Results 251 to 260 of about 138,952 (282)
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Culture of normal human leukocytes.
JAMA, 1967Rapidly growing and apparently permanent cultures were established from hematopoietic cells from two staff members with no known malignant disease. The cell lines were similar to those derived from leukocytes from the peripheral blood of patients with malignancies of either hematopoietic or nonhematopoietic origin and to the lymphoblastoid cell lines ...
G E, Moore, R E, Gerner, H A, Franklin
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Culture of normal adult human melanocytes*
British Journal of Dermatology, 2006Melanocytes isolated from normal adult human skin were cultured in vitro. Separation of the epidermis from the dermis by trypsin flotation proved better than collagenase treatment for providing viable cultures of melanocytes with a minimum of fibroblast contamination.
H I, Nielsen, P, Don
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Cultures of normal human mammary cells
Cell Differentiation, 1976This paper describes a method of obtaining epithelial cells from large quantities of normal human breast tissue and the response of these cells in culture to lactogenic hormones. Suspensions of single cells and clusters of cells resembling normal ductal and alveolar structures were obtained by mechanical disaggregation and subsequent (3h) incubation of
E V, Gaffney +4 more
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Normal human glomerular cells in culture
The Journal of Pathology, 1978Reproducible patterns of cell outgrowth have been observed from isolated normal adult, human glomeruli grown in tissue culture. Three morphologically distinct cell populations designated Types I, II, and III have been observed in culture from 15 normal human kidneys.
S R, Holdsworth +3 more
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Muscle & Nerve, 1981
AbstractTwelve‐day normal and dystrophic chick embryo breast muscle cells were cultured for up to 14 days, using normal embryo extract in the culture medium. Fluorodeoxyuridine was added on day 3 to suppress fibroblast overgrowth. Three of 6 experiments with normal cells were severely infected with avian leucosis/sarcoma (ALS) virus particles.
D G, McConnell +4 more
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AbstractTwelve‐day normal and dystrophic chick embryo breast muscle cells were cultured for up to 14 days, using normal embryo extract in the culture medium. Fluorodeoxyuridine was added on day 3 to suppress fibroblast overgrowth. Three of 6 experiments with normal cells were severely infected with avian leucosis/sarcoma (ALS) virus particles.
D G, McConnell +4 more
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Tissue culture demyelination by normal human serum
Annals of Neurology, 1984AbstractSerum from all of 20 normal individuals induced some degree of demyelination when applied to well‐myelinated mouse cerebellum cultures. An intact complement sequence through C5 is required. Demyelinating activity was heat labile at 56°C for 30 minutes but was not destroyed at 50°C for 30 minutes (which inhibits properdin factor B and alternate ...
D H, Silberberg +2 more
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Endoscopically Guided Sinus Cultures in Normal Subjects
American Journal of Rhinology, 1999The study of healthy sinus flora has been pursued with Caldwell-Luc, antral puncture, and anterior rhinoscopy. Increasingly, nasal endoscopy has been used to obtain cultures, yet the significance of findings using this technique remains uncertain. Our ability to interpret the growing number of these cultures will be greatly enhanced by determining the
D M, Nadel, D C, Lanza, D W, Kennedy
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Organ Culture of Normal and Psoriatic Skin
Archives of Dermatology, 1968Two hundred and sixty-four explants of adult human skin from 17 normal subjects, and 24 explants of psoriatic skin from 2 patients have been grown in organ culture for up to 15 days. Growth was assessed histologically and by autoradiography after pulse labeling with tritiated thymidine. Three growth patterns were defined. Migration of basal cells, both
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Growth of normal human mammary cells in culture
In Vitro, 1980Reduction mammoplasty tissue was used to obtain short-term cultures of human epithelial cell populations. Digestion of tissue with collagenase and hyaluronidase resulted in cell clusters (organoids) resembling ductal and alveolar structures; these could be separated by filtration from the stromal components.
M, Stampfer, R C, Hallowes, A J, Hackett
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Gossypol effects on cultured normal and malignant melanocytes
In Vitro Cellular & Developmental Biology, 1986Gossypol, a polyphenolic compound known to interfere with spermatogenesis, was found to have differential cytotoxic effects on normal and malignant melanocytes in culture. Ultrastructurally it caused marked swelling and vacuolization of mitochondria; there was an almost complete loss of cristae but the outer mitochondrial membrane was retained.
F, Hu, K, Mah, D J, Teramura
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