Results 61 to 70 of about 807 (142)

Adaptive Laboratory Evolution of Cupriavidus necator to Improve Energy Demand in Bioelectrochemical Cultivations

ChemElectroChem, Volume 13, Issue 5, March 2026.
This study highlights biological improvement through adaptive laboratory evolution of Cupriavidus necator H16 ΔPHB. Adapted strains tolerated up to 400 mM Na2SO4 showing better growth under salt stress. This enhanced robustness reduced cultivation energy demand in bioelectrochemical systems by ~ 11%, enabling more sustainable bioelectrochemical ...
Lisa van der Sande, Manuel Müller, Dirk Holtmann   +2 more
wiley   +1 more source

Toward a synthetic hydrogen sensor in cyanobacteria: Functional production of an oxygen-tolerant regulatory hydrogenase in Synechocystis sp. PCC 6803

Frontiers in Microbiology, 2023
Cyanobacteria have raised great interest in biotechnology, e.g., for the sustainable production of molecular hydrogen (H2) using electrons from water oxidation. However, this is hampered by various constraints.
Franz Opel, Marvin Amadeus Itzenhäuser, Isabel Wehner, Sara Lupacchini, Lars Lauterbach, Oliver Lenz, Stephan Klähn   +6 more
doaj   +1 more source

Cost‐Efficient Autotrophic High‐Cell‐Density Cultivation of Cupriavidus necator Enabled by Model‐Based Gas Supply

Biotechnology and Bioengineering, Volume 123, Issue 1, Page 92-103, January 2026.
ABSTRACT Cultivating hydrogen‐oxidizing bacteria (HOB), such as Cupriavidus necator, using H 2, CO 2, and O 2 offers a promising route for CO 2 valorization into chemicals and materials. To enhance cultivation efficiency in a lab‐scale gas fermenter lacking a gas recycling system, an automated gas supply strategy based on real‐time CO 2 and O 2 ...
Vera Lambauer, Clemens Hagenbuchner, Maximilian Graber, Helmar Wiltsche, Vanja Subotić, Christoph Hochenauer, Markus Reichhartinger, Regina Kratzer   +7 more
wiley   +1 more source

Biological Conversion of Formate to Organic Compounds: Toward a Sustainable Formate Bioeconomy

Carbon Energy, Volume 8, Issue 1, January 2026.
Formate bioconversion plays a crucial role in achieving renewable resource utilization and sustainable development. To tap its full potential, it is important to identify the most appropriate microbial hosts for incorporating formate into building blocks, design the most promising metabolic pathways for transmitting formate into central carbon ...
Jinyi Qian, Tiantian Chai, Chunlei Zhao, Xiulai Chen   +3 more
wiley   +1 more source

Enzyme expression in Cupriavidus necator H16 for whole-cell biocatalysis

Climate change is an urgent and collective challenge, and new processes to synthesize complex molecules in a more sustainable way are highly desirable. Biocatalysis can be a strong player in this field, due to the specificity of enzymes and their ability to catalyze complex reactions at mild conditions.
Vajente, Matteo, Ghirardi, Mattia, Schmidt, Sandy   +2 more
openaire   +2 more sources

Rieske Oxygenase‐Catalyzed Biotransformations in Recombinant Cupriavidus necator Fueled by Formate Oxidation

ChemBioChem, Volume 26, Issue 21, November 8, 2025.
In this study, formatotrophic biotransformations in recombinant Cupriavidus necator catalyzed by Rieske oxygenases (ROs) toward various oxyfunctionalized olefins are demonstrated. The oxidation of formate regenerates NADH, the ROs’ cofactor, essentially fueling the reaction.
Marleen Hallamaa, Hannah Pia Franziska Meier, Matteo Vajente, Mattia Ghirardi, Jan Deska, Sandy Schmidt   +5 more
wiley   +1 more source

Establishing Mixotrophic Growth of Cupriavidus necator H16 on CO₂ and Volatile Fatty Acids

Fermentation, 2022
The facultative chemolithoautotroph Cupriavidus necator H16 is able to grow aerobically either with organic substrates or H2 and CO2 s and it can accumulate large amounts of (up to 90%) poly (3-hydroxybutyrate), a polyhydroxyalkanoate (PHA) biopolymer ...
Kamran Jawed, Victor Uhunoma Irorere, Rajesh Reddy Bommareddy, Nigel P. Minton, Katalin Kovács   +4 more
doaj   +1 more source

Response to Nutrient Stress in the Industrial Model Bacterium Cupriavidus necator: A Thermal Proteome Profiling (TPP) Investigation

PROTEOMICS, Volume 25, Issue 20, Page 25-38, October 2025.
ABSTRACT The facultative chemolithoautotroph Cupriavidus necator is capable of heterotrophic growth on diverse carbon sources, or of autotrophic growth using CO2 fixation with H2 as an energy source. Under stress conditions, it produces biodegradable polyesters (polyhydroxyalkanoates, PHAs) as a storage material occupying a high proportion of the total
Kate McKeever, Jia‐Lynn Tham, Manuel Bruch, Tania Narancic, Kevin O’ Connor, Swathi Ramachandra Upadhya, Colm Ryan, Eugene T. Dillon, Kieran Wynne, Gerard Cagney   +9 more
wiley   +1 more source

Hydrogen Oxidation Benefits Alphaproteobacterial Methanotrophs Under Severe Methane Limitation

Environmental Microbiology, Volume 27, Issue 8, August 2025.
We cultivated alphaproteobacterial methanotrophs under severe CH4‐limited conditions and studied their H2 oxidation characteristics as an alternative energy source. ABSTRACT Hydrogen (H2) and methane (CH4) are produced in the anoxic layers of wetlands and sediments. In the overlaying oxygenated surface layers, these gases become available for oxidation
Ida F. Peterse, Arjan Pol, Geert Cremers, Tom Berben, Theo A. van Alen, Huub J. M. Op den Camp, Annelies J. Veraart, Sebastian Lücker   +7 more
wiley   +1 more source

Microbial Electrosynthesis for Sustainable Polyhydroxybutyrate Production from CO2 Using Bismuth Nanoparticles

ChemElectroChem, Volume 12, Issue 14, July 10, 2025.
Electrochemical CO2 reduction using a Bi‐based electrode generates formate as the sole carbon source for Cupriavidus necator to synthesize polyhydroxybutyrate (PHB). Two configurations are evaluated: the integrated system, performing fermentation in the same reactor post‐electrolysis, while the drop‐in system decouples both steps.
Aliyah Aliyah, Filemon Jalu Nusantara Putra, Hiro Minamimoto, Yutaro Mori, Prihardi Kahar, Muhammad Iqbal Syauqi, Chiaki Ogino   +6 more
wiley   +1 more source