Results 31 to 40 of about 807 (142)

Complete Genome Sequence of Cupriavidus necator H16 (DSM 428). [PDF]

open access: yesMicrobiol Resour Announc, 2019
The hydrogen-utilizing strain Cupriavidus necator H16 (DSM 428) was sequenced using a combination of PacBio and Illumina sequencing. Annotation of this strain reveals 6,543 protein-coding genes, 263 pseudogenes, 64 tRNA genes, and 15 rRNA genes.
Little GT   +6 more
europepmc   +3 more sources

The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16.

open access: yesJ Microbiol Methods, 2021
Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy
Ehsaan M   +4 more
europepmc   +4 more sources

Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2 [PDF]

open access: yesTrends in Biotechnology, 2021
Decelerating global warming is one of the predominant challenges of our time and will require conversion of CO2 to usable products and commodity chemicals. Of particular interest is the production of fuels, because the transportation sector is a major source of CO2 emissions. Here, we review recent technological advances in metabolic engineering of the
Justin Panich   +2 more
openaire   +4 more sources

pCAT vectors overcome inefficient electroporation of Cupriavidus necator H16

open access: yesNew Biotechnology, 2021
Cupriavidus necator H16 is a chemolithoautotroph with a range of industrial biotechnological applications. Advanced metabolic engineering in the bacterium, however, is impeded by low transformation efficiency, making it difficult to introduce and screen new genetic functions rapidly. This study systematically characterized the broad host range plasmids
Christopher C. Azubuike   +2 more
openaire   +3 more sources

Adaptive Laboratory Evolution of Cupriavidus necator H16 for Carbon Co-Utilization with Glycerol. [PDF]

open access: yesInt J Mol Sci, 2019
Cupriavidus necator H16 is a non-pathogenic Gram-negative betaproteobacterium that can utilize a broad range of renewable heterotrophic resources to produce chemicals ranging from polyhydroxybutyrate (biopolymer) to alcohols, alkanes, and alkenes. However, C.
González-Villanueva M   +6 more
europepmc   +6 more sources

Polyhydroxybutyrate (PHB) Production Using an Arabinose-Inducible Expression System in Comparison With Cold Shock Inducible Expression System in Escherichia coli

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Cupriavidus necator strain A-04 has shown 16S rRNA gene identity to the well-known industrial strain C. necator H16. Nevertheless, the cell characteristics and polyhydroxyalkanoate (PHA) production ability of C.
Suchada Chanprateep Napathorn   +6 more
doaj   +1 more source

Molecular genetics and biochemistry of N-acetyltaurine degradation by Cupriavidus necator H16 [PDF]

open access: yesMicrobiology, 2011
Cupriavidus necator H16 (DSM 428), whose genome has been sequenced, was found to degrade N-acetyltaurine as a sole source of carbon and energy for growth. Utilization of the compound was quantitative. The degradative pathway involved an inducible N-acetyltaurine amidohydrolase (NaaS), which catalysed the cleavage of N-acetyltaurine ...
Karin, Denger   +2 more
openaire   +2 more sources

Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production

open access: yesMetabolic Engineering, 2022
Cupriavidus necator H16 is one of the most researched carbon dioxide (CO2)-fixing bacteria. It can store carbon in form of the polymer polyhydroxybutyrate and generate energy by aerobic hydrogen oxidation under lithoautotrophic conditions, making C.
Hanko, Erik KR.   +3 more
openaire   +2 more sources

Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships

open access: yesScientific Reports, 2023
Bacterial pilin nanowires are protein complexes, suggested to possess electroactive capabilities forming part of the cells’ bioenergetic programming.
Ben Myers   +5 more
doaj   +1 more source

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