Results 71 to 80 of about 3,438 (209)

Lyophilised Reverse Transcriptase and Polymerase for Point‐of‐Care Diagnostics of SARS‐CoV‐2

Microbial Biotechnology, Volume 18, Issue 7, July 2025.
Design, expression and purification of Klentaq polymerase, Taq polymerase and MMLV reverse transcriptase to prepare a custom‐made amplification mix. To enable cold‐free storage, the amplification mix was lyophilised. The prepared lyophilised amplification mix was used on a point of care device to efficiently detect SARS‐CoV‐2 RNA.
Natalie Mutter, Barbara Posch, Yasmin Gillitschka, Nadezhda Kataeva, Birgit Michalek, Daniel Lager, Filip Staniszewski, Alexandra Schilder, Lidiya Osinkina, Maximilian Westenthanner, Joachim Stehr, Federico Buersgens, Johannes R. Peham   +12 more
wiley   +1 more source

Supercooling of Alaskan Beetle Larvae as a Winter Survival Strategy

Small Science, Volume 5, Issue 6, June 2025.
Dehydrated Alaskan beetle larvae in Fairbanks, Alaska, adhere to the bark of trees that are embedded in ice in their natural winter microhabitat (bottom). This enables their bodies to deeply supercool and avoid freezing, in contrast to their fully hydrated summer form (top).
Chris J. Benmore, Leighanne C. Gallington, Henry Vu, John G. Duman, Brian M. Barnes, Todd L. Sformo   +5 more
wiley   +1 more source

Fertilizing capacity of cryopreserved sperm of Chirostoma jordani (Woolman, 1894)

Fisheries and Aquatic Sciences
The genus Chirostoma is endemic from the Mesa Central of Mexico. It is conformed of 18 species and six subspecies. Five species are in some category of risk, because of this, Chirostoma jordani is an excellent model species to implement biotechnologies ...
Bustamante-González Jesús Dámaso, Gutiérrez-Díaz Dulce Leticia, Baca-Alejo Judith Sarai, Figueroa-Lucero Gerardo, Arenas-Ríos Edith, Hernández-Rubio María Cecilia, Ávalos-Rodríguez Alejandro   +6 more
doaj   +1 more source

Evaluation of Ethylene Glycol as a Cryoprotectant in the Sperm Cryopreservation of Trans-andean Shovelnose Catfish (Sorubim Cuspicaudus, Pimelodidae)

Acta Biológica Colombiana, 2014
The catfish Sorubim cuspicaudus cryopreservation semen was evaluated using three levels (5, 10, 15%) of ethylene glycol (ETG). Males (n = 13) undergoing spermiation and in final maturation females (n = 6) were induced with 0.4 ml Ovaprim®/Kg, after 12 ...
Victor Julio Atencio García, Maria Dorado, Emilio Navarro, Francisco Pérez, Briner Herrera, Jorge Movilla, José Espinosa-Araujo   +6 more
doaj   +1 more source

Osmotic Injury to Porcine Oocytes during Cryoprotective Agents Addition and Removal Processes

Zhileng xuebao, 2017
Oocyte cryopreservation has become a practical tool in assisted reproductive technology and fertility preservation. Cryoprotective agents (CPAs) addition and removal are essential steps during oocytes cryopreservation, however, the processes of CPAs ...
Yang Yun, Zhou Xinli, Dai Jianjun, Zhang Defu, Yi Xingyue, Teng Yun, Tao Leren   +6 more
doaj  

Effects of different cryoprotective agents on ram sperm morphology and DNAintegrity

Theriogenology, 2010
This study investigates the effects of glycerol, 1,2 propanediol, sucrose, and trehalose on post-thaw motility, morphology, and genome integrity of Awassi ram semen. Ejaculates of thick consistency with rapid wave motion (>+++) and >70% initial motility were pooled.
Nur, Z., Zik, B., Ustuner, B., Sagirkaya, H., Ozguden, C. G.   +4 more
openaire   +5 more sources

Assessment of the cryoprotective effects of fetal bovine serum (FBS) and trehalose on the viability rate of caprine spermatogonial stem cells (SSCs)

Fiyz̤, 2021
Background: Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, because they have the capacity of self-renewal, and differentiation into spermatozoa. Freezing is the most common long-term preservation approach for SSCs.
Ahmadi M, Rahimi-Feyli P, Moghaddam AA, Alimohammadi S   +3 more
doaj  

Effect of storage temperature and osmotic pre-treatment with alternative solutes on the shelf-life of gilthead seabream (Sparus aurata) fillets

Aquaculture and Fisheries, 2017
The objective of the study was the kinetic modelling of the shelf-life of osmotically pre-treated fish during refrigerated and super-chilled storage. Fresh gilthead seabream (Sparus aurata) fillets were treated for 0–360 min at 15 °C in osmotic solutions
Theofania N. Tsironi, Petros S. Taoukis
doaj   +1 more source

Cryopreservation of date palm tissue cultures by vitrification and assessment of genetic stability using ISSR analysis

Notulae Scientia Biologicae
In this work, a successful cryopreservation method of date palm tissue cultures by vitrification was established. Callus and somatic embryos of three date palm cultivars were exposed to dimethylsulfoxide (DMSO) and Plant Vitrification Solution 2 (PVS2 ...
Shawky A. BEKHEET, Ahmed M.M. GABR, Maiada M. EL-DAWAYATI, Mona M. IBRAHIM, Mohamed R. RADY   +4 more
doaj   +1 more source

The effect of extracellular ice and cryoprotective agents on the water permeability parameters of human sperm plasma membrane during freezing [PDF]

, 2000
Ram V. Devireddy
openalex   +1 more source