ovarian fragments were exposed and cryopreserved in absence or presence of 1.5 M glycerol (GLY), ethylene glycol (EG), propanediol (PROH) or dimethyl sulfoxide (DMSO), undergoing a previous cooling at 20 °C for 1 h ...
Báo, Sônia Nair +9 more
core +1 more source

