The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16 [PDF]
Biotechnology for Biofuels, 2019 Background 3-Hydroxypropionic acid (3-HP) is a promising platform chemical with various industrial applications. Several metabolic routes to produce 3-HP from organic substrates such as sugars or glycerol have been implemented in yeast, enterobacterial ...
Christian Arenas-López +8 more
doaj +5 more sources
Carbon dioxide valorization into resveratrol via lithoautotrophic fermentation using engineered Cupriavidus necator H16 [PDF]
Microbial Cell FactoriesBackground Industrial biomanufacturing of value-added products using CO2 as a carbon source is considered more sustainable, cost-effective and resource-efficient than using common carbohydrate feedstocks.
Yongjae Jang +6 more
doaj +3 more sources
Correction: H2-driven xylitol production in Cupriavidus necator H16 [PDF]
Microbial Cell FactoriesIn the methods section, the phrase “flushing with 100% H2 at 0.5 mL/min for 2 min.” should read “flushing with 100% H2 at 0.5 L/min for 2 min.”
Tytti Jämsä +3 more
doaj +4 more sources
The Overexpression of Phasin and Regulator Genes Promoting the Synthesis of Polyhydroxybutyrate in Cupriavidus necator H16 under Nonstress Conditions [PDF]
Applied and Environmental Microbiology, 2022 Cupriavidus necator H16 is an ideal strain for polyhydroxybutyrate (PHB) production from CO2. Low-oxygen stress can induce PHB synthesis in C. necator H16 while reducing bacterial growth under chemoautotrophic culture.
Xiaowei Peng, Yejun Han
exaly +3 more sources
Complete Genome Sequence of Cupriavidus necator H16 (DSM 428). [PDF]
Microbiol Resour Announc, 2019 The hydrogen-utilizing strain Cupriavidus necator H16 (DSM 428) was sequenced using a combination of PacBio and Illumina sequencing. Annotation of this strain reveals 6,543 protein-coding genes, 263 pseudogenes, 64 tRNA genes, and 15 rRNA genes.
Little GT +6 more
europepmc +5 more sources
Revisiting the single cell protein application of Cupriavidus necator H16 and recovering bioplastic granules simultaneously. [PDF]
PLoS ONE, 2013 Cupriavidus necator H16 (formerly known as Hydrogenomonas eutropha) was famous as a potential single cell protein (SCP) in the 1970s. The drawback however was the undesirably efficient accumulation of non-nutritive polyhydroxybutyrate (PHB) storage ...
Balakrishnan Kunasundari +4 more
doaj +3 more sources
Efficient biochemical production of acetoin from carbon dioxide using Cupriavidus necator H16 [PDF]
Biotechnology for Biofuels, 2019 Background Cupriavidus necator is the best-studied knallgas (also termed hydrogen oxidizing) bacterium and provides a model organism for studying the production of the storage polymer polyhydroxybutyrate (PHB).
Carina Windhorst, Johannes Gescher
doaj +6 more sources
The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16.
J Microbiol Methods, 2021 Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy
Ehsaan M +4 more
europepmc +7 more sources
Functional Genetic Elements for Controlling Gene Expression in Cupriavidus necator H16 [PDF]
Applied and Environmental Microbiology, 2018 This report provides tools for robust and predictable control of gene expression in the model lithoautotroph C. necator H16. To address a current need, we designed, built, and tested promoters and RBSs for controlling gene expression in C. necator H16. To answer a question on how existing and
Swathi Alagesan +2 more
exaly +7 more sources
Medium-Chain-Length Fatty Acid Catabolism in Cupriavidus necator H16: Transcriptome Sequencing Reveals Differences from Long-Chain-Length Fatty Acid β-Oxidation and Involvement of Several Homologous Genes [PDF]
Applied and Environmental Microbiology, 2023 Although Cupriavidus necator H16 has been used in several studies to produce polyhydroxyalkanoates from various lipids, the fatty acid metabolism is poorly understood.
Carl Simon Strittmatter +2 more
exaly +3 more sources