Development and validation of a targeted LC-MS/MS quantitation method to monitor cell culture expression of tetanus neurotoxin during vaccine production [PDF]
The tetanus neurotoxin (TeNT) is one of the most toxic proteins known to man, which prior to the use of the vaccine against the TeNT producing bacteria Clostridium tetani, resulted in a 20 % mortality rate upon infection. The clinical detrimental effects of tetanus have decreased immensely since the introduction of global vaccination programs, which ...
arxiv +1 more source
Collection time, dehidration, culture media and environment for germination and storage of campomanesia guazumifolia (cambess.) O. Berg. Pollen [PDF]
Sete-capote tree, a neglected species, has great ecological, cultural and potential economic importance, however, studies for this and other native species of the Myrtaceae family are still scarce.
Karina Guollo+4 more
doaj +1 more source
The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos [PDF]
Background: Somatic cell nuclear transfer (SCNT) is an approach for the propagation of elite animals. In vitro condition, especially the composition of culture media has a profound effect on the developmental competency of in vitro derived e mbryos ...
Mehdi Hajian+5 more
doaj +1 more source
Increasing the selectivity of cancer treatments is attractive, as it has the potential to reduce side-effects of therapy. Cold atmospheric plasma (CAP) is a novel cancer treatment that disrupts the intracellular oxidative balance.
Eline Biscop+8 more
semanticscholar +1 more source
Thyroid thermogenesis in adult rat hepatocytes in primary monolayer culture: direct action of thyroid hormone in vitro. [PDF]
We have studied the effect of 3,5,3'-triiodothyronine (T3) on the respiration of adult rat hepatocytes in primary monolayer culture prepared from hypothyroid rat liver. After addition of T3 to the culture medium at a concentration of 2 x 10(-7) M, oxygen
Bissell, DM+2 more
core +2 more sources
Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum
Exosomes are cell-derived vesicles and are abundant in biological fluids; they contain RNA molecules which may serve as potential diagnostic biomarkers in 'precision medicine'.
Yueting Tang+10 more
semanticscholar +1 more source
The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein ...
Adriana Cibele de Mesquita Dantas+3 more
doaj +1 more source
Extracellular vesicles (EVs) play a possible role in cell–cell communication and are found in various body fluids and cell conditioned culture media.
K. Pavani+8 more
semanticscholar +1 more source
A carnaúba hospedeira faz parte de um grupo de plantas da família Arecaceae, de relevante importância social e econômica para as regiões tropicais.
Alexandre Bosco de Oliveira+2 more
doaj +1 more source
Preservation of Indigenous Culture among Indigenous Migrants through Social Media: the Igorot Peoples [PDF]
The value and relevance of indigenous knowledge towards sustainability of human societies drives for its preservation. This work explored the use of Facebook groups to promote indigenous knowledge among Igorot peoples in the diaspora. The virtual communities help intensify the connection of Igorot migrants to their traditional culture despite the ...
arxiv +1 more source