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l-amino acids affect the hydrogenase activity and growth of Ralstonia eutropha H16 [PDF]

open access: yesAMB Express, 2023
Keypoints 1 l-AAs supplementation enhanced the biomass of R. eutropha H16. 2 The presence of l-AA during bacterial growth caused a decrease in ORP and pH. 3 l-AA is suggested to promote Hyds synthesis and enzymatic activity by reducing ORP and pH.
Meri Iskandaryan   +5 more
doaj   +2 more sources

Optimization of Culture Conditions for Oxygen-Tolerant Regulatory [NiFe]-Hydrogenase Production from Ralstonia eutropha H16 in Escherichia coli [PDF]

open access: yesMicroorganisms, 2021
Hydrogenases are abundant metalloenzymes that catalyze the reversible conversion of molecular H2 into protons and electrons. Important achievements have been made over the past two decades in the understanding of these highly complex enzymes.
Qin Fan   +5 more
doaj   +2 more sources

Production of fatty acids in Ralstonia eutropha H16 by engineering β-oxidation and carbon storage [PDF]

open access: yesPeerJ, 2015
Ralstonia eutropha H16 is a facultatively autotrophic hydrogen-oxidizing bacterium capable of producing polyhydroxybutyrate (PHB)-based bioplastics. As PHB’s physical properties may be improved by incorporation of medium-chain-length fatty acids (MCFAs),
Janice S. Chen   +5 more
doaj   +3 more sources

A Comprehensive Safety Assessment of Ralstonia eutropha H16 for Food Applications: Integrating Genomic, Phenotypic, and Toxicological Analyzes [PDF]

open access: yesMicroorganisms
Ralstonia eutropha H16, a metabolically versatile bacterium, has gained prominence as a microbial platform for sustainable bioproduction. While its capabilities in synthesizing single-cell proteins and biodegradable materials are well documented ...
Xiaoyan You   +8 more
doaj   +2 more sources

Microaerobic insights into production of polyhydroxyalkanoates containing 3-hydroxyhexanoate via native reverse β-oxidation from glucose in Ralstonia eutropha H16 [PDF]

open access: yesMicrobial Cell Factories
Background Ralstonia eutropha H16, a facultative chemolitoautotroph, is an important workhorse for bioindustrial production of useful compounds such as polyhydroxyalkanoates (PHAs).
Kai-Hee Huong   +2 more
doaj   +2 more sources

Nutritional Value and Food Safety Assessment of Single-Cell Protein Derived from Ralstonia eutropha for Food Applications [PDF]

open access: yesFoods
The growing global protein demand and environmental concerns from conventional animal agriculture have driven the exploration of sustainable alternative protein sources.
Xiaoyan You   +6 more
doaj   +2 more sources

Comparative Genomic Assessment of the Cupriavidus necator Species for One-Carbon Based Biomanufacturing. [PDF]

open access: yesMicrob Biotechnol
Cupriavidus necator is a promising microbe for one‐carbon biomanufacturing. Newly sequenced strains and a comparative assessment of all available genomes define further this species and its traits of interest. ABSTRACT The transition from a petroleum‐based manufacturing to biomanufacturing is an important step towards a sustainable bio‐economy.
Jespersen MG   +4 more
europepmc   +2 more sources

Lignin valorization to bioplastics with an aromatic hub metabolite-based autoregulation system [PDF]

open access: yesNature Communications
Exploring microorganisms with downstream synthetic advantages in lignin valorization is an effective strategy to increase target product diversity and yield. This study ingeniously engineers the non-lignin-degrading bacterium Ralstonia eutropha H16 (also
Yiquan Zhao   +12 more
doaj   +2 more sources

Continuous Supply of Non-Combustible Gas Mixture for Safe Autotrophic Culture to Produce Polyhydroxyalkanoate by Hydrogen-Oxidizing Bacteria

open access: yesBioengineering, 2022
Polyhydroxyalkanoates (PHAs) are eco-friendly plastics that are thermoplastic and biodegradable in nature. The hydrogen-oxidizing bacterium Ralstonia eutropha can biosynthesize poly[(R)-3-hydroxybutyrate] [P(3HB)], the most common PHA, from carbon ...
Yuki Miyahara   +3 more
doaj   +1 more source

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