Results 41 to 50 of about 311,436 (256)

Mapping the lipidomic secretome of the early equine embryo

Frontiers in Veterinary Science
The lipidomic secretions of embryos provide a unique opportunity to examine the cellular processes of the early conceptus. In this study we profiled lipids released by the early equine conceptus, using high-resolution mass spectrometry to detect ...
Edwina F. Lawson, Russell Pickford, Robert John Aitken, Zamira Gibb, Christopher G. Grupen, Aleona Swegen   +5 more
doaj   +1 more source

Novel 3D co-culture model for epithelial-stromal cells interaction in prostate cancer. [PDF]

PLoS ONE, 2013
Paracrine function is a major mechanism of cell-cell communication within tissue microenvironment in normal development and disease. In vitro cell culture models simulating tissue or tumor microenvironment are necessary tools to delineate epithelial ...
Xiaolan Fang, Sivanandane Sittadjody, Kenneth Gyabaah, Emmanuel C Opara, Kethandapatti C Balaji   +4 more
doaj   +1 more source

Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria [PDF]

, 2016
Here, we provide the first evidence of yeast strains assisted Ag/AgCl-NPs production in vitro. The formed nanoparticles were characterized by spectroscopic and electron microscopy approaches. UV-vis supported the biosynthesis. TEM analysis evidenced that
Almeida-Paes, Rodrigo, de Souza, Wanderley, Eugenio, Mateus, Farina, Marcos, Frasés, Susana, Lemgruber, Leandro, Lima, Luís Maurício T.R., Müller, Nathalia, Sant'Anna, Celso   +8 more
core   +1 more source

Short-term culture for acute myeloid leukemia blast cells

مجلة مركز بحوث التقنيات الاحيائية, 2010
This study was carried out to separate acute myeloid leukemia (AML) blast cells and studies their proliferation in short-term culture. The separation procedure include three steps; Ficoll gradient separation, depletion of macrophages and depletion of ...
Majeed A. Sabbah, AssKhulood Al-Samarraea, Muhammad Rafeeq, Ali Muslim   +3 more
doaj   +1 more source

Optimized Culture Conditions for Improved Growth and Functional Differentiation of Mouse and Human Colon Organoids

Frontiers in Immunology, 2021
Background & AimsDiligent side-by-side comparisons of how different methodologies affect growth efficiency and quality of intestinal colonoids have not been performed leaving a gap in our current knowledge.
Sarah S. Wilson, Martha Mayo, Terry Melim, Heather Knight, Lori Patnaude, Xiaoming Wu, Lucy Phillips, Susan Westmoreland, Robert Dunstan, Edda Fiebiger, Sonia Terrillon   +10 more
doaj   +1 more source

Pancreatic cancer-derived S-100A8 N-terminal peptide: a diabetes cause? [PDF]

, 2006
BACKGROUND: Our aim was to identify the pancreatic cancer diabetogenic peptide. METHODS: Pancreatic tumor samples from patients with (n=15) or without (n=7) diabetes were compared with 6 non-neoplastic pancreas samples using SDS-PAGE.
BALDO G, BASSO D, FALDA A, FLAGIELLO A, FOGAR P, GIUNCO S, GRECO E, NAVAGLIA F, PEDRAZZOLI S, PLEBANI M., PUCCI, PIETRO, VALERIO A, ZAMBON CF   +12 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Dynamic alterations in gene expression after Wnt-mediated induction of avian neural crest [PDF]

, 2005
The Wnt signaling pathway is important in the formation of neural crest cells in many vertebrates, but the downstream targets of neural crest induction by Wnt are largely unknown. Here, we examined quantitative changes in gene expression regulated by Wnt-
Bronner, Marianne E., Taneyhill, Lisa A.
core   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Purkinje cell maturation participates in the control of oligodendrocyte differentiation: role of sonic hedgehog and vitronectin. [PDF]

PLoS ONE, 2012
Oligodendrocyte differentiation is temporally regulated during development by multiple factors. Here, we investigated whether the timing of oligodendrocyte differentiation might be controlled by neuronal differentiation in cerebellar organotypic cultures.
Lamia Bouslama-Oueghlani, Rosine Wehrlé, Mohamed Doulazmi, Xiao Ru Chen, Fanny Jaudon, Yolande Lemaigre-Dubreuil, Isabelle Rivals, Constantino Sotelo, Isabelle Dusart   +8 more
doaj   +1 more source