s to assess different CPAs on stallion semen cryopreservation. Skim milk (SM) and Dimitropoulos(DV) were the extenders used in this study; each was added by glycerol (Gly), combination of ethyleneglycol-glycerol (EG+Gly) or dimethilformamide (DMF).
R.I. Arifiantini +3 more
doaj +1 more source
Cryopreservation of swine ovarian tissue : effect of different cryoprotectants on the structural preservation of preantral follicle oocytes [PDF]
The present study aimed to test different cryoprotectants on cryopreservation of pig ovarian tissue. Pig ovaries (n = 3) were collected at a local slaughterhouse. From each ovary, ten cortex samples were taken.
Amorima, C. A. +6 more
core +1 more source
Cryopreservation of cells using defined serum-free cryoprotective agents
For regenerative purposes, there is a high demand for viable and active cells. A big issue is to have enough viable cells available at any given time. One solution is cryopreservation.
Volbers Jan-Cedric +3 more
doaj +1 more source
Fatty Acid Profiling of Moina sp. Preserved in Cryoprotective Agents at Low Temperature
Highlight Research • Saturated fatty acid (SFA), polyunsaturated fatty acid (PUFA), ∑ ω6 and ∑ ω3 of Moina sp. preserved with 5, 10 and 20% GLY decreased with prolong storage. • FA of Moina sp.
Dr. Nurul Ulfah Karim +3 more
doaj +1 more source
Stepped vitrification technique for human ovarian tissue cryopreservation [PDF]
The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation.
Amorin, C.A. +9 more
core +1 more source
Effects of different antioxidants on cryopreservation of Jinhua boar sperm
To compare the effects of different antioxidant concentrations of glutathione (GSH), green tea polyphenols (GTPs), vitamin E (VE) and superoxide dismutase (SOD) on cryopreservation of Jinhua boar sperm, freezing extenders were supplemented with the GSH ...
WANG Zheng-guang +4 more
doaj +1 more source
Effect of diluent variation on cryopreservation of large yellow croaker Larimichthys crocea
The aim of this research was to investigate different factors, including cryoprotective agents (CPAs), diluents, dilution ratios, equilibrium times, freezing rates, and thawing methods to optimize cryopreservation ...
Han Kyu Lim +4 more
doaj +1 more source
Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions
Vitrification of aqueous cryoprotectant mixtures is essential in cryopreservation of proteins and other biological samples. We report systematic measurements of critical cryoprotective agent (CPA) concentrations required for vitrification during plunge ...
Alsaied, Osama A. +3 more
core +1 more source
Toxicity of cryoprotective agents at 30°
Abstract The toxicity of four cryoprotective agents (glycerol, dimethylsulfoxide, dextran, magnesium ion) as a function of time was assessed at 30° on isolated rabbit atria. Glycerol, 2·0 M, in Ringer solution killed all atria in less than 30 min. Atria tolerated 2·1 M dimethylsulphoxide in Ringer for 1 hr, 0·7 M glycerol in Ringer for 4
Armand M Karow +2 more
openaire +1 more source
Cryopreservation and its clinical applications
Cryopreservation is a process that preserves organelles, cells, tissues, or any other biological constructs by cooling the samples to very low temperatures.
Tae Hoon Jang +8 more
doaj +1 more source

