Results 41 to 50 of about 1,209 (160)
Fast‐track adaptive laboratory evolution of Cupriavidus necator H16 with divalent metal cations
Biotechnology Journal, Volume 19, Issue 7, July 2024.Graphical Abstract and Lay Summary Accelerated Adaptive Laboratory Evolution (aALE) is a novel tool designed to optimize microbial strains. This method enhances genetic diversity by utilizing divalent metal cations, which reduce the fidelity of genome replication, thereby speeding up the evolution process.
Sepwin Nosten Sitompul +4 more
wiley +4 more sources
The DUF81 protein TauE in Cupriavidus necator H16, a sulfite exporter in the metabolism of C2 sulfonates [PDF]
Microbiology, 2007 The degradation of taurine, isethionate and sulfoacetate in Cupriavidus necator (Ralstonia eutropha) H16 was shown by enzyme assays to be inducible, and each pathway involved sulfoacetaldehyde, which was subject to phosphatolysis by a common sulfoacetaldehyde acetyltransferase (Xsc, H16_B1870) to yield acetyl phosphate and sulfite.
Weinitschke, Sonja +3 more
openaire +4 more sources
MOESM8 of The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16
, 2019 Additional file 8: Figure S7. Growth of C. necator H16 and CNCA15 (ΔprpRBCMD) mutant strains on fructose and valine. Strains H16 (A) and CNCA15 (B) were grown on MM agar plates containing 25 mM fructose (a, left panel) and 30 mM valine (b, right panel). Agar plates were incubated at 30 °C for 5 days.
Arenas-López, Christian +8 more
openaire +2 more sources
Applying Statistical Design of Experiments To Understanding the Effect of Growth Medium Components on Cupriavidus necator H16 Growth [PDF]
Applied and Environmental Microbiology, 2020 Christopher C Azubuike +2 more
exaly +2 more sources
MOESM7 of The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16
, 2019 Additional file 7: Figure S6. Genetic complementation of CNCA16 (Δhbdh), CNCA05 (ΔmmsA3) and CNCA13 (ΔmmsA1ΔmmsA2ΔmmsA3) mutant strains restores growth on valine. (a) Growth of C. necator H16 wild type, CNCA16 (ΔhbdH) mutant and complemented CNCA16 (ΔhbdH) mutant.
Arenas-López, Christian +8 more
openaire +2 more sources
pCAT vectors overcome inefficient electroporation of Cupriavidus necator H16
New Biotechnology, 2021 Cupriavidus necator H16 is a chemolithoautotroph with a range of industrial biotechnological applications. Advanced metabolic engineering in the bacterium, however, is impeded by low transformation efficiency, making it difficult to introduce and screen new genetic functions rapidly. This study systematically characterized the broad host range plasmids
Christopher C. Azubuike +2 more
openaire +3 more sources
Frontiers in Bioengineering and Biotechnology, 2021 Cupriavidus necator strain A-04 has shown 16S rRNA gene identity to the well-known industrial strain C. necator H16. Nevertheless, the cell characteristics and polyhydroxyalkanoate (PHA) production ability of C.
Suchada Chanprateep Napathorn +6 more
doaj +1 more source
Molecular genetics and biochemistry of N-acetyltaurine degradation by Cupriavidus necator H16 [PDF]
Microbiology, 2011 Cupriavidus necator H16 (DSM 428), whose genome has been sequenced, was found to degrade N-acetyltaurine as a sole source of carbon and energy for growth. Utilization of the compound was quantitative. The degradative pathway involved an inducible N-acetyltaurine amidohydrolase (NaaS), which catalysed the cleavage of N-acetyltaurine ...
Karin, Denger +2 more
openaire +2 more sources
Scientific Reports, 2023 Bacterial pilin nanowires are protein complexes, suggested to possess electroactive capabilities forming part of the cells’ bioenergetic programming.
Ben Myers +5 more
doaj +1 more source
PLoS ONE, 2010 BackgroundCupriavidus necator JMP134 is a Gram-negative beta-proteobacterium able to grow on a variety of aromatic and chloroaromatic compounds as its sole carbon and energy source.Methodology/principal findingsIts genome consists of four replicons (two ...
Athanasios Lykidis +10 more
doaj +1 more source