Results 71 to 80 of about 312,868 (309)

Transepithelial electrical resistance (TEER): a functional parameter to monitor the quality of oviduct epithelial cells cultured on filter supports [PDF]

, 2015
Cultivation of oviduct epithelial cells on porous filters fosters in vivo-like morphology and functionality. However, due to the optical properties of the filter materials and the cells’ columnar shape, cell quality is hard to assess via light microscopy.
Chen, Shuai, Einspanier, Ralf, Schoen, Jennifer   +2 more
core   +2 more sources

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Effect of Multiple-Cycle Collections of Conditioned Media from Different Cell Sources towards Fibroblasts in In Vitro Wound Healing Model

Pharmaceutics
Conditioned media refers to a collection of the used cell culture media. The goal of this study was to evaluate the possible impacts of different conditioned media collected across a number of cycles on the fibroblast proliferation, migration, and ...
Nur Izzah Md Fadilah, Mh Busra Fauzi, Manira Maarof   +2 more
doaj   +1 more source

Stem Cell Conditioned Culture Media Attenuated Albumin-Induced Epithelial-Mesenchymal Transition in Renal Tubular Cells

Cellular Physiology and Biochemistry, 2015
Background: Proteinuria-induced epithelial-mesenchymal transition (EMT) plays an important role in progressive renal tubulointerstitial fibrosis in chronic renal disease. Stem cell therapy has been used for different diseases.
Junping Hu, Qing Zhu, Pin-Lan Li, Weili Wang, Fan Yi, Ningjun Li   +5 more
doaj   +1 more source

Uterine selection of human embryos at implantation [PDF]

, 2014
Human embryos frequently harbor large-scale complex chromosomal errors that impede normal development. Affected embryos may fail to implant although many first breach the endometrial epithelium and embed in the decidualizing stroma before being rejected ...
A Mertzanidou, B Cloke, B Crespi, BC Han, BM Forman, CH Weimar, D Emera, D Emera, D Haig, DM Benbrook, E Rajcan-Separovic, E Vanneste, EB Chuong, EM Heijnen, FU Hartl, G Teklenburg, GW Wong, H Yang, HH Kampinga, JJ Brosens, JJ Brosens, LA Kazal, M Furuhashi, M Iwaki, M Ohmuraya, M Salker, MD Stephenson, MS Salker, MS Salker, N Arimura, N Mansouri-Attia, N Sharma, N Sharma, NS Macklon, P Walter, R Rai, S Altmae, S Kaushik, S Quenby, T Iwawaki, T Kadowaki, Y Kabeya, YC Ruan, YH Yang, YJ Kim   +44 more
core   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Effects of Feeder Layers, Culture Media, Conditional Media, Growth Factors, and Passages Number on Stem Cell Optimization [PDF]

, 2015
Stem cells are undifferentiated and self-renewal cells which could be obtained from the body or artificially derived from an adult somatic cell by forced expression of specific genes.
Abdian, Narges., Allahbakhshian-Farsani, Mehdi., Ghasemi-Dehkordi, Payam., Hashemzadeh-Chaleshtori, Morteza., Jafari-Ghahfarokhi, Hamideh., Mirzaeian, Amin.   +5 more
core  

Granulopoietic progenitors in suspension culture: a comparison of stimulatory cells and conditioned media [PDF]

Blood, 1975
Kinetic studies have been carried out to investigate the functional heterogeneity previously observed in populations of human marrow or peripheral blood cells separated by velocity sedimentation. The results obtained confirm the earlier results, in that slowly-sedimenting cells were found to stimulate both colony formation by granulopoietic progenitors
Niho, Y., Till, J. E., McCulloch, E. A.
openaire   +3 more sources

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Sensory and Motor Behavior Evidences Supporting the Usefulness of Conditioned Medium from Dental Pulp-Derived Stem Cells in Spinal Cord Injury in Rats [PDF]

Asian Spine Journal, 2018
Study Design Experimental animal study. Purpose This study aimed to assess effects of conditioned medium (CM) of dental pulp-derived stem cells loaded in collagen hydrogel on functional recovery following spinal cord injury (SCI).
Reza Asadi-Golshan, Vahid Razban, Esmaeil Mirzaei, Abdolkarim Rahmanian, Sahar Khajeh, Zohreh Mostafavi-Pour, Farzaneh Dehghani   +6 more
doaj   +1 more source